Multiple signals modulate the activity of the complex sensor kinase TodS.
Bottom Line: In the presence of toluene, menadione reduced TodS activity whereas toluene did not stimulate activity in the presence of menadione.The opposing effects of menadione on glucose and toluene metabolism may be partially responsible for the interwoven regulation of both catabolic pathways.This work provides mechanistic detail on how complex sensor kinases integrate different types of signal molecules.
Affiliation: Department of Environmental Protection, Consejo Superior de Investigaciones Científicas, Estación Experimental del Zaidín, C/ Prof. Albareda 1, Granada, 18008, Spain.Show MeSH
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Mentions: The first experiment involved the assessment of TodS activity at constant menadione but varying toluene concentrations. Data show that toluene did not stimulate TodS activity in the presence of menadione (Fig. 8A). Subsequently, TodS activity at constant toluene but varying menadione concentration was measured. As shown in Fig. 8B, the addition of menadione to toluene-containing TodS resulted in a dose-dependent reduction of TodS activity, and the magnitude of reduction was comparable with that observed in the absence of toluene. These data demonstrate that menadione-mediated reduction of TodS activity dominates over the toluene-stimulated increase.
Affiliation: Department of Environmental Protection, Consejo Superior de Investigaciones Científicas, Estación Experimental del Zaidín, C/ Prof. Albareda 1, Granada, 18008, Spain.