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Multiple signals modulate the activity of the complex sensor kinase TodS.

Silva-Jiménez H, Ortega Á, García-Fontana C, Ramos JL, Krell T - Microb Biotechnol (2014)

Bottom Line: In the presence of toluene, menadione reduced TodS activity whereas toluene did not stimulate activity in the presence of menadione.The opposing effects of menadione on glucose and toluene metabolism may be partially responsible for the interwoven regulation of both catabolic pathways.This work provides mechanistic detail on how complex sensor kinases integrate different types of signal molecules.

View Article: PubMed Central - PubMed

Affiliation: Department of Environmental Protection, Consejo Superior de Investigaciones Científicas, Estación Experimental del Zaidín, C/ Prof. Albareda 1, Granada, 18008, Spain.

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Assessment of the mutual influence of toluene- and menadione-mediated modulation of TodS activity.A. TodS was incubated with 5, 25, 50 and 100 μM toluene (corresponding to 0.5, 2.5, 5 and 10 × EC50) in the absence or presence of 340 μM menadione (corresponding to 2 × EC50).B. TodS was incubated with 85, 425, 850 and 1700 μM menadione (corresponding to 0.5, 2.5, 5 and 10 × EC50) in the absence or presence of 20 μM toluene (corresponding to 2 × EC50). Experiments were carried out in 2 mM DTT-containing analysis buffer. Samples were then submitted to autophosphorylation assays.
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fig08: Assessment of the mutual influence of toluene- and menadione-mediated modulation of TodS activity.A. TodS was incubated with 5, 25, 50 and 100 μM toluene (corresponding to 0.5, 2.5, 5 and 10 × EC50) in the absence or presence of 340 μM menadione (corresponding to 2 × EC50).B. TodS was incubated with 85, 425, 850 and 1700 μM menadione (corresponding to 0.5, 2.5, 5 and 10 × EC50) in the absence or presence of 20 μM toluene (corresponding to 2 × EC50). Experiments were carried out in 2 mM DTT-containing analysis buffer. Samples were then submitted to autophosphorylation assays.

Mentions: The first experiment involved the assessment of TodS activity at constant menadione but varying toluene concentrations. Data show that toluene did not stimulate TodS activity in the presence of menadione (Fig. 8A). Subsequently, TodS activity at constant toluene but varying menadione concentration was measured. As shown in Fig. 8B, the addition of menadione to toluene-containing TodS resulted in a dose-dependent reduction of TodS activity, and the magnitude of reduction was comparable with that observed in the absence of toluene. These data demonstrate that menadione-mediated reduction of TodS activity dominates over the toluene-stimulated increase.


Multiple signals modulate the activity of the complex sensor kinase TodS.

Silva-Jiménez H, Ortega Á, García-Fontana C, Ramos JL, Krell T - Microb Biotechnol (2014)

Assessment of the mutual influence of toluene- and menadione-mediated modulation of TodS activity.A. TodS was incubated with 5, 25, 50 and 100 μM toluene (corresponding to 0.5, 2.5, 5 and 10 × EC50) in the absence or presence of 340 μM menadione (corresponding to 2 × EC50).B. TodS was incubated with 85, 425, 850 and 1700 μM menadione (corresponding to 0.5, 2.5, 5 and 10 × EC50) in the absence or presence of 20 μM toluene (corresponding to 2 × EC50). Experiments were carried out in 2 mM DTT-containing analysis buffer. Samples were then submitted to autophosphorylation assays.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4321377&req=5

fig08: Assessment of the mutual influence of toluene- and menadione-mediated modulation of TodS activity.A. TodS was incubated with 5, 25, 50 and 100 μM toluene (corresponding to 0.5, 2.5, 5 and 10 × EC50) in the absence or presence of 340 μM menadione (corresponding to 2 × EC50).B. TodS was incubated with 85, 425, 850 and 1700 μM menadione (corresponding to 0.5, 2.5, 5 and 10 × EC50) in the absence or presence of 20 μM toluene (corresponding to 2 × EC50). Experiments were carried out in 2 mM DTT-containing analysis buffer. Samples were then submitted to autophosphorylation assays.
Mentions: The first experiment involved the assessment of TodS activity at constant menadione but varying toluene concentrations. Data show that toluene did not stimulate TodS activity in the presence of menadione (Fig. 8A). Subsequently, TodS activity at constant toluene but varying menadione concentration was measured. As shown in Fig. 8B, the addition of menadione to toluene-containing TodS resulted in a dose-dependent reduction of TodS activity, and the magnitude of reduction was comparable with that observed in the absence of toluene. These data demonstrate that menadione-mediated reduction of TodS activity dominates over the toluene-stimulated increase.

Bottom Line: In the presence of toluene, menadione reduced TodS activity whereas toluene did not stimulate activity in the presence of menadione.The opposing effects of menadione on glucose and toluene metabolism may be partially responsible for the interwoven regulation of both catabolic pathways.This work provides mechanistic detail on how complex sensor kinases integrate different types of signal molecules.

View Article: PubMed Central - PubMed

Affiliation: Department of Environmental Protection, Consejo Superior de Investigaciones Científicas, Estación Experimental del Zaidín, C/ Prof. Albareda 1, Granada, 18008, Spain.

Show MeSH
Related in: MedlinePlus