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Microbial response to single-cell protein production and brewery wastewater treatment.

Lee JZ, Logan A, Terry S, Spear JR - Microb Biotechnol (2014)

Bottom Line: A pyrosequencing survey of the brewery treatment plant showed that each unit process selected for a unique microbial community.Notably, flow equalization basins were dominated by Prevotella, methanogenesis effluent had the highest levels of diversity, and clarifier wet-well samples were sources of sequences for the candidate bacterial phyla of TM7 and BD1-5.These diazotrophs are potentially useful as the basis of a SCP product for commercial feed production.

View Article: PubMed Central - PubMed

Affiliation: Department of Civil and Environmental Engineering, Colorado School of Mines, Golden, CO, USA.

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Phylogenetic diversity of full-length Sanger sequences related to rhizospheric diazotrophs detected from the pilot bioreactor and final product for sequences from (A) Azoarcus, Azonexus and Azospira clades, (B) Magnetospirillum and Telmatospirillum clades, (C) and Azospirillum clades. Symbols denoting bootstrap support values are for maximum likelihood analyses. Representative sequences for OTUs identified from this study are in bold.
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fig05: Phylogenetic diversity of full-length Sanger sequences related to rhizospheric diazotrophs detected from the pilot bioreactor and final product for sequences from (A) Azoarcus, Azonexus and Azospira clades, (B) Magnetospirillum and Telmatospirillum clades, (C) and Azospirillum clades. Symbols denoting bootstrap support values are for maximum likelihood analyses. Representative sequences for OTUs identified from this study are in bold.

Mentions: Phylogenetic trees of full-length Sanger 16S sequences from rhizospheric diazotrophs in Alphaproteobacteria and Betaproteobacteria are shown in Fig. 5 (full phylogenetic tree in Supporting Information Fig. S5). In the Betaproteobacteria (Fig. 5A), near full-length sequences were related to known isolates of Azospira sp., Azovibrio restrictus and Azonexus caeni (but not genera Azoarcus). In the Alphaproteobacteria (Fig. 5B and C), near full-length clone sequences were not related to any of the known rhizospheric strains for Magnetospirillum and were related to one strain of Azospirillum [Azospirillum fermentarium CC-LY743 isolated from a fermentation tank (Lin et al., 2013)]. Closest basic local assignment search tool (BLAST) matches were commonly from strains isolated in large part from either wastewater and microbial fuel cell sources (Kaksonen et al., 2004; Quan et al., 2006; Borole et al., 2009; de Cárcer et al., 2011; Croese et al., 2011; Sun et al., 2011), as well as rhizosphere studies (Ashida et al., 2010; Knief et al., 2012) for which rhizospheric diazotrophs are primarily associated.


Microbial response to single-cell protein production and brewery wastewater treatment.

Lee JZ, Logan A, Terry S, Spear JR - Microb Biotechnol (2014)

Phylogenetic diversity of full-length Sanger sequences related to rhizospheric diazotrophs detected from the pilot bioreactor and final product for sequences from (A) Azoarcus, Azonexus and Azospira clades, (B) Magnetospirillum and Telmatospirillum clades, (C) and Azospirillum clades. Symbols denoting bootstrap support values are for maximum likelihood analyses. Representative sequences for OTUs identified from this study are in bold.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4321374&req=5

fig05: Phylogenetic diversity of full-length Sanger sequences related to rhizospheric diazotrophs detected from the pilot bioreactor and final product for sequences from (A) Azoarcus, Azonexus and Azospira clades, (B) Magnetospirillum and Telmatospirillum clades, (C) and Azospirillum clades. Symbols denoting bootstrap support values are for maximum likelihood analyses. Representative sequences for OTUs identified from this study are in bold.
Mentions: Phylogenetic trees of full-length Sanger 16S sequences from rhizospheric diazotrophs in Alphaproteobacteria and Betaproteobacteria are shown in Fig. 5 (full phylogenetic tree in Supporting Information Fig. S5). In the Betaproteobacteria (Fig. 5A), near full-length sequences were related to known isolates of Azospira sp., Azovibrio restrictus and Azonexus caeni (but not genera Azoarcus). In the Alphaproteobacteria (Fig. 5B and C), near full-length clone sequences were not related to any of the known rhizospheric strains for Magnetospirillum and were related to one strain of Azospirillum [Azospirillum fermentarium CC-LY743 isolated from a fermentation tank (Lin et al., 2013)]. Closest basic local assignment search tool (BLAST) matches were commonly from strains isolated in large part from either wastewater and microbial fuel cell sources (Kaksonen et al., 2004; Quan et al., 2006; Borole et al., 2009; de Cárcer et al., 2011; Croese et al., 2011; Sun et al., 2011), as well as rhizosphere studies (Ashida et al., 2010; Knief et al., 2012) for which rhizospheric diazotrophs are primarily associated.

Bottom Line: A pyrosequencing survey of the brewery treatment plant showed that each unit process selected for a unique microbial community.Notably, flow equalization basins were dominated by Prevotella, methanogenesis effluent had the highest levels of diversity, and clarifier wet-well samples were sources of sequences for the candidate bacterial phyla of TM7 and BD1-5.These diazotrophs are potentially useful as the basis of a SCP product for commercial feed production.

View Article: PubMed Central - PubMed

Affiliation: Department of Civil and Environmental Engineering, Colorado School of Mines, Golden, CO, USA.

Show MeSH