Microbial response to single-cell protein production and brewery wastewater treatment.
Bottom Line: A pyrosequencing survey of the brewery treatment plant showed that each unit process selected for a unique microbial community.Notably, flow equalization basins were dominated by Prevotella, methanogenesis effluent had the highest levels of diversity, and clarifier wet-well samples were sources of sequences for the candidate bacterial phyla of TM7 and BD1-5.These diazotrophs are potentially useful as the basis of a SCP product for commercial feed production.
Affiliation: Department of Civil and Environmental Engineering, Colorado School of Mines, Golden, CO, USA.Show MeSH
Mentions: Phylogenetic trees of full-length Sanger 16S sequences from rhizospheric diazotrophs in Alphaproteobacteria and Betaproteobacteria are shown in Fig. 5 (full phylogenetic tree in Supporting Information Fig. S5). In the Betaproteobacteria (Fig. 5A), near full-length sequences were related to known isolates of Azospira sp., Azovibrio restrictus and Azonexus caeni (but not genera Azoarcus). In the Alphaproteobacteria (Fig. 5B and C), near full-length clone sequences were not related to any of the known rhizospheric strains for Magnetospirillum and were related to one strain of Azospirillum [Azospirillum fermentarium CC-LY743 isolated from a fermentation tank (Lin et al., 2013)]. Closest basic local assignment search tool (BLAST) matches were commonly from strains isolated in large part from either wastewater and microbial fuel cell sources (Kaksonen et al., 2004; Quan et al., 2006; Borole et al., 2009; de Cárcer et al., 2011; Croese et al., 2011; Sun et al., 2011), as well as rhizosphere studies (Ashida et al., 2010; Knief et al., 2012) for which rhizospheric diazotrophs are primarily associated.
Affiliation: Department of Civil and Environmental Engineering, Colorado School of Mines, Golden, CO, USA.