Limits...
A small RNA mediated regulation of a stress-activated retrotransposon and the tissue specific transposition during the reproductive period in Arabidopsis.

Matsunaga W, Ohama N, Tanabe N, Masuta Y, Masuda S, Mitani N, Yamaguchi-Shinozaki K, Ma JF, Kato A, Ito H - Front Plant Sci (2015)

Bottom Line: We found that transcriptional activation of ONSEN was regulated by a small interfering RNA (siRNA)-related pathway, and the activation could also be induced by oxidative stress.The transposition was also detected in the progeny, which originated from tissue that had differentiated after exposure to the HS.The results indicated that in some undifferentiated cells, transpositional activity could be maintained quite long after exposure to the HS.

View Article: PubMed Central - PubMed

Affiliation: Faculty of Science, Hokkaido University, Sapporo Japan.

ABSTRACT
Transposable elements (TEs) are key elements that facilitate genome evolution of the host organism. A number of studies have assessed the functions of TEs, which change gene expression in the host genome. Activation of TEs is controlled by epigenetic modifications such as DNA methylation and histone modifications. Several recent studies have reported that TEs can also be activated by biotic or abiotic stress in some plants. We focused on a Ty1/copia retrotransposon, ONSEN, that is activated by heat stress (HS) in Arabidopsis. We found that transcriptional activation of ONSEN was regulated by a small interfering RNA (siRNA)-related pathway, and the activation could also be induced by oxidative stress. Mutants deficient in siRNA biogenesis that were exposed to HS at the initial stages of vegetative growth showed transgenerational transposition. The transposition was also detected in the progeny, which originated from tissue that had differentiated after exposure to the HS. The results indicated that in some undifferentiated cells, transpositional activity could be maintained quite long after exposure to the HS.

No MeSH data available.


Related in: MedlinePlus

Transgenerational transpositions in the WT (A) and nrpd1(B) plants. Data for plants grown under long-day (LD) and short-day (SD) conditions are shown in the left panels and right panels, respectively. 7, 10, 14, and 21 denote plants that were exposed to HS for 24 h at 7, 10, 14, and 21 days after germination, respectively. NS, non-stressed plants.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4321352&req=5

Figure 7: Transgenerational transpositions in the WT (A) and nrpd1(B) plants. Data for plants grown under long-day (LD) and short-day (SD) conditions are shown in the left panels and right panels, respectively. 7, 10, 14, and 21 denote plants that were exposed to HS for 24 h at 7, 10, 14, and 21 days after germination, respectively. NS, non-stressed plants.

Mentions: Seedlings that were growing under either long-day or short-day conditions were exposed to heat treatment 7, 10, 14, and 21 days after germination. The flowering time of the stressed nrpd1 was slightly affected by the treatment (Figure 6). No new copy of ONSEN was detected in the WT progeny, which were growing under conditions that were neither long day nor short day (Figure 7A). Transgenerational transposition was observed in the progeny of nrpd1 plants that were grown under either long-day or short-day conditions (Figure 7B). These results suggested that transposition frequency is not affected by flowering time and that the new insertions were transmitted into the reproductive tissue even when 3-week-old nrpd1 plants were exposed to HS.


A small RNA mediated regulation of a stress-activated retrotransposon and the tissue specific transposition during the reproductive period in Arabidopsis.

Matsunaga W, Ohama N, Tanabe N, Masuta Y, Masuda S, Mitani N, Yamaguchi-Shinozaki K, Ma JF, Kato A, Ito H - Front Plant Sci (2015)

Transgenerational transpositions in the WT (A) and nrpd1(B) plants. Data for plants grown under long-day (LD) and short-day (SD) conditions are shown in the left panels and right panels, respectively. 7, 10, 14, and 21 denote plants that were exposed to HS for 24 h at 7, 10, 14, and 21 days after germination, respectively. NS, non-stressed plants.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4321352&req=5

Figure 7: Transgenerational transpositions in the WT (A) and nrpd1(B) plants. Data for plants grown under long-day (LD) and short-day (SD) conditions are shown in the left panels and right panels, respectively. 7, 10, 14, and 21 denote plants that were exposed to HS for 24 h at 7, 10, 14, and 21 days after germination, respectively. NS, non-stressed plants.
Mentions: Seedlings that were growing under either long-day or short-day conditions were exposed to heat treatment 7, 10, 14, and 21 days after germination. The flowering time of the stressed nrpd1 was slightly affected by the treatment (Figure 6). No new copy of ONSEN was detected in the WT progeny, which were growing under conditions that were neither long day nor short day (Figure 7A). Transgenerational transposition was observed in the progeny of nrpd1 plants that were grown under either long-day or short-day conditions (Figure 7B). These results suggested that transposition frequency is not affected by flowering time and that the new insertions were transmitted into the reproductive tissue even when 3-week-old nrpd1 plants were exposed to HS.

Bottom Line: We found that transcriptional activation of ONSEN was regulated by a small interfering RNA (siRNA)-related pathway, and the activation could also be induced by oxidative stress.The transposition was also detected in the progeny, which originated from tissue that had differentiated after exposure to the HS.The results indicated that in some undifferentiated cells, transpositional activity could be maintained quite long after exposure to the HS.

View Article: PubMed Central - PubMed

Affiliation: Faculty of Science, Hokkaido University, Sapporo Japan.

ABSTRACT
Transposable elements (TEs) are key elements that facilitate genome evolution of the host organism. A number of studies have assessed the functions of TEs, which change gene expression in the host genome. Activation of TEs is controlled by epigenetic modifications such as DNA methylation and histone modifications. Several recent studies have reported that TEs can also be activated by biotic or abiotic stress in some plants. We focused on a Ty1/copia retrotransposon, ONSEN, that is activated by heat stress (HS) in Arabidopsis. We found that transcriptional activation of ONSEN was regulated by a small interfering RNA (siRNA)-related pathway, and the activation could also be induced by oxidative stress. Mutants deficient in siRNA biogenesis that were exposed to HS at the initial stages of vegetative growth showed transgenerational transposition. The transposition was also detected in the progeny, which originated from tissue that had differentiated after exposure to the HS. The results indicated that in some undifferentiated cells, transpositional activity could be maintained quite long after exposure to the HS.

No MeSH data available.


Related in: MedlinePlus