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PIRIN2 stabilizes cysteine protease XCP2 and increases susceptibility to the vascular pathogen Ralstonia solanacearum in Arabidopsis.

Zhang B, Tremousaygue D, Denancé N, van Esse HP, Hörger AC, Dabos P, Goffner D, Thomma BP, van der Hoorn RA, Tuominen H - Plant J. (2014)

Bottom Line: Here we describe a function of the Arabidopsis PIRIN2 (PRN2) that is related to susceptibility to the bacterial plant pathogen Ralstonia solanacearum.The stabilization of XCP2 by PRN2 was also confirmed in planta.Like prn2 mutants, an xcp2 single knockout mutant and xcp2 prn2 double knockout mutant displayed decreased susceptibility to R. solanacearum, suggesting that stabilization of XCP2 by PRN2 underlies susceptibility to R. solanacearum in Arabidopsis.

View Article: PubMed Central - PubMed

Affiliation: Umeå Plant Science Centre (UPSC), Department of Plant Physiology, Umeå University, 901 87, Umeå, Sweden.

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Related in: MedlinePlus

PRN2 interacts genetically with XCP2 during R. solanacearum infection. (a) Plant resistance to R. solanacearum GMI1000. Mean disease indices (see explanation in Figure1c legend) ± SD are shown for 30 plants per genotype 5, 6 and 7 days post-inoculation (DPI). (b) Growth of R. solanacearum bacteria in the plants. Log transformed mean numbers of colony-forming units per gram of fresh weight, cfu/gfw ± SD are shown for three biological replicates per genotype. The asterisks indicate significant differences (P < 0.001) between WT (Col-0) and the other genotypes according to Student's t-test. The pathogen inoculation assay was repeated three times, and the R. solanacearum bacterial growth experiments twice, yielding similar results. (c) Representative symptoms in each genotype at 7 DPI. Disease indices of these particular plants are indicated above each plant.
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fig06: PRN2 interacts genetically with XCP2 during R. solanacearum infection. (a) Plant resistance to R. solanacearum GMI1000. Mean disease indices (see explanation in Figure1c legend) ± SD are shown for 30 plants per genotype 5, 6 and 7 days post-inoculation (DPI). (b) Growth of R. solanacearum bacteria in the plants. Log transformed mean numbers of colony-forming units per gram of fresh weight, cfu/gfw ± SD are shown for three biological replicates per genotype. The asterisks indicate significant differences (P < 0.001) between WT (Col-0) and the other genotypes according to Student's t-test. The pathogen inoculation assay was repeated three times, and the R. solanacearum bacterial growth experiments twice, yielding similar results. (c) Representative symptoms in each genotype at 7 DPI. Disease indices of these particular plants are indicated above each plant.

Mentions: To further investigate this dependence, we inoculated an xcp2 knockout mutant (SALK_010938) (Avci et al., 2008), prn2-2 and the double mutant prn2-2 xcp2 with R. solanacearum, using Nd-1 (a resistant Arabidopsis accession) as a control. The prn2-2, xcp2, and prn2-2 xcp2 mutants were all more resistant to the pathogen than the WT plants and fewer disease symptoms appeared in their leaves (Figure6a,c). Growth rates of the bacteria were also slower in xcp2, prn2-2 and prn2-2 xcp2 leaf tissues (Figure6b). These results indicate that PRN2 and XCP2 both participate in a genetic pathway that increases the susceptibility to R. solanacearum, and thus support involvement of PRN2-mediated XCP2 accumulation in this pathway.


PIRIN2 stabilizes cysteine protease XCP2 and increases susceptibility to the vascular pathogen Ralstonia solanacearum in Arabidopsis.

Zhang B, Tremousaygue D, Denancé N, van Esse HP, Hörger AC, Dabos P, Goffner D, Thomma BP, van der Hoorn RA, Tuominen H - Plant J. (2014)

PRN2 interacts genetically with XCP2 during R. solanacearum infection. (a) Plant resistance to R. solanacearum GMI1000. Mean disease indices (see explanation in Figure1c legend) ± SD are shown for 30 plants per genotype 5, 6 and 7 days post-inoculation (DPI). (b) Growth of R. solanacearum bacteria in the plants. Log transformed mean numbers of colony-forming units per gram of fresh weight, cfu/gfw ± SD are shown for three biological replicates per genotype. The asterisks indicate significant differences (P < 0.001) between WT (Col-0) and the other genotypes according to Student's t-test. The pathogen inoculation assay was repeated three times, and the R. solanacearum bacterial growth experiments twice, yielding similar results. (c) Representative symptoms in each genotype at 7 DPI. Disease indices of these particular plants are indicated above each plant.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4321228&req=5

fig06: PRN2 interacts genetically with XCP2 during R. solanacearum infection. (a) Plant resistance to R. solanacearum GMI1000. Mean disease indices (see explanation in Figure1c legend) ± SD are shown for 30 plants per genotype 5, 6 and 7 days post-inoculation (DPI). (b) Growth of R. solanacearum bacteria in the plants. Log transformed mean numbers of colony-forming units per gram of fresh weight, cfu/gfw ± SD are shown for three biological replicates per genotype. The asterisks indicate significant differences (P < 0.001) between WT (Col-0) and the other genotypes according to Student's t-test. The pathogen inoculation assay was repeated three times, and the R. solanacearum bacterial growth experiments twice, yielding similar results. (c) Representative symptoms in each genotype at 7 DPI. Disease indices of these particular plants are indicated above each plant.
Mentions: To further investigate this dependence, we inoculated an xcp2 knockout mutant (SALK_010938) (Avci et al., 2008), prn2-2 and the double mutant prn2-2 xcp2 with R. solanacearum, using Nd-1 (a resistant Arabidopsis accession) as a control. The prn2-2, xcp2, and prn2-2 xcp2 mutants were all more resistant to the pathogen than the WT plants and fewer disease symptoms appeared in their leaves (Figure6a,c). Growth rates of the bacteria were also slower in xcp2, prn2-2 and prn2-2 xcp2 leaf tissues (Figure6b). These results indicate that PRN2 and XCP2 both participate in a genetic pathway that increases the susceptibility to R. solanacearum, and thus support involvement of PRN2-mediated XCP2 accumulation in this pathway.

Bottom Line: Here we describe a function of the Arabidopsis PIRIN2 (PRN2) that is related to susceptibility to the bacterial plant pathogen Ralstonia solanacearum.The stabilization of XCP2 by PRN2 was also confirmed in planta.Like prn2 mutants, an xcp2 single knockout mutant and xcp2 prn2 double knockout mutant displayed decreased susceptibility to R. solanacearum, suggesting that stabilization of XCP2 by PRN2 underlies susceptibility to R. solanacearum in Arabidopsis.

View Article: PubMed Central - PubMed

Affiliation: Umeå Plant Science Centre (UPSC), Department of Plant Physiology, Umeå University, 901 87, Umeå, Sweden.

Show MeSH
Related in: MedlinePlus