PIRIN2 stabilizes cysteine protease XCP2 and increases susceptibility to the vascular pathogen Ralstonia solanacearum in Arabidopsis.
Bottom Line: Here we describe a function of the Arabidopsis PIRIN2 (PRN2) that is related to susceptibility to the bacterial plant pathogen Ralstonia solanacearum.The stabilization of XCP2 by PRN2 was also confirmed in planta.Like prn2 mutants, an xcp2 single knockout mutant and xcp2 prn2 double knockout mutant displayed decreased susceptibility to R. solanacearum, suggesting that stabilization of XCP2 by PRN2 underlies susceptibility to R. solanacearum in Arabidopsis.
Affiliation: Umeå Plant Science Centre (UPSC), Department of Plant Physiology, Umeå University, 901 87, Umeå, Sweden.Show MeSH
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Mentions: To further investigate this dependence, we inoculated an xcp2 knockout mutant (SALK_010938) (Avci et al., 2008), prn2-2 and the double mutant prn2-2 xcp2 with R. solanacearum, using Nd-1 (a resistant Arabidopsis accession) as a control. The prn2-2, xcp2, and prn2-2 xcp2 mutants were all more resistant to the pathogen than the WT plants and fewer disease symptoms appeared in their leaves (Figure6a,c). Growth rates of the bacteria were also slower in xcp2, prn2-2 and prn2-2 xcp2 leaf tissues (Figure6b). These results indicate that PRN2 and XCP2 both participate in a genetic pathway that increases the susceptibility to R. solanacearum, and thus support involvement of PRN2-mediated XCP2 accumulation in this pathway.
Affiliation: Umeå Plant Science Centre (UPSC), Department of Plant Physiology, Umeå University, 901 87, Umeå, Sweden.