PIRIN2 stabilizes cysteine protease XCP2 and increases susceptibility to the vascular pathogen Ralstonia solanacearum in Arabidopsis.
Bottom Line: Here we describe a function of the Arabidopsis PIRIN2 (PRN2) that is related to susceptibility to the bacterial plant pathogen Ralstonia solanacearum.The stabilization of XCP2 by PRN2 was also confirmed in planta.Like prn2 mutants, an xcp2 single knockout mutant and xcp2 prn2 double knockout mutant displayed decreased susceptibility to R. solanacearum, suggesting that stabilization of XCP2 by PRN2 underlies susceptibility to R. solanacearum in Arabidopsis.
Affiliation: Umeå Plant Science Centre (UPSC), Department of Plant Physiology, Umeå University, 901 87, Umeå, Sweden.Show MeSH
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Mentions: A competitive DCG-04 labeling assay, in which DCG-04 probe was added to XCP2-overexpressing N. benthamiana leaf extracts simultaneously with rPRN2 provided indications of PRN2's action mechanism. In this assay, XCP2 activity was surprisingly suppressed in the presence of PRN2 at the start of the time course, but recovered to the same or somewhat higher activity level at the end of the time course experiment (Figure4a). This suggests that PRN2 inhibits XCP2 activity, but the inhibition is reversible and eventually results in stabilization of XCP2.
Affiliation: Umeå Plant Science Centre (UPSC), Department of Plant Physiology, Umeå University, 901 87, Umeå, Sweden.