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Measurement of serum estrogen and estrogen metabolites in pre- and postmenopausal women with osteoarthritis using high-performance liquid chromatography-electrospray ionization-tandem mass spectrometry.

Gao WL, Wu LS, Zi JH, Wu B, Li YZ, Song YC, Cai DZ - Braz. J. Med. Biol. Res. (2014)

Bottom Line: We developed a high-performance liquid chromatography-electrospray ionization-tandem mass spectrometry (HPLC-ESI-MS/MS) method to measure the levels of six EMs (i.e., estrone, E2, estriol, 2-hydroxyestrone, 2-hydroxyestradiol, and 16a-hydroxyestrone) in healthy pre- and postmenopausal women and women with OA.Compared to healthy women, serum-free E2 was lower in the luteal and postmenopausal phases in women with OA, and total serum E2 was lower in postmenopausal women with OA.Moreover, compared to healthy women, total serum 2-hydroxyestradiol was higher in postmenopausal women with OA and total serum 2-hydroxyestrone was lower in both the luteal and follicular phases in women with OA.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopedics, Linyi People's Hospital of Shandong Province, Linyi, China.

ABSTRACT
Although 17β-estradiol (E2) deficiency has been linked to the development of osteoarthritis (OA) in middle-aged women, there are few studies relating other estrogens and estrogen metabolites (EMs) to this condition. We developed a high-performance liquid chromatography-electrospray ionization-tandem mass spectrometry (HPLC-ESI-MS/MS) method to measure the levels of six EMs (i.e., estrone, E2, estriol, 2-hydroxyestrone, 2-hydroxyestradiol, and 16a-hydroxyestrone) in healthy pre- and postmenopausal women and women with OA. This method had a precision ranging from 1.1 to 3.1% and a detection limit ranging from 10 to 15 pg. Compared to healthy women, serum-free E2 was lower in the luteal and postmenopausal phases in women with OA, and total serum E2 was lower in postmenopausal women with OA. Moreover, compared to healthy women, total serum 2-hydroxyestradiol was higher in postmenopausal women with OA and total serum 2-hydroxyestrone was lower in both the luteal and follicular phases in women with OA. In conclusion, our HPLC-ESI-MS/MS method allowed the measurement of multiple biochemical targets in a single assay, and, given its increased cost-effectiveness, simplicity, and speed relative to previous methods, this method is suitable for clinical studies.

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Product mass spectrum of EMs and internal standards. E1:estrone; E2: estradiol; E3: estriol; 2-OHE1:2-hydroxyestrone; 2-OHE2: 2-hydroxyestradiol; 16a-OHE1:16a-hydroxyestrone; chlormycetin.
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f03: Product mass spectrum of EMs and internal standards. E1:estrone; E2: estradiol; E3: estriol; 2-OHE1:2-hydroxyestrone; 2-OHE2: 2-hydroxyestradiol; 16a-OHE1:16a-hydroxyestrone; chlormycetin.

Mentions: For the phenolic hydroxyl group of estrogens as proton donors, the signal is known tobe more sensitive in the negative ion mode than in the positive ion mode. In thisstudy, an exteriorized needle pump mode was used for each standard solution ofE1, E2, E3, 2-OHE1, 2-OHE2,16a-OHE1, or chlormycetin. In negative ion mode, mass spectra weretypical and specific for each reference substance (Figure 3).


Measurement of serum estrogen and estrogen metabolites in pre- and postmenopausal women with osteoarthritis using high-performance liquid chromatography-electrospray ionization-tandem mass spectrometry.

Gao WL, Wu LS, Zi JH, Wu B, Li YZ, Song YC, Cai DZ - Braz. J. Med. Biol. Res. (2014)

Product mass spectrum of EMs and internal standards. E1:estrone; E2: estradiol; E3: estriol; 2-OHE1:2-hydroxyestrone; 2-OHE2: 2-hydroxyestradiol; 16a-OHE1:16a-hydroxyestrone; chlormycetin.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4321220&req=5

f03: Product mass spectrum of EMs and internal standards. E1:estrone; E2: estradiol; E3: estriol; 2-OHE1:2-hydroxyestrone; 2-OHE2: 2-hydroxyestradiol; 16a-OHE1:16a-hydroxyestrone; chlormycetin.
Mentions: For the phenolic hydroxyl group of estrogens as proton donors, the signal is known tobe more sensitive in the negative ion mode than in the positive ion mode. In thisstudy, an exteriorized needle pump mode was used for each standard solution ofE1, E2, E3, 2-OHE1, 2-OHE2,16a-OHE1, or chlormycetin. In negative ion mode, mass spectra weretypical and specific for each reference substance (Figure 3).

Bottom Line: We developed a high-performance liquid chromatography-electrospray ionization-tandem mass spectrometry (HPLC-ESI-MS/MS) method to measure the levels of six EMs (i.e., estrone, E2, estriol, 2-hydroxyestrone, 2-hydroxyestradiol, and 16a-hydroxyestrone) in healthy pre- and postmenopausal women and women with OA.Compared to healthy women, serum-free E2 was lower in the luteal and postmenopausal phases in women with OA, and total serum E2 was lower in postmenopausal women with OA.Moreover, compared to healthy women, total serum 2-hydroxyestradiol was higher in postmenopausal women with OA and total serum 2-hydroxyestrone was lower in both the luteal and follicular phases in women with OA.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopedics, Linyi People's Hospital of Shandong Province, Linyi, China.

ABSTRACT
Although 17β-estradiol (E2) deficiency has been linked to the development of osteoarthritis (OA) in middle-aged women, there are few studies relating other estrogens and estrogen metabolites (EMs) to this condition. We developed a high-performance liquid chromatography-electrospray ionization-tandem mass spectrometry (HPLC-ESI-MS/MS) method to measure the levels of six EMs (i.e., estrone, E2, estriol, 2-hydroxyestrone, 2-hydroxyestradiol, and 16a-hydroxyestrone) in healthy pre- and postmenopausal women and women with OA. This method had a precision ranging from 1.1 to 3.1% and a detection limit ranging from 10 to 15 pg. Compared to healthy women, serum-free E2 was lower in the luteal and postmenopausal phases in women with OA, and total serum E2 was lower in postmenopausal women with OA. Moreover, compared to healthy women, total serum 2-hydroxyestradiol was higher in postmenopausal women with OA and total serum 2-hydroxyestrone was lower in both the luteal and follicular phases in women with OA. In conclusion, our HPLC-ESI-MS/MS method allowed the measurement of multiple biochemical targets in a single assay, and, given its increased cost-effectiveness, simplicity, and speed relative to previous methods, this method is suitable for clinical studies.

Show MeSH
Related in: MedlinePlus