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Epilepsy-induced electrocardiographic alterations following cardiac ischemia and reperfusion in rats.

Tavares JG, Vasques ER, Arida RM, Cavalheiro EA, Cabral FR, Torres LB, Menezes-Rodrigues FS, Jurkiewicz A, Caricati-Neto A, Godoy CM, Gomes da Silva S - Braz. J. Med. Biol. Res. (2015)

Bottom Line: It was found that rats with epilepsy presented a significant reduction in atrioventricular block incidence following the ischemia and reperfusion procedure.During the reperfusion period, a significant reduction in the QT interval (P<0.01) was verified in the epilepsy group in relation to the control group.Our results indicate that rats submitted to an epilepsy model induced by pilocarpine presented electrical conductivity alterations of cardiac tissue, mainly during an AMI episode.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Farmacologia, Universidade Federal de São Paulo, São Paulo, SP, Brasil.

ABSTRACT
The present study evaluated electrocardiographic alterations in rats with epilepsy submitted to an acute myocardial infarction (AMI) model induced by cardiac ischemia and reperfusion. Rats were randomly divided into two groups: control (n=12) and epilepsy (n=14). It was found that rats with epilepsy presented a significant reduction in atrioventricular block incidence following the ischemia and reperfusion procedure. In addition, significant alterations were observed in electrocardiogram intervals during the stabilization, ischemia, and reperfusion periods of rats with epilepsy compared to control rats. It was noted that rats with epilepsy presented a significant increase in the QRS interval during the stabilization period in relation to control rats (P<0.01). During the ischemia period, there was an increase in the QRS interval (P<0.05) and a reduction in the P wave and QT intervals (P<0.05 for both) in rats with epilepsy compared to control rats. During the reperfusion period, a significant reduction in the QT interval (P<0.01) was verified in the epilepsy group in relation to the control group. Our results indicate that rats submitted to an epilepsy model induced by pilocarpine presented electrical conductivity alterations of cardiac tissue, mainly during an AMI episode.

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Images of the experiment with ischemia and reperfusion and ECGmeasurements.
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f01: Images of the experiment with ischemia and reperfusion and ECGmeasurements.

Mentions: After 120 days, animals from the control and epilepsy groups were submitted to an AMImodel induced by cardiac ischemia and reperfusion similar to that previouslydescribed (25). All rats were anesthetizedwith urethane (1.2 g/kg, ip) and fixed in the supine position. Afterintubation with a catheter (Jelco 14G, USA), rats were mechanically ventilated withroom air with a stroke volume of approximately 10 ml/kg (∼82 breaths min) using aventilator (Insight EFF 312, Brazil). Under clean dissection, indwelling polyethylenecatheters were inserted into a common carotid artery. The body temperature wasmaintained at 37.5°C with a heated operating platform and appropriate heating lamps,and was monitored routinely via a rectal thermometer. The electrocardiogram (ECG) wasrecorded using a biopotential amplifier by means of needle electrodes placedsubcutaneously on the limbs. The chest was opened with a left thoracotomy and theheart was gently exteriorized using pressure on the abdomen. A ligature (4/0 braidedsilk suture attached to a 10-mm micropoint reverse-cutting needle, Ethicon K-890H,USA) was placed around the left anterior descending coronary artery, approximately 2mm from its origin. A polyethylene tube was threaded over the suture and the heartwas replaced in the chest cavity with the ligature ends exteriorized. After astabilization period of 15 min, the ligature was tied, characterizing the ischemicperiod, and, following a period of coronary occlusion (10 min), the reperfusion wasobtained by cutting the suture (Figure 1).Successful ligation of the coronary artery was validated by ECG alterations (increasein R wave and ST segment) and reduction in arterial pressure (26,27).


Epilepsy-induced electrocardiographic alterations following cardiac ischemia and reperfusion in rats.

Tavares JG, Vasques ER, Arida RM, Cavalheiro EA, Cabral FR, Torres LB, Menezes-Rodrigues FS, Jurkiewicz A, Caricati-Neto A, Godoy CM, Gomes da Silva S - Braz. J. Med. Biol. Res. (2015)

Images of the experiment with ischemia and reperfusion and ECGmeasurements.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4321219&req=5

f01: Images of the experiment with ischemia and reperfusion and ECGmeasurements.
Mentions: After 120 days, animals from the control and epilepsy groups were submitted to an AMImodel induced by cardiac ischemia and reperfusion similar to that previouslydescribed (25). All rats were anesthetizedwith urethane (1.2 g/kg, ip) and fixed in the supine position. Afterintubation with a catheter (Jelco 14G, USA), rats were mechanically ventilated withroom air with a stroke volume of approximately 10 ml/kg (∼82 breaths min) using aventilator (Insight EFF 312, Brazil). Under clean dissection, indwelling polyethylenecatheters were inserted into a common carotid artery. The body temperature wasmaintained at 37.5°C with a heated operating platform and appropriate heating lamps,and was monitored routinely via a rectal thermometer. The electrocardiogram (ECG) wasrecorded using a biopotential amplifier by means of needle electrodes placedsubcutaneously on the limbs. The chest was opened with a left thoracotomy and theheart was gently exteriorized using pressure on the abdomen. A ligature (4/0 braidedsilk suture attached to a 10-mm micropoint reverse-cutting needle, Ethicon K-890H,USA) was placed around the left anterior descending coronary artery, approximately 2mm from its origin. A polyethylene tube was threaded over the suture and the heartwas replaced in the chest cavity with the ligature ends exteriorized. After astabilization period of 15 min, the ligature was tied, characterizing the ischemicperiod, and, following a period of coronary occlusion (10 min), the reperfusion wasobtained by cutting the suture (Figure 1).Successful ligation of the coronary artery was validated by ECG alterations (increasein R wave and ST segment) and reduction in arterial pressure (26,27).

Bottom Line: It was found that rats with epilepsy presented a significant reduction in atrioventricular block incidence following the ischemia and reperfusion procedure.During the reperfusion period, a significant reduction in the QT interval (P<0.01) was verified in the epilepsy group in relation to the control group.Our results indicate that rats submitted to an epilepsy model induced by pilocarpine presented electrical conductivity alterations of cardiac tissue, mainly during an AMI episode.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Farmacologia, Universidade Federal de São Paulo, São Paulo, SP, Brasil.

ABSTRACT
The present study evaluated electrocardiographic alterations in rats with epilepsy submitted to an acute myocardial infarction (AMI) model induced by cardiac ischemia and reperfusion. Rats were randomly divided into two groups: control (n=12) and epilepsy (n=14). It was found that rats with epilepsy presented a significant reduction in atrioventricular block incidence following the ischemia and reperfusion procedure. In addition, significant alterations were observed in electrocardiogram intervals during the stabilization, ischemia, and reperfusion periods of rats with epilepsy compared to control rats. It was noted that rats with epilepsy presented a significant increase in the QRS interval during the stabilization period in relation to control rats (P<0.01). During the ischemia period, there was an increase in the QRS interval (P<0.05) and a reduction in the P wave and QT intervals (P<0.05 for both) in rats with epilepsy compared to control rats. During the reperfusion period, a significant reduction in the QT interval (P<0.01) was verified in the epilepsy group in relation to the control group. Our results indicate that rats submitted to an epilepsy model induced by pilocarpine presented electrical conductivity alterations of cardiac tissue, mainly during an AMI episode.

Show MeSH
Related in: MedlinePlus