Limits...
Berberine induces apoptosis via ROS generation in PANC-1 and MIA-PaCa2 pancreatic cell lines.

Park SH, Sung JH, Kim EJ, Chung N - Braz. J. Med. Biol. Res. (2014)

Bottom Line: Whereas gemcitabine exerts antiproliferation effects through S-phase arrest, our results showed that berberine inhibited proliferation by inducing G1-phase arrest.Berberine-induced apoptosis of PANC-1 and MIA-PaCa2 cells increased by 7 and 2% compared to control cells, respectively.Notably, berberine had a greater apoptotic effect in PANC-1 cells than gemcitabine.

View Article: PubMed Central - PubMed

Affiliation: Division of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul, Korea.

ABSTRACT
Pancreatic cancer is the fourth leading cause of cancer death. Gemcitabine is widely used as a chemotherapeutic agent for the treatment of pancreatic cancer, but the prognosis is still poor. Berberine, an isoquinoline alkaloid extracted from a variety of natural herbs, possesses a variety of pharmacological properties including anticancer effects. In this study, we investigated the anticancer effects of berberine and compared its use with that of gemcitabine in the pancreatic cancer cell lines PANC-1 and MIA-PaCa2. Berberine inhibited cell growth in a dose-dependent manner by inducing cell cycle arrest and apoptosis. After berberine treatment, the G1 phase of PANC-1 cells increased by 10% compared to control cells, and the G1 phase of MIA-PaCa2 cells was increased by 2%. Whereas gemcitabine exerts antiproliferation effects through S-phase arrest, our results showed that berberine inhibited proliferation by inducing G1-phase arrest. Berberine-induced apoptosis of PANC-1 and MIA-PaCa2 cells increased by 7 and 2% compared to control cells, respectively. Notably, berberine had a greater apoptotic effect in PANC-1 cells than gemcitabine. Upon treatment of PANC-1 and MIA-PaCa2 with berberine at a half-maximal inhibitory concentration (IC50), apoptosis was induced by a mechanism that involved the production of reactive oxygen species (ROS) rather than caspase 3/7 activation. Our findings showed that berberine had anti-cancer effects and may be an effective drug for pancreatic cancer chemotherapy.

Show MeSH

Related in: MedlinePlus

Cell cycle after treatment of MIA-PaCa2 with berberine or gemcitabine.MIA-PaCa2 cells were treated with distilled water (control), 10 µM berberine,or 7 nM gemcitabine for 72 h. The cell cycle distribution was analyzed by theModFit LT software and depicted using histograms (A) and barplots (B). Cell cycle was analyzed as the percentage of cellsat each stage of the cell cycle after DNA staining with PI. Data from arepresentative experiment (from a total of at least three) are shown.*P<0.05, **P<0.01 versus control(Student's t-test).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4321216&req=5

f03: Cell cycle after treatment of MIA-PaCa2 with berberine or gemcitabine.MIA-PaCa2 cells were treated with distilled water (control), 10 µM berberine,or 7 nM gemcitabine for 72 h. The cell cycle distribution was analyzed by theModFit LT software and depicted using histograms (A) and barplots (B). Cell cycle was analyzed as the percentage of cellsat each stage of the cell cycle after DNA staining with PI. Data from arepresentative experiment (from a total of at least three) are shown.*P<0.05, **P<0.01 versus control(Student's t-test).

Mentions: Cell cycle progression was examined after treatment with berberine. As shown in Figure 2, treatment of PANC-1 cells with 15 µMberberine for 72 h resulted in a significantly higher percentage (61.1±1.7%) of cellsin the G1 phase than in the control group (51.1±0.8%), with a corresponding reductionin the percentage of cells in the S phase. Similar results were obtained forMIA-PaCa2 (55.4±1.1%) when treated with 10 µM berberine for 72 h compared to thecontrol group (53.7±0.5%) (Figure 3). Thesedata suggest that inhibition of cell proliferation or induction of cell death inpancreatic cancer cells by berberine is associated with the induction of G1arrest.


Berberine induces apoptosis via ROS generation in PANC-1 and MIA-PaCa2 pancreatic cell lines.

Park SH, Sung JH, Kim EJ, Chung N - Braz. J. Med. Biol. Res. (2014)

Cell cycle after treatment of MIA-PaCa2 with berberine or gemcitabine.MIA-PaCa2 cells were treated with distilled water (control), 10 µM berberine,or 7 nM gemcitabine for 72 h. The cell cycle distribution was analyzed by theModFit LT software and depicted using histograms (A) and barplots (B). Cell cycle was analyzed as the percentage of cellsat each stage of the cell cycle after DNA staining with PI. Data from arepresentative experiment (from a total of at least three) are shown.*P<0.05, **P<0.01 versus control(Student's t-test).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4321216&req=5

f03: Cell cycle after treatment of MIA-PaCa2 with berberine or gemcitabine.MIA-PaCa2 cells were treated with distilled water (control), 10 µM berberine,or 7 nM gemcitabine for 72 h. The cell cycle distribution was analyzed by theModFit LT software and depicted using histograms (A) and barplots (B). Cell cycle was analyzed as the percentage of cellsat each stage of the cell cycle after DNA staining with PI. Data from arepresentative experiment (from a total of at least three) are shown.*P<0.05, **P<0.01 versus control(Student's t-test).
Mentions: Cell cycle progression was examined after treatment with berberine. As shown in Figure 2, treatment of PANC-1 cells with 15 µMberberine for 72 h resulted in a significantly higher percentage (61.1±1.7%) of cellsin the G1 phase than in the control group (51.1±0.8%), with a corresponding reductionin the percentage of cells in the S phase. Similar results were obtained forMIA-PaCa2 (55.4±1.1%) when treated with 10 µM berberine for 72 h compared to thecontrol group (53.7±0.5%) (Figure 3). Thesedata suggest that inhibition of cell proliferation or induction of cell death inpancreatic cancer cells by berberine is associated with the induction of G1arrest.

Bottom Line: Whereas gemcitabine exerts antiproliferation effects through S-phase arrest, our results showed that berberine inhibited proliferation by inducing G1-phase arrest.Berberine-induced apoptosis of PANC-1 and MIA-PaCa2 cells increased by 7 and 2% compared to control cells, respectively.Notably, berberine had a greater apoptotic effect in PANC-1 cells than gemcitabine.

View Article: PubMed Central - PubMed

Affiliation: Division of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul, Korea.

ABSTRACT
Pancreatic cancer is the fourth leading cause of cancer death. Gemcitabine is widely used as a chemotherapeutic agent for the treatment of pancreatic cancer, but the prognosis is still poor. Berberine, an isoquinoline alkaloid extracted from a variety of natural herbs, possesses a variety of pharmacological properties including anticancer effects. In this study, we investigated the anticancer effects of berberine and compared its use with that of gemcitabine in the pancreatic cancer cell lines PANC-1 and MIA-PaCa2. Berberine inhibited cell growth in a dose-dependent manner by inducing cell cycle arrest and apoptosis. After berberine treatment, the G1 phase of PANC-1 cells increased by 10% compared to control cells, and the G1 phase of MIA-PaCa2 cells was increased by 2%. Whereas gemcitabine exerts antiproliferation effects through S-phase arrest, our results showed that berberine inhibited proliferation by inducing G1-phase arrest. Berberine-induced apoptosis of PANC-1 and MIA-PaCa2 cells increased by 7 and 2% compared to control cells, respectively. Notably, berberine had a greater apoptotic effect in PANC-1 cells than gemcitabine. Upon treatment of PANC-1 and MIA-PaCa2 with berberine at a half-maximal inhibitory concentration (IC50), apoptosis was induced by a mechanism that involved the production of reactive oxygen species (ROS) rather than caspase 3/7 activation. Our findings showed that berberine had anti-cancer effects and may be an effective drug for pancreatic cancer chemotherapy.

Show MeSH
Related in: MedlinePlus