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Genetic association between methylenetetrahydrofolate reductase gene polymorphism and risk of osteonecrosis of the femoral head.

Chai W, Zhang Z, Ni M, Geng P, Lian Z, Zhang G, Shi LL, Chen J - Biomed Res Int (2015)

Bottom Line: Both fixed effects model and random effects model were used.We eventually included twelve studies in this analysis, with results showing no overall association between ONFH susceptibility and SNP rs1801133 (T versus C: OR=1.15, 95% CI=0.97-1.38; TT versus CC: OR=1.15, 95% CI=0.91-1.46; TT/CT versus CC: OR=1.09, 95% CI=0.95-1.25; and TT versus OR=1.16, 95% CI=0.93-1.45).When stratified based on ethnicity, the results were still not significant.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopaedics, Chinese People's Liberation Army General Hospital, 301 Orthopaedic Hospital, 28 Fuxing Road, Haidian District, Beijing 100853, China.

ABSTRACT

Background: Methylenetetrahydrofolate reductase (MTHFR) SNP rs1801133 has been frequently investigated in recent years. Relevant candidate gene association studies with this SNP and osteonecrosis of the femoral head (ONFH) reported conflicting results. Meta-analysis provides a method to combine these data and to determine the association in a larger sample size.

Method: We conducted a systematic search to identify possible studies. Four pooled ORs (odds ratios, T versus C, TT versus CC, TT/CT versus CC, and TT versus CT/CC), along with 95% confidence interval (CI), were calculated to evaluate the association between SNP rs1801133 and ONFH susceptibility. Both fixed effects model and random effects model were used.

Findings: We eventually included twelve studies in this analysis, with results showing no overall association between ONFH susceptibility and SNP rs1801133 (T versus C: OR=1.15, 95% CI=0.97-1.38; TT versus CC: OR=1.15, 95% CI=0.91-1.46; TT/CT versus CC: OR=1.09, 95% CI=0.95-1.25; and TT versus

Ct/cc: OR=1.16, 95% CI=0.93-1.45). When stratified based on ethnicity, the results were still not significant.

Conclusion: Our findings are generally supportive of no association between MTHFR SNP rs1801133 and the etiology of ONFH.

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Related in: MedlinePlus

Flow chart of study identification and specific reasons for exclusion from the meta-analysis.
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fig1: Flow chart of study identification and specific reasons for exclusion from the meta-analysis.

Mentions: Figure 1 summarizes the process of study identification. We yielded a total of 331 relevant articles (PubMed, 207; CNKI, 124). Evaluation of title, abstract, and full-text led to 12 eligible studies [14–18, 23–29]. General characteristics of the included studies are summarized in Table 1. Seven studies investigated the association of SNP rs1801133 with ONFH susceptibility in a sample of Caucasian population and five in Asian populations. Seven studies used healthy control subjects and four used ONFH-free subjects, with one not reporting such information. Most studies used polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) to genotype SNP rs1801133. Deviation from HWE was tested in three studies [15, 16, 26].


Genetic association between methylenetetrahydrofolate reductase gene polymorphism and risk of osteonecrosis of the femoral head.

Chai W, Zhang Z, Ni M, Geng P, Lian Z, Zhang G, Shi LL, Chen J - Biomed Res Int (2015)

Flow chart of study identification and specific reasons for exclusion from the meta-analysis.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4321101&req=5

fig1: Flow chart of study identification and specific reasons for exclusion from the meta-analysis.
Mentions: Figure 1 summarizes the process of study identification. We yielded a total of 331 relevant articles (PubMed, 207; CNKI, 124). Evaluation of title, abstract, and full-text led to 12 eligible studies [14–18, 23–29]. General characteristics of the included studies are summarized in Table 1. Seven studies investigated the association of SNP rs1801133 with ONFH susceptibility in a sample of Caucasian population and five in Asian populations. Seven studies used healthy control subjects and four used ONFH-free subjects, with one not reporting such information. Most studies used polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) to genotype SNP rs1801133. Deviation from HWE was tested in three studies [15, 16, 26].

Bottom Line: Both fixed effects model and random effects model were used.We eventually included twelve studies in this analysis, with results showing no overall association between ONFH susceptibility and SNP rs1801133 (T versus C: OR=1.15, 95% CI=0.97-1.38; TT versus CC: OR=1.15, 95% CI=0.91-1.46; TT/CT versus CC: OR=1.09, 95% CI=0.95-1.25; and TT versus OR=1.16, 95% CI=0.93-1.45).When stratified based on ethnicity, the results were still not significant.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopaedics, Chinese People's Liberation Army General Hospital, 301 Orthopaedic Hospital, 28 Fuxing Road, Haidian District, Beijing 100853, China.

ABSTRACT

Background: Methylenetetrahydrofolate reductase (MTHFR) SNP rs1801133 has been frequently investigated in recent years. Relevant candidate gene association studies with this SNP and osteonecrosis of the femoral head (ONFH) reported conflicting results. Meta-analysis provides a method to combine these data and to determine the association in a larger sample size.

Method: We conducted a systematic search to identify possible studies. Four pooled ORs (odds ratios, T versus C, TT versus CC, TT/CT versus CC, and TT versus CT/CC), along with 95% confidence interval (CI), were calculated to evaluate the association between SNP rs1801133 and ONFH susceptibility. Both fixed effects model and random effects model were used.

Findings: We eventually included twelve studies in this analysis, with results showing no overall association between ONFH susceptibility and SNP rs1801133 (T versus C: OR=1.15, 95% CI=0.97-1.38; TT versus CC: OR=1.15, 95% CI=0.91-1.46; TT/CT versus CC: OR=1.09, 95% CI=0.95-1.25; and TT versus

Ct/cc: OR=1.16, 95% CI=0.93-1.45). When stratified based on ethnicity, the results were still not significant.

Conclusion: Our findings are generally supportive of no association between MTHFR SNP rs1801133 and the etiology of ONFH.

Show MeSH
Related in: MedlinePlus