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Dragging human mesenchymal stem cells with the aid of supramolecular assemblies of single-walled carbon nanotubes, molecular magnets, and peptides in a magnetic field.

de Paula AC, Sáfar GA, Góes AM, Bemquerer MP, Ribeiro MA, Stumpf HO - Biomed Res Int (2015)

Bottom Line: There is a growing number of cell therapies using stem cells due to their characteristics of modulation of immune system and reduction of acute rejection.These assemblies were narrower than their related allotropic forms, that is, multiwalled carbon nanotubes, and they could therefore be used to guide cells through thin blood capillaries within the human body.This strategy seems to be useful as noninvasive and nontoxic stem cells delivery/guidance and tracking during cell therapy.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Bioquímica e Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, 31270-901 Belo Horizonte, MG, Brazil.

ABSTRACT
Human adipose-derived stem cells (hASCs) are an attractive cell source for therapeutic applicability in diverse fields for the repair and regeneration of damaged or malfunctioning tissues and organs. There is a growing number of cell therapies using stem cells due to their characteristics of modulation of immune system and reduction of acute rejection. So a challenge in stem cells therapy is the delivery of cells to the organ of interest, a specific site. The aim of this paper was to investigate the effects of a supramolecular assembly composed of single-walled carbon nanotubes (SWCNT), molecular magnets (lawsone-Co-phenanthroline), and a synthetic peptide (FWYANHYWFHNAFWYANHYWFHNA) in the hASCs cultures. The hASCs were isolated, characterized, expanded, and cultured with the SWCNT supramolecular assembly (SWCNT-MA). The assembly developed did not impair the cell characteristics, viability, or proliferation. During growth, the cells were strongly attached to the assembly and they could be dragged by an applied magnetic field of less than 0.3 T. These assemblies were narrower than their related allotropic forms, that is, multiwalled carbon nanotubes, and they could therefore be used to guide cells through thin blood capillaries within the human body. This strategy seems to be useful as noninvasive and nontoxic stem cells delivery/guidance and tracking during cell therapy.

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SWCNT-MA suspension orientation. (a) A suspension of SWCNT-MA in a glass vial. (b) The same suspension with an applied magnet field (originated by the NdFeB magnet at right).
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fig4: SWCNT-MA suspension orientation. (a) A suspension of SWCNT-MA in a glass vial. (b) The same suspension with an applied magnet field (originated by the NdFeB magnet at right).

Mentions: The SWCNT-MA suspension with and without an applied magnetic field is shown in Figure 4. The aggregation on the vial sidewall where the NdFeB magnet was left shows the ferromagnetism of the SWCNT-MA. To show the practical application of the method, we can make a comparison. For instance, a magnetic field of 3 T is currently used for patients undergoing MRI scans, without disturbing physiological functions [21, 22]. In our experiments, the field never surpassed 0.3 T, which is 10 times lower than that commonly used for MRI. In fact, the magnet used in the study has a 10-fold weaker field than a 3-Tesla magnet used in common MRI equipment. Hence, around a 3-fold larger distance can be expected to have a similar effect on the SWCNT-MA if a 3-Tesla magnet is used instead of the 0.3 Tesla used in this study. That means that a SWCNT-MA could be guided 1.5 cm below the skin at least. Today physicists can achieve 17-T fields with cryogenic equipment. However, the intensity of the field proved sufficient for modifying the aggregates orientation and position, and the cells appeared to be strongly attached to the SWCNT-MA.


Dragging human mesenchymal stem cells with the aid of supramolecular assemblies of single-walled carbon nanotubes, molecular magnets, and peptides in a magnetic field.

de Paula AC, Sáfar GA, Góes AM, Bemquerer MP, Ribeiro MA, Stumpf HO - Biomed Res Int (2015)

SWCNT-MA suspension orientation. (a) A suspension of SWCNT-MA in a glass vial. (b) The same suspension with an applied magnet field (originated by the NdFeB magnet at right).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4321095&req=5

fig4: SWCNT-MA suspension orientation. (a) A suspension of SWCNT-MA in a glass vial. (b) The same suspension with an applied magnet field (originated by the NdFeB magnet at right).
Mentions: The SWCNT-MA suspension with and without an applied magnetic field is shown in Figure 4. The aggregation on the vial sidewall where the NdFeB magnet was left shows the ferromagnetism of the SWCNT-MA. To show the practical application of the method, we can make a comparison. For instance, a magnetic field of 3 T is currently used for patients undergoing MRI scans, without disturbing physiological functions [21, 22]. In our experiments, the field never surpassed 0.3 T, which is 10 times lower than that commonly used for MRI. In fact, the magnet used in the study has a 10-fold weaker field than a 3-Tesla magnet used in common MRI equipment. Hence, around a 3-fold larger distance can be expected to have a similar effect on the SWCNT-MA if a 3-Tesla magnet is used instead of the 0.3 Tesla used in this study. That means that a SWCNT-MA could be guided 1.5 cm below the skin at least. Today physicists can achieve 17-T fields with cryogenic equipment. However, the intensity of the field proved sufficient for modifying the aggregates orientation and position, and the cells appeared to be strongly attached to the SWCNT-MA.

Bottom Line: There is a growing number of cell therapies using stem cells due to their characteristics of modulation of immune system and reduction of acute rejection.These assemblies were narrower than their related allotropic forms, that is, multiwalled carbon nanotubes, and they could therefore be used to guide cells through thin blood capillaries within the human body.This strategy seems to be useful as noninvasive and nontoxic stem cells delivery/guidance and tracking during cell therapy.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Bioquímica e Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, 31270-901 Belo Horizonte, MG, Brazil.

ABSTRACT
Human adipose-derived stem cells (hASCs) are an attractive cell source for therapeutic applicability in diverse fields for the repair and regeneration of damaged or malfunctioning tissues and organs. There is a growing number of cell therapies using stem cells due to their characteristics of modulation of immune system and reduction of acute rejection. So a challenge in stem cells therapy is the delivery of cells to the organ of interest, a specific site. The aim of this paper was to investigate the effects of a supramolecular assembly composed of single-walled carbon nanotubes (SWCNT), molecular magnets (lawsone-Co-phenanthroline), and a synthetic peptide (FWYANHYWFHNAFWYANHYWFHNA) in the hASCs cultures. The hASCs were isolated, characterized, expanded, and cultured with the SWCNT supramolecular assembly (SWCNT-MA). The assembly developed did not impair the cell characteristics, viability, or proliferation. During growth, the cells were strongly attached to the assembly and they could be dragged by an applied magnetic field of less than 0.3 T. These assemblies were narrower than their related allotropic forms, that is, multiwalled carbon nanotubes, and they could therefore be used to guide cells through thin blood capillaries within the human body. This strategy seems to be useful as noninvasive and nontoxic stem cells delivery/guidance and tracking during cell therapy.

Show MeSH
Related in: MedlinePlus