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Dragging human mesenchymal stem cells with the aid of supramolecular assemblies of single-walled carbon nanotubes, molecular magnets, and peptides in a magnetic field.

de Paula AC, Sáfar GA, Góes AM, Bemquerer MP, Ribeiro MA, Stumpf HO - Biomed Res Int (2015)

Bottom Line: There is a growing number of cell therapies using stem cells due to their characteristics of modulation of immune system and reduction of acute rejection.These assemblies were narrower than their related allotropic forms, that is, multiwalled carbon nanotubes, and they could therefore be used to guide cells through thin blood capillaries within the human body.This strategy seems to be useful as noninvasive and nontoxic stem cells delivery/guidance and tracking during cell therapy.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Bioquímica e Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, 31270-901 Belo Horizonte, MG, Brazil.

ABSTRACT
Human adipose-derived stem cells (hASCs) are an attractive cell source for therapeutic applicability in diverse fields for the repair and regeneration of damaged or malfunctioning tissues and organs. There is a growing number of cell therapies using stem cells due to their characteristics of modulation of immune system and reduction of acute rejection. So a challenge in stem cells therapy is the delivery of cells to the organ of interest, a specific site. The aim of this paper was to investigate the effects of a supramolecular assembly composed of single-walled carbon nanotubes (SWCNT), molecular magnets (lawsone-Co-phenanthroline), and a synthetic peptide (FWYANHYWFHNAFWYANHYWFHNA) in the hASCs cultures. The hASCs were isolated, characterized, expanded, and cultured with the SWCNT supramolecular assembly (SWCNT-MA). The assembly developed did not impair the cell characteristics, viability, or proliferation. During growth, the cells were strongly attached to the assembly and they could be dragged by an applied magnetic field of less than 0.3 T. These assemblies were narrower than their related allotropic forms, that is, multiwalled carbon nanotubes, and they could therefore be used to guide cells through thin blood capillaries within the human body. This strategy seems to be useful as noninvasive and nontoxic stem cells delivery/guidance and tracking during cell therapy.

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Microscopy image of the stem cells after addition of SWCNT-MA and growth along with their Raman spectra. (a) The red crosses indicate where the RBM Raman mode was found. Bar = 10 μm. (b) Image of the same region after cells were dried. (c) Raman spectra of the red-cross positions in (a). The other characteristic Raman bands, which are related to SWCNT, are also indicated (G, D). (d) The spectra obtained from the blue dots (dried buffer solution). Only a small luminescence appears around 1400 cm−1.
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fig3: Microscopy image of the stem cells after addition of SWCNT-MA and growth along with their Raman spectra. (a) The red crosses indicate where the RBM Raman mode was found. Bar = 10 μm. (b) Image of the same region after cells were dried. (c) Raman spectra of the red-cross positions in (a). The other characteristic Raman bands, which are related to SWCNT, are also indicated (G, D). (d) The spectra obtained from the blue dots (dried buffer solution). Only a small luminescence appears around 1400 cm−1.

Mentions: Figure 3 shows a microscopic view of the cell aggregates, along with their Raman spectra. The red crosses indicate the points where Raman spectra were obtained, showing the RBM characteristic frequency of SWCNT. Spectra were also obtained from the buffer solution (locations indicated by the blue circles), and no traceable SWCNT was found in the buffer solution after 24 hours (Figure 3). The measurements were also obtained in dry aliquots of the buffer solution, where cells were absent, in order to enhance the Raman signal, if there was any signal. The RBM mode was not found in the buffer solution, indicating that SWCNT-MA had indeed adhered to the cell aggregates. Between 10 and 20 cell aggregates were tested for RBM mode signals in each well in order to improve the statistics. Few cells (<5%) were found unattached to any SWCNT. The RBM mode was undetectable at some aggregates which responded to magnetic field. However, the majority (90%) of the aggregates which responded did show the RBM mode signal. Video sequences were recorded and are available online (see Video 1 in Supplementary Material available online at http://dx.doi.org/10.1155/2015/143504).


Dragging human mesenchymal stem cells with the aid of supramolecular assemblies of single-walled carbon nanotubes, molecular magnets, and peptides in a magnetic field.

de Paula AC, Sáfar GA, Góes AM, Bemquerer MP, Ribeiro MA, Stumpf HO - Biomed Res Int (2015)

Microscopy image of the stem cells after addition of SWCNT-MA and growth along with their Raman spectra. (a) The red crosses indicate where the RBM Raman mode was found. Bar = 10 μm. (b) Image of the same region after cells were dried. (c) Raman spectra of the red-cross positions in (a). The other characteristic Raman bands, which are related to SWCNT, are also indicated (G, D). (d) The spectra obtained from the blue dots (dried buffer solution). Only a small luminescence appears around 1400 cm−1.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4321095&req=5

fig3: Microscopy image of the stem cells after addition of SWCNT-MA and growth along with their Raman spectra. (a) The red crosses indicate where the RBM Raman mode was found. Bar = 10 μm. (b) Image of the same region after cells were dried. (c) Raman spectra of the red-cross positions in (a). The other characteristic Raman bands, which are related to SWCNT, are also indicated (G, D). (d) The spectra obtained from the blue dots (dried buffer solution). Only a small luminescence appears around 1400 cm−1.
Mentions: Figure 3 shows a microscopic view of the cell aggregates, along with their Raman spectra. The red crosses indicate the points where Raman spectra were obtained, showing the RBM characteristic frequency of SWCNT. Spectra were also obtained from the buffer solution (locations indicated by the blue circles), and no traceable SWCNT was found in the buffer solution after 24 hours (Figure 3). The measurements were also obtained in dry aliquots of the buffer solution, where cells were absent, in order to enhance the Raman signal, if there was any signal. The RBM mode was not found in the buffer solution, indicating that SWCNT-MA had indeed adhered to the cell aggregates. Between 10 and 20 cell aggregates were tested for RBM mode signals in each well in order to improve the statistics. Few cells (<5%) were found unattached to any SWCNT. The RBM mode was undetectable at some aggregates which responded to magnetic field. However, the majority (90%) of the aggregates which responded did show the RBM mode signal. Video sequences were recorded and are available online (see Video 1 in Supplementary Material available online at http://dx.doi.org/10.1155/2015/143504).

Bottom Line: There is a growing number of cell therapies using stem cells due to their characteristics of modulation of immune system and reduction of acute rejection.These assemblies were narrower than their related allotropic forms, that is, multiwalled carbon nanotubes, and they could therefore be used to guide cells through thin blood capillaries within the human body.This strategy seems to be useful as noninvasive and nontoxic stem cells delivery/guidance and tracking during cell therapy.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Bioquímica e Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, 31270-901 Belo Horizonte, MG, Brazil.

ABSTRACT
Human adipose-derived stem cells (hASCs) are an attractive cell source for therapeutic applicability in diverse fields for the repair and regeneration of damaged or malfunctioning tissues and organs. There is a growing number of cell therapies using stem cells due to their characteristics of modulation of immune system and reduction of acute rejection. So a challenge in stem cells therapy is the delivery of cells to the organ of interest, a specific site. The aim of this paper was to investigate the effects of a supramolecular assembly composed of single-walled carbon nanotubes (SWCNT), molecular magnets (lawsone-Co-phenanthroline), and a synthetic peptide (FWYANHYWFHNAFWYANHYWFHNA) in the hASCs cultures. The hASCs were isolated, characterized, expanded, and cultured with the SWCNT supramolecular assembly (SWCNT-MA). The assembly developed did not impair the cell characteristics, viability, or proliferation. During growth, the cells were strongly attached to the assembly and they could be dragged by an applied magnetic field of less than 0.3 T. These assemblies were narrower than their related allotropic forms, that is, multiwalled carbon nanotubes, and they could therefore be used to guide cells through thin blood capillaries within the human body. This strategy seems to be useful as noninvasive and nontoxic stem cells delivery/guidance and tracking during cell therapy.

Show MeSH
Related in: MedlinePlus