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Dragging human mesenchymal stem cells with the aid of supramolecular assemblies of single-walled carbon nanotubes, molecular magnets, and peptides in a magnetic field.

de Paula AC, Sáfar GA, Góes AM, Bemquerer MP, Ribeiro MA, Stumpf HO - Biomed Res Int (2015)

Bottom Line: There is a growing number of cell therapies using stem cells due to their characteristics of modulation of immune system and reduction of acute rejection.These assemblies were narrower than their related allotropic forms, that is, multiwalled carbon nanotubes, and they could therefore be used to guide cells through thin blood capillaries within the human body.This strategy seems to be useful as noninvasive and nontoxic stem cells delivery/guidance and tracking during cell therapy.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Bioquímica e Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, 31270-901 Belo Horizonte, MG, Brazil.

ABSTRACT
Human adipose-derived stem cells (hASCs) are an attractive cell source for therapeutic applicability in diverse fields for the repair and regeneration of damaged or malfunctioning tissues and organs. There is a growing number of cell therapies using stem cells due to their characteristics of modulation of immune system and reduction of acute rejection. So a challenge in stem cells therapy is the delivery of cells to the organ of interest, a specific site. The aim of this paper was to investigate the effects of a supramolecular assembly composed of single-walled carbon nanotubes (SWCNT), molecular magnets (lawsone-Co-phenanthroline), and a synthetic peptide (FWYANHYWFHNAFWYANHYWFHNA) in the hASCs cultures. The hASCs were isolated, characterized, expanded, and cultured with the SWCNT supramolecular assembly (SWCNT-MA). The assembly developed did not impair the cell characteristics, viability, or proliferation. During growth, the cells were strongly attached to the assembly and they could be dragged by an applied magnetic field of less than 0.3 T. These assemblies were narrower than their related allotropic forms, that is, multiwalled carbon nanotubes, and they could therefore be used to guide cells through thin blood capillaries within the human body. This strategy seems to be useful as noninvasive and nontoxic stem cells delivery/guidance and tracking during cell therapy.

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Related in: MedlinePlus

Human adipose-derived stem cells (hASCs) characterization. (a) Light micrograph of hASCs cultured in basal medium. (b) Cell phenotype analyzed by flow cytometry, expression of the selected mesenchymal stem cell, and hematopoietic and human leukocyte antigens markers are depicted with representative histograms. The white peak indicates the isotype-matched monoclonal antibody control. The black peak indicates positively stained cells. The cell populations expressed CD 29, CD 44, CD 73, and HLA-ABC and did not express CD 45, CD 34, and HLA-DR. (c) Multipotentiality of hASCs. Oil red O staining confirming adipogenesis, Alcian blue staining verifying chondrogenesis and von Kossa staining confirming osteogenesis.
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fig1: Human adipose-derived stem cells (hASCs) characterization. (a) Light micrograph of hASCs cultured in basal medium. (b) Cell phenotype analyzed by flow cytometry, expression of the selected mesenchymal stem cell, and hematopoietic and human leukocyte antigens markers are depicted with representative histograms. The white peak indicates the isotype-matched monoclonal antibody control. The black peak indicates positively stained cells. The cell populations expressed CD 29, CD 44, CD 73, and HLA-ABC and did not express CD 45, CD 34, and HLA-DR. (c) Multipotentiality of hASCs. Oil red O staining confirming adipogenesis, Alcian blue staining verifying chondrogenesis and von Kossa staining confirming osteogenesis.

Mentions: The hASCs isolated from lipoaspirates presented fibroblast-like morphology, plastic-adherent (Figure 1(a)) and expressed CD29 (99.7% ± 0.3%), CD44 (97.3% ± 1.2%), CD73 (99.2 ± 0.5%), and HLA-ABC (99.7% ± 0.02%), and lacked expression of CD45 (9.1% ± 0.0%), CD34 (2.6% ± 0.6%), and HLA-DR (1.04% ± 0.0%) surface molecules (Figure 1(b)). Moreover the hASCs were able to differentiate to adipogenic, chondrogenic, and osteogenic lineages in vitro (Figure 1(c)). These characteristics are in accordance with criteria proposed by the Mesenchymal and Tissue Stem Cell Committee of the International Society for Cellular Therapy to define human MSCs [20].


Dragging human mesenchymal stem cells with the aid of supramolecular assemblies of single-walled carbon nanotubes, molecular magnets, and peptides in a magnetic field.

de Paula AC, Sáfar GA, Góes AM, Bemquerer MP, Ribeiro MA, Stumpf HO - Biomed Res Int (2015)

Human adipose-derived stem cells (hASCs) characterization. (a) Light micrograph of hASCs cultured in basal medium. (b) Cell phenotype analyzed by flow cytometry, expression of the selected mesenchymal stem cell, and hematopoietic and human leukocyte antigens markers are depicted with representative histograms. The white peak indicates the isotype-matched monoclonal antibody control. The black peak indicates positively stained cells. The cell populations expressed CD 29, CD 44, CD 73, and HLA-ABC and did not express CD 45, CD 34, and HLA-DR. (c) Multipotentiality of hASCs. Oil red O staining confirming adipogenesis, Alcian blue staining verifying chondrogenesis and von Kossa staining confirming osteogenesis.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4321095&req=5

fig1: Human adipose-derived stem cells (hASCs) characterization. (a) Light micrograph of hASCs cultured in basal medium. (b) Cell phenotype analyzed by flow cytometry, expression of the selected mesenchymal stem cell, and hematopoietic and human leukocyte antigens markers are depicted with representative histograms. The white peak indicates the isotype-matched monoclonal antibody control. The black peak indicates positively stained cells. The cell populations expressed CD 29, CD 44, CD 73, and HLA-ABC and did not express CD 45, CD 34, and HLA-DR. (c) Multipotentiality of hASCs. Oil red O staining confirming adipogenesis, Alcian blue staining verifying chondrogenesis and von Kossa staining confirming osteogenesis.
Mentions: The hASCs isolated from lipoaspirates presented fibroblast-like morphology, plastic-adherent (Figure 1(a)) and expressed CD29 (99.7% ± 0.3%), CD44 (97.3% ± 1.2%), CD73 (99.2 ± 0.5%), and HLA-ABC (99.7% ± 0.02%), and lacked expression of CD45 (9.1% ± 0.0%), CD34 (2.6% ± 0.6%), and HLA-DR (1.04% ± 0.0%) surface molecules (Figure 1(b)). Moreover the hASCs were able to differentiate to adipogenic, chondrogenic, and osteogenic lineages in vitro (Figure 1(c)). These characteristics are in accordance with criteria proposed by the Mesenchymal and Tissue Stem Cell Committee of the International Society for Cellular Therapy to define human MSCs [20].

Bottom Line: There is a growing number of cell therapies using stem cells due to their characteristics of modulation of immune system and reduction of acute rejection.These assemblies were narrower than their related allotropic forms, that is, multiwalled carbon nanotubes, and they could therefore be used to guide cells through thin blood capillaries within the human body.This strategy seems to be useful as noninvasive and nontoxic stem cells delivery/guidance and tracking during cell therapy.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Bioquímica e Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, 31270-901 Belo Horizonte, MG, Brazil.

ABSTRACT
Human adipose-derived stem cells (hASCs) are an attractive cell source for therapeutic applicability in diverse fields for the repair and regeneration of damaged or malfunctioning tissues and organs. There is a growing number of cell therapies using stem cells due to their characteristics of modulation of immune system and reduction of acute rejection. So a challenge in stem cells therapy is the delivery of cells to the organ of interest, a specific site. The aim of this paper was to investigate the effects of a supramolecular assembly composed of single-walled carbon nanotubes (SWCNT), molecular magnets (lawsone-Co-phenanthroline), and a synthetic peptide (FWYANHYWFHNAFWYANHYWFHNA) in the hASCs cultures. The hASCs were isolated, characterized, expanded, and cultured with the SWCNT supramolecular assembly (SWCNT-MA). The assembly developed did not impair the cell characteristics, viability, or proliferation. During growth, the cells were strongly attached to the assembly and they could be dragged by an applied magnetic field of less than 0.3 T. These assemblies were narrower than their related allotropic forms, that is, multiwalled carbon nanotubes, and they could therefore be used to guide cells through thin blood capillaries within the human body. This strategy seems to be useful as noninvasive and nontoxic stem cells delivery/guidance and tracking during cell therapy.

Show MeSH
Related in: MedlinePlus