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Altered DNA methylation status of human brain derived neurotrophis factor gene could be useful as biomarker of depression.

Song Y, Miyaki K, Suzuki T, Sasaki Y, Tsutsumi A, Kawakami N, Shimazu A, Takahashi M, Inoue A, Kan C, Kurioka S, Shimbo T - Am. J. Med. Genet. B Neuropsychiatr. Genet. (2014)

Bottom Line: The average DNA methylation rate was significantly decreased in the highest K6 score group compared to the lowest group (methylated signals, 14.2% vs. 16.5%, P = 2 · 16 × 10(-198)).Regarding the job-strain score, there was a slight increase in the methylation level of the whole gene in the group with the highest score compared to that with the lowest score; however, these groups showed no difference in the promoter region.Our results revealed significant changes in the DNA methylation status of the complete human BDNF gene in persons with depression compared to normal individuals, especially in the promoter region of exon 1.

View Article: PubMed Central - PubMed

Affiliation: Department of Clinical Research and Informatics, National Center for Global Health and Medicine, Tokyo, Japan.

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Related in: MedlinePlus

Comparison of the DNA methylation status between the groups with the lowest and highest depression levels in (a) the complete gene and (b) the promoter region of exon 1 of the human BDNF gene. The y-axis represents the rate of methylated signals. The group with the lowest depression level (Q1) had the lowest K6 score quartiles, and the group with the highest depression level (Q4) had the highest K6 quartiles.
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fig02: Comparison of the DNA methylation status between the groups with the lowest and highest depression levels in (a) the complete gene and (b) the promoter region of exon 1 of the human BDNF gene. The y-axis represents the rate of methylated signals. The group with the lowest depression level (Q1) had the lowest K6 score quartiles, and the group with the highest depression level (Q4) had the highest K6 quartiles.

Mentions: As shown in Figure 2, the average methylation level of the 97 loci included in the Q4 group was 14.2%, which was significantly lower than that observed in the Q1 group (16.5%, P = 2.16 × 10−198, chi-squared test) (Fig. 2). On the CpG island located in exon 1, the average methylation level for CpGs No. 85–94 of the Q4 group was only half of that for these CpGs of the Q1 group (5.8% and 10.4%, respectively); the difference between the two groups was significant (P = 3.67 × 10−133, chi-squared test).


Altered DNA methylation status of human brain derived neurotrophis factor gene could be useful as biomarker of depression.

Song Y, Miyaki K, Suzuki T, Sasaki Y, Tsutsumi A, Kawakami N, Shimazu A, Takahashi M, Inoue A, Kan C, Kurioka S, Shimbo T - Am. J. Med. Genet. B Neuropsychiatr. Genet. (2014)

Comparison of the DNA methylation status between the groups with the lowest and highest depression levels in (a) the complete gene and (b) the promoter region of exon 1 of the human BDNF gene. The y-axis represents the rate of methylated signals. The group with the lowest depression level (Q1) had the lowest K6 score quartiles, and the group with the highest depression level (Q4) had the highest K6 quartiles.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4321058&req=5

fig02: Comparison of the DNA methylation status between the groups with the lowest and highest depression levels in (a) the complete gene and (b) the promoter region of exon 1 of the human BDNF gene. The y-axis represents the rate of methylated signals. The group with the lowest depression level (Q1) had the lowest K6 score quartiles, and the group with the highest depression level (Q4) had the highest K6 quartiles.
Mentions: As shown in Figure 2, the average methylation level of the 97 loci included in the Q4 group was 14.2%, which was significantly lower than that observed in the Q1 group (16.5%, P = 2.16 × 10−198, chi-squared test) (Fig. 2). On the CpG island located in exon 1, the average methylation level for CpGs No. 85–94 of the Q4 group was only half of that for these CpGs of the Q1 group (5.8% and 10.4%, respectively); the difference between the two groups was significant (P = 3.67 × 10−133, chi-squared test).

Bottom Line: The average DNA methylation rate was significantly decreased in the highest K6 score group compared to the lowest group (methylated signals, 14.2% vs. 16.5%, P = 2 · 16 × 10(-198)).Regarding the job-strain score, there was a slight increase in the methylation level of the whole gene in the group with the highest score compared to that with the lowest score; however, these groups showed no difference in the promoter region.Our results revealed significant changes in the DNA methylation status of the complete human BDNF gene in persons with depression compared to normal individuals, especially in the promoter region of exon 1.

View Article: PubMed Central - PubMed

Affiliation: Department of Clinical Research and Informatics, National Center for Global Health and Medicine, Tokyo, Japan.

Show MeSH
Related in: MedlinePlus