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AtMYB41 activates ectopic suberin synthesis and assembly in multiple plant species and cell types.

Kosma DK, Murmu J, Razeq FM, Santos P, Bourgault R, Molina I, Rowland O - Plant J. (2014)

Bottom Line: Suberin is a lipid and phenolic cell wall heteropolymer found in the roots and other organs of all vascular plants.Overexpression of AtMYB41 also resulted in elevated amounts of monolignols in leaves and an increase in the accumulation of phenylpropanoid and lignin biosynthetic gene transcripts.These results provide insight into the molecular-genetic mechanisms of the biosynthesis and deposition of a ubiquitous cell wall-associated plant structure and will serve as a basis for discovering the transcriptional network behind one of the most abundant lipid-based polymers in nature.

View Article: PubMed Central - PubMed

Affiliation: Department of Plant Biology, Michigan State University, East Lansing, MI, 48824, USA.

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The GUS expression patterns in the primary root of transgenic, 5-day-old Arabidopsis seedlings harboring AtMYB41promoter::GUS (AtMYB41p::GUS) and AtFAR4promoter::GUS (AtFAR4p::GUS) fusions.(a, e) AtMYB41p::GUS untreated. (b, f) AtMYB41p::GUS in the presence of 10 μm abscisic acid (ABA) after 24 h. (c, g) AtMYB41p::GUS in the presence of 200 mm NaCl after 24 h. (d, h) AtFAR4p::GUS untreated. E, epidermis; C, cortex; En, endodermis; v, vascular cylinder. Scale bar: 0.5 cm in (a–d); 100 mm in (e–h).
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fig06: The GUS expression patterns in the primary root of transgenic, 5-day-old Arabidopsis seedlings harboring AtMYB41promoter::GUS (AtMYB41p::GUS) and AtFAR4promoter::GUS (AtFAR4p::GUS) fusions.(a, e) AtMYB41p::GUS untreated. (b, f) AtMYB41p::GUS in the presence of 10 μm abscisic acid (ABA) after 24 h. (c, g) AtMYB41p::GUS in the presence of 200 mm NaCl after 24 h. (d, h) AtFAR4p::GUS untreated. E, epidermis; C, cortex; En, endodermis; v, vascular cylinder. Scale bar: 0.5 cm in (a–d); 100 mm in (e–h).

Mentions: AtMYB41 was previously implicated as playing a role in responses to abiotic stress in an abscisic acid (ABA)-dependent and phosphorylation-dependent manner (via mitogen-activated protein kinase activity) (Cominelli et al., 2008; Lippold et al., 2009; Hoang et al., 2012). Using AtMYB41promoter::GUS transcriptional fusions (AtMYB41p::GUS), we observed that the AtMYB41 promoter drove reporter gene expression in endodermal and surrounding cortical cells under ABA and sodium chloride (NaCl) treatment, but not under unstressed growth conditions (Figure6). Comparison with FAR4promoter::GUS (FAR4p::GUS) lines confirmed endodermal expression. These results demonstrate that the AtMYB41 promoter is active in the endodermis during periods of abiotic stress but not during normal growth.


AtMYB41 activates ectopic suberin synthesis and assembly in multiple plant species and cell types.

Kosma DK, Murmu J, Razeq FM, Santos P, Bourgault R, Molina I, Rowland O - Plant J. (2014)

The GUS expression patterns in the primary root of transgenic, 5-day-old Arabidopsis seedlings harboring AtMYB41promoter::GUS (AtMYB41p::GUS) and AtFAR4promoter::GUS (AtFAR4p::GUS) fusions.(a, e) AtMYB41p::GUS untreated. (b, f) AtMYB41p::GUS in the presence of 10 μm abscisic acid (ABA) after 24 h. (c, g) AtMYB41p::GUS in the presence of 200 mm NaCl after 24 h. (d, h) AtFAR4p::GUS untreated. E, epidermis; C, cortex; En, endodermis; v, vascular cylinder. Scale bar: 0.5 cm in (a–d); 100 mm in (e–h).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4321041&req=5

fig06: The GUS expression patterns in the primary root of transgenic, 5-day-old Arabidopsis seedlings harboring AtMYB41promoter::GUS (AtMYB41p::GUS) and AtFAR4promoter::GUS (AtFAR4p::GUS) fusions.(a, e) AtMYB41p::GUS untreated. (b, f) AtMYB41p::GUS in the presence of 10 μm abscisic acid (ABA) after 24 h. (c, g) AtMYB41p::GUS in the presence of 200 mm NaCl after 24 h. (d, h) AtFAR4p::GUS untreated. E, epidermis; C, cortex; En, endodermis; v, vascular cylinder. Scale bar: 0.5 cm in (a–d); 100 mm in (e–h).
Mentions: AtMYB41 was previously implicated as playing a role in responses to abiotic stress in an abscisic acid (ABA)-dependent and phosphorylation-dependent manner (via mitogen-activated protein kinase activity) (Cominelli et al., 2008; Lippold et al., 2009; Hoang et al., 2012). Using AtMYB41promoter::GUS transcriptional fusions (AtMYB41p::GUS), we observed that the AtMYB41 promoter drove reporter gene expression in endodermal and surrounding cortical cells under ABA and sodium chloride (NaCl) treatment, but not under unstressed growth conditions (Figure6). Comparison with FAR4promoter::GUS (FAR4p::GUS) lines confirmed endodermal expression. These results demonstrate that the AtMYB41 promoter is active in the endodermis during periods of abiotic stress but not during normal growth.

Bottom Line: Suberin is a lipid and phenolic cell wall heteropolymer found in the roots and other organs of all vascular plants.Overexpression of AtMYB41 also resulted in elevated amounts of monolignols in leaves and an increase in the accumulation of phenylpropanoid and lignin biosynthetic gene transcripts.These results provide insight into the molecular-genetic mechanisms of the biosynthesis and deposition of a ubiquitous cell wall-associated plant structure and will serve as a basis for discovering the transcriptional network behind one of the most abundant lipid-based polymers in nature.

View Article: PubMed Central - PubMed

Affiliation: Department of Plant Biology, Michigan State University, East Lansing, MI, 48824, USA.

Show MeSH
Related in: MedlinePlus