Overdominance effect of the bovine ghrelin receptor (GHSR1a)-DelR242 locus on growth in Japanese Shorthorn weaner bulls: heterozygote advantage in bull selection and molecular mechanisms.
Bottom Line: In this study, we found a significant sex difference in the frequency of the 3R allele.Statistical analysis revealed a significant overdominance effect of the DelR242 locus on growth in Japanese Shorthorn weaner bulls.However, additive/dominance/overdominance effects of the 3R allele on carcass traits in adult steers and dams were not significant.
Affiliation: Animal Breeding Research Group, NARO Institute of Livestock and Grassland Science, National Agriculture and Food Research Organization (NARO), Tsukuba, Ibaraki 305-0901, Japan email@example.com.Show MeSH
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Mentions: We analyzed local LD and common haplotype patterns in a 5.8-kb genomic region around the DelR242 locus in GHSR1a. We found a haplotype block of approximately 2 kb from the nt667(C > T) in exon 1 to the nt2884 (A > G) in intron 1 (Figure 1A). In addition, local LD analysis clearly showed that the DelR242 locus is not in LD with nt-7(C > A), the 5′UTR-(TG)n, and the other 27 loci within GHSR1a, except for the nt456(G > A) locus. DelR242 is in LD with the nt456(G > A) locus (r2 = 1) (Figure 1 A). The strong LD between DelR242 and nt456(G > A) was confirmed in 95 sires and 540 half sibs (Figures 1, B and C). It was observed that nt70(C > G), nt580(G > A), nt667(C > T), and intron 1 microsatellite were fixed for the homozygous genotypes [C/C, G/G, C/C, and (GTTT)5/(GTTT)5, respectively] in the 540 half sibs. The 15 markers in intron 1 were considered as fixed for homozygotes on the basis of the result of local LD analysis in Japanese Shorthorn cattle (Figure 1). nt456(G > A) is a synonymous nucleotide substitution in exon 1.
Affiliation: Animal Breeding Research Group, NARO Institute of Livestock and Grassland Science, National Agriculture and Food Research Organization (NARO), Tsukuba, Ibaraki 305-0901, Japan firstname.lastname@example.org.