Limits...
Plant genetic archaeology: whole-genome sequencing reveals the pedigree of a classical trisomic line.

Salomé PA, Weigel D - G3 (Bethesda) (2014)

Bottom Line: The circadian oscillator is astonishingly robust to changes in the environment but also to genomic changes that alter the copy number of its components through genome duplication, gene duplication, and homeologous gene loss.While studying the potential effect of aneuploidy on the Arabidopsis thaliana circadian clock, we discovered that a line thought to be trisomic for chromosome 3 also bears the gi-1 mutation, resulting in a short period and late flowering.With the help of whole-genome sequencing, we uncovered the unexpected complexity of this trisomic stock's history, as its genome shows evidence of past outcrossing with another A. thaliana accession.

View Article: PubMed Central - PubMed

Affiliation: Department of Molecular Biology, Max Planck Institute for Developmental Biology, D-72076 Tübingen, Germany salome@chem.ucla.edu.

Show MeSH
The sequenced CS3227 stock is not trisomic. Normalized read count (over all chromosomes) for Estland/Estonia (Est) Salk (blue line) and the Est-like genomic regions for CS3227 (magenta line).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4321033&req=5

fig7: The sequenced CS3227 stock is not trisomic. Normalized read count (over all chromosomes) for Estland/Estonia (Est) Salk (blue line) and the Est-like genomic regions for CS3227 (magenta line).

Mentions: Finally, we wished to confirm that our CS3227 stock was no longer carrying a third copy of chromosome 3. We therefore plotted normalized read support for all identified Est-like SNPs for CS3227. As shown in Figure 7, read counts for CS3227 were even and showed a pattern very close to that seen in Est from Salk. Importantly, normalized read count for all chromosomes was close to 1, including chromosome 3, indicating that any trisomic history in our stock had been lost prior to the generation we sequenced.


Plant genetic archaeology: whole-genome sequencing reveals the pedigree of a classical trisomic line.

Salomé PA, Weigel D - G3 (Bethesda) (2014)

The sequenced CS3227 stock is not trisomic. Normalized read count (over all chromosomes) for Estland/Estonia (Est) Salk (blue line) and the Est-like genomic regions for CS3227 (magenta line).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4321033&req=5

fig7: The sequenced CS3227 stock is not trisomic. Normalized read count (over all chromosomes) for Estland/Estonia (Est) Salk (blue line) and the Est-like genomic regions for CS3227 (magenta line).
Mentions: Finally, we wished to confirm that our CS3227 stock was no longer carrying a third copy of chromosome 3. We therefore plotted normalized read support for all identified Est-like SNPs for CS3227. As shown in Figure 7, read counts for CS3227 were even and showed a pattern very close to that seen in Est from Salk. Importantly, normalized read count for all chromosomes was close to 1, including chromosome 3, indicating that any trisomic history in our stock had been lost prior to the generation we sequenced.

Bottom Line: The circadian oscillator is astonishingly robust to changes in the environment but also to genomic changes that alter the copy number of its components through genome duplication, gene duplication, and homeologous gene loss.While studying the potential effect of aneuploidy on the Arabidopsis thaliana circadian clock, we discovered that a line thought to be trisomic for chromosome 3 also bears the gi-1 mutation, resulting in a short period and late flowering.With the help of whole-genome sequencing, we uncovered the unexpected complexity of this trisomic stock's history, as its genome shows evidence of past outcrossing with another A. thaliana accession.

View Article: PubMed Central - PubMed

Affiliation: Department of Molecular Biology, Max Planck Institute for Developmental Biology, D-72076 Tübingen, Germany salome@chem.ucla.edu.

Show MeSH