Plant genetic archaeology: whole-genome sequencing reveals the pedigree of a classical trisomic line.
Bottom Line: The circadian oscillator is astonishingly robust to changes in the environment but also to genomic changes that alter the copy number of its components through genome duplication, gene duplication, and homeologous gene loss.While studying the potential effect of aneuploidy on the Arabidopsis thaliana circadian clock, we discovered that a line thought to be trisomic for chromosome 3 also bears the gi-1 mutation, resulting in a short period and late flowering.With the help of whole-genome sequencing, we uncovered the unexpected complexity of this trisomic stock's history, as its genome shows evidence of past outcrossing with another A. thaliana accession.
Affiliation: Department of Molecular Biology, Max Planck Institute for Developmental Biology, D-72076 Tübingen, Germany firstname.lastname@example.org.Show MeSH
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Mentions: In light of the molecular lesion found in CS3227, we re-examined the circadian defects present in CS3227 alongside the weak gi allele gi-1 and the strong alleles gi-2 and gi-201. Both CS3227 and gi-1 displayed a similar short period, while the strong gi alleles did not affect period length significantly (Figure 3). Circadian amplitude of the reporter was markedly decreased in all gi mutants and in CS3227, with strong gi alleles having a stronger effect (with values about 10–14% of wild type, Figure 4. The same reporter was introgressed from Col-2 into all mutant backgrounds, allowing direct comparison of amplitudes). Again, gi-1 and CS3227 behaved in a similar fashion regarding circadian amplitude, which reached about 40% of wild-type values. The CS3227 genetic stock therefore exhibited all circadian defects known to occur in the gi-1 allele.
Affiliation: Department of Molecular Biology, Max Planck Institute for Developmental Biology, D-72076 Tübingen, Germany email@example.com.