Genotyping of endosperms to determine seed dormancy genes regulating germination through embryonic, endospermic, or maternal tissues in rice.
Bottom Line: Many quantitative trait loci (QTL) have been identified for seed dormancy as measured by gross effects on reduced germination rate or delayed germination in crop or model plants.This research developed an endosperm genotype-based genetic approach to determine specific tissues through which a mapped QTL regulates germination using rice as a model.Using this approach, the seed dormancy loci SD12, SD1-2, and SD7-1 were determined to regulate germination through the embryo, endosperm, and maternal tissues, respectively; SD12 and SD1-2 acted additively on germination velocity in the offspring tissues; and SD12 also was associated with the preferential fertilization of male gametes in rice.
Affiliation: Plant Science Department, South Dakota State University, Brookings, South Dakota 57007 Xingyou.email@example.com.Show MeSH
Mentions: Ex. #3 yielded 77% germinated seeds and both germinated and nongerminated seeds were genotyped. Endosperm genotypic frequencies in the two subpopulations fit the expectation (Table 3), which confirmed the observations in Ex. #1 and 2. In addition, the four genotypes of seeds were same in germination distribution pattern (Figure 3A), indicating that they were identical in the degree of seed dormancy. Because SD7-1 had neither endospermic nor embryonic effect on germination in the three experiments, the dormancy gene should express in the maternal tissue(s) during seed development.
Affiliation: Plant Science Department, South Dakota State University, Brookings, South Dakota 57007 Xingyou.firstname.lastname@example.org.