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Salivary desmoglein enzyme-linked immunosorbent assay for diagnosis of pemphigus vulgaris: a noninvasive alternative test to serum assessment.

Mortazavi H, Khatami A, Seyedin Z, Vasheghani Farahani I, Daneshpazhooh M - Biomed Res Int (2015)

Bottom Line: Eighty-six untreated PV patients and 180 age- and sex-matched PV-free controls were recruited in this case-control study.PV was diagnosed based on clinical, histopathological, and direct immunofluorescence findings.The specificity of salivary anti-Dsg 1 and anti-Dsg 3 were both 98.9%.

View Article: PubMed Central - PubMed

Affiliation: Autoimmune Bullous Diseases Research Center, Tehran University of Medical Sciences, Tehran 1199663911, Iran ; Department of Dermatology, Razi Hospital, Tehran University of Medical Sciences, Vahdat Islamic Square, Tehran 1199663911, Iran.

ABSTRACT

Background: Serum desmoglein enzyme-linked immunosorbent assay (ELISA) is used for the diagnosis and monitoring of pemphigus diseases.

Objectives: To compare the diagnostic accuracy of salivary antidesmoglein (Dsg) 1 and 3 ELISA in the diagnosis of pemphigus vulgaris (PV) patients with that of serum desmogleins ELISA.

Methods: Eighty-six untreated PV patients and 180 age- and sex-matched PV-free controls were recruited in this case-control study. PV was diagnosed based on clinical, histopathological, and direct immunofluorescence findings. After processing, serum and salivary anti-Dsg 1 and 3 were measured by the ELISA method using Euroimmun kit (Lübeck, Germany).

Results: Using the cut-off point of 20 relative units (RU)/mL, the serum anti-Dsg 1 and 3 ELISA were positive in 62 (72.1%) and 83 (96.5%) patients, respectively, and the salivary anti-Dsg 1 and 3 ELISA were positive in 31 (36.1%) and 63 (73.3%) patients, respectively. The specificity of salivary anti-Dsg 1 and anti-Dsg 3 were both 98.9%. Optimal cut-off values of 7.7 and 13.4 RU/mL were determined for the salivary anti-Dsg 1 and anti-Dsg 3 ELISA, respectively.

Conclusion: Salivary anti-Dsg 1 and 3 ELISA with high specificities (98.9%) could be suggested as safe and noninvasive methods for the diagnosis of PV when obtaining a blood sample is difficult.

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Related in: MedlinePlus

The ROC curve drawn to obtain the optimal cut-off value for salivary (a) and serum (b) Dsg 1 and 3 ELISA.
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fig1: The ROC curve drawn to obtain the optimal cut-off value for salivary (a) and serum (b) Dsg 1 and 3 ELISA.

Mentions: Using the manufacturer suggested cut-off value of 20 relative units (RU)/mL, the serum anti-Dsg 1 and anti-Dsg 3 ELISA were positive in 62 (72.1%) and 83 (96.5%) of the patients, respectively. The specificities of serum anti-Dsg 1 and anti-Dsg 3 were the same and equaled to 97.8%. We calculated the sensitivity, specificity, +LR, and −LR of serum ELISA with several cut-off points and drew the ROC curve accordingly (Figure 1(b)).


Salivary desmoglein enzyme-linked immunosorbent assay for diagnosis of pemphigus vulgaris: a noninvasive alternative test to serum assessment.

Mortazavi H, Khatami A, Seyedin Z, Vasheghani Farahani I, Daneshpazhooh M - Biomed Res Int (2015)

The ROC curve drawn to obtain the optimal cut-off value for salivary (a) and serum (b) Dsg 1 and 3 ELISA.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4320921&req=5

fig1: The ROC curve drawn to obtain the optimal cut-off value for salivary (a) and serum (b) Dsg 1 and 3 ELISA.
Mentions: Using the manufacturer suggested cut-off value of 20 relative units (RU)/mL, the serum anti-Dsg 1 and anti-Dsg 3 ELISA were positive in 62 (72.1%) and 83 (96.5%) of the patients, respectively. The specificities of serum anti-Dsg 1 and anti-Dsg 3 were the same and equaled to 97.8%. We calculated the sensitivity, specificity, +LR, and −LR of serum ELISA with several cut-off points and drew the ROC curve accordingly (Figure 1(b)).

Bottom Line: Eighty-six untreated PV patients and 180 age- and sex-matched PV-free controls were recruited in this case-control study.PV was diagnosed based on clinical, histopathological, and direct immunofluorescence findings.The specificity of salivary anti-Dsg 1 and anti-Dsg 3 were both 98.9%.

View Article: PubMed Central - PubMed

Affiliation: Autoimmune Bullous Diseases Research Center, Tehran University of Medical Sciences, Tehran 1199663911, Iran ; Department of Dermatology, Razi Hospital, Tehran University of Medical Sciences, Vahdat Islamic Square, Tehran 1199663911, Iran.

ABSTRACT

Background: Serum desmoglein enzyme-linked immunosorbent assay (ELISA) is used for the diagnosis and monitoring of pemphigus diseases.

Objectives: To compare the diagnostic accuracy of salivary antidesmoglein (Dsg) 1 and 3 ELISA in the diagnosis of pemphigus vulgaris (PV) patients with that of serum desmogleins ELISA.

Methods: Eighty-six untreated PV patients and 180 age- and sex-matched PV-free controls were recruited in this case-control study. PV was diagnosed based on clinical, histopathological, and direct immunofluorescence findings. After processing, serum and salivary anti-Dsg 1 and 3 were measured by the ELISA method using Euroimmun kit (Lübeck, Germany).

Results: Using the cut-off point of 20 relative units (RU)/mL, the serum anti-Dsg 1 and 3 ELISA were positive in 62 (72.1%) and 83 (96.5%) patients, respectively, and the salivary anti-Dsg 1 and 3 ELISA were positive in 31 (36.1%) and 63 (73.3%) patients, respectively. The specificity of salivary anti-Dsg 1 and anti-Dsg 3 were both 98.9%. Optimal cut-off values of 7.7 and 13.4 RU/mL were determined for the salivary anti-Dsg 1 and anti-Dsg 3 ELISA, respectively.

Conclusion: Salivary anti-Dsg 1 and 3 ELISA with high specificities (98.9%) could be suggested as safe and noninvasive methods for the diagnosis of PV when obtaining a blood sample is difficult.

Show MeSH
Related in: MedlinePlus