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Serum miR-224 reflects stage of hepatocellular carcinoma and predicts survival.

Zhuang LP, Meng ZQ - Biomed Res Int (2015)

Bottom Line: The serum miR-224 levels were significantly higher in the BCLC stage C patients compared with the stage B patients (P=0.005).Serum levels of miR-224 showed significant relation with parameters of liver damage and serum AFP.It was an independent predictor for the survival of HCC patients.

View Article: PubMed Central - PubMed

Affiliation: Department of Integrative Medicine, Fudan University Shanghai Cancer Center, 270 Dongan Road, Shanghai 200032, China ; Department of Oncology, Shanghai Medical College, Fudan University, Shanghai 200032, China.

ABSTRACT

Background: In our previous study, we conducted a systematic screening of miRNA to identify potential serum biomarkers for predicting venous metastasis and survival in patients with hepatocellular carcinoma (HCC). miR-224 was one of the differentially expressed miRNAs. This study aimed to confirm whether serum miR-224 level is associated with the presence of venous metastasis and survival.

Methods: TaqMan miRNA probe was used to perform qRT-PCR assays to evaluate the expression of serum miR-224 in a cohort of 182 HCC patients.

Results: Patients with high miR-224 serum level showed poor survival compared to that with low miR-224 serum level (HR 1.985; 95% CI, 1.08, 3.65, P=0.027). The serum miR-224 levels were significantly higher in the BCLC stage C patients compared with the stage B patients (P=0.005). In further analysis, significant difference of serum miR-224 expression level was observed when patients grouped by the status of PVTT but not the status of extra-liver metastasis (P=0.013 and P=0.091). Serum levels of miR-224 showed significant relation with parameters of liver damage and serum AFP.

Conclusion: Serum miR-224 might be BCLC stage dependent. It can reflect the status of tumor and liver damage. It was an independent predictor for the survival of HCC patients.

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Serum miR-224 level in different subgroups of patients. (a) A comparison ofmiR-224 expression levels in HCC patients with BCLC B stage and BCLC C stage. Statistical significance was calculated. (b) Serum miR-224 in HCC patients with PVTT was significantly higher than that without PVTT. (c) No significant difference of serum miR-224 level in patients with local advanced HCC and extra-liver metastasis was evident.
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fig2: Serum miR-224 level in different subgroups of patients. (a) A comparison ofmiR-224 expression levels in HCC patients with BCLC B stage and BCLC C stage. Statistical significance was calculated. (b) Serum miR-224 in HCC patients with PVTT was significantly higher than that without PVTT. (c) No significant difference of serum miR-224 level in patients with local advanced HCC and extra-liver metastasis was evident.

Mentions: To examine whether the serum miR-224 levels were associated with specific stages of the disease, the patients were classified according to the BCLC stage. Of the 182 patients with HCC, the serum miR-224 levels were significantly higher in the stage C patients compared with the stage B patients (P = 0.005; Figure 2(a)). In further analysis, we found that the significant difference of serum miR-224 expression level was observed when patients grouped by the status of PVTT but not the status of extra-liver metastasis (P = 0.013 and P = 0.091, Figures 2(b) and 2(c)), suggesting that the serum miR-224 level might be associated with the presence of PVTT. Additionally, we performed Cox proportional hazards regression analysis to exclude the confounder effect. A multivariate analysis confirmed that the serum miR-224 level was an independent prognostic factor for OS (HR 1.985; 95% CI, 1.08, 3.65, P = 0.027) (Table 2).


Serum miR-224 reflects stage of hepatocellular carcinoma and predicts survival.

Zhuang LP, Meng ZQ - Biomed Res Int (2015)

Serum miR-224 level in different subgroups of patients. (a) A comparison ofmiR-224 expression levels in HCC patients with BCLC B stage and BCLC C stage. Statistical significance was calculated. (b) Serum miR-224 in HCC patients with PVTT was significantly higher than that without PVTT. (c) No significant difference of serum miR-224 level in patients with local advanced HCC and extra-liver metastasis was evident.
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Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC4320918&req=5

fig2: Serum miR-224 level in different subgroups of patients. (a) A comparison ofmiR-224 expression levels in HCC patients with BCLC B stage and BCLC C stage. Statistical significance was calculated. (b) Serum miR-224 in HCC patients with PVTT was significantly higher than that without PVTT. (c) No significant difference of serum miR-224 level in patients with local advanced HCC and extra-liver metastasis was evident.
Mentions: To examine whether the serum miR-224 levels were associated with specific stages of the disease, the patients were classified according to the BCLC stage. Of the 182 patients with HCC, the serum miR-224 levels were significantly higher in the stage C patients compared with the stage B patients (P = 0.005; Figure 2(a)). In further analysis, we found that the significant difference of serum miR-224 expression level was observed when patients grouped by the status of PVTT but not the status of extra-liver metastasis (P = 0.013 and P = 0.091, Figures 2(b) and 2(c)), suggesting that the serum miR-224 level might be associated with the presence of PVTT. Additionally, we performed Cox proportional hazards regression analysis to exclude the confounder effect. A multivariate analysis confirmed that the serum miR-224 level was an independent prognostic factor for OS (HR 1.985; 95% CI, 1.08, 3.65, P = 0.027) (Table 2).

Bottom Line: The serum miR-224 levels were significantly higher in the BCLC stage C patients compared with the stage B patients (P=0.005).Serum levels of miR-224 showed significant relation with parameters of liver damage and serum AFP.It was an independent predictor for the survival of HCC patients.

View Article: PubMed Central - PubMed

Affiliation: Department of Integrative Medicine, Fudan University Shanghai Cancer Center, 270 Dongan Road, Shanghai 200032, China ; Department of Oncology, Shanghai Medical College, Fudan University, Shanghai 200032, China.

ABSTRACT

Background: In our previous study, we conducted a systematic screening of miRNA to identify potential serum biomarkers for predicting venous metastasis and survival in patients with hepatocellular carcinoma (HCC). miR-224 was one of the differentially expressed miRNAs. This study aimed to confirm whether serum miR-224 level is associated with the presence of venous metastasis and survival.

Methods: TaqMan miRNA probe was used to perform qRT-PCR assays to evaluate the expression of serum miR-224 in a cohort of 182 HCC patients.

Results: Patients with high miR-224 serum level showed poor survival compared to that with low miR-224 serum level (HR 1.985; 95% CI, 1.08, 3.65, P=0.027). The serum miR-224 levels were significantly higher in the BCLC stage C patients compared with the stage B patients (P=0.005). In further analysis, significant difference of serum miR-224 expression level was observed when patients grouped by the status of PVTT but not the status of extra-liver metastasis (P=0.013 and P=0.091). Serum levels of miR-224 showed significant relation with parameters of liver damage and serum AFP.

Conclusion: Serum miR-224 might be BCLC stage dependent. It can reflect the status of tumor and liver damage. It was an independent predictor for the survival of HCC patients.

Show MeSH
Related in: MedlinePlus