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Isolation and functional characterization of novel biosurfactant produced by Enterococcus faecium.

Sharma D, Saharan BS, Chauhan N, Procha S, Lal S - Springerplus (2015)

Bottom Line: The cell bound BS was partially purified by silica gel column chromatography and found as glycolipid.Current study confirmed the preventive anti-adhesion activity of BS.These amphiphilic molecules, interferes with the microbial adhesion and found to be least cytotoxic with cellular compatibility with mouse fibroblasts cells.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology, Kurukshetra University, Kurukshetra, 136 119 INDIA ; Dairy Microbiology Division, National Dairy Research Institute, Karnal, Haryana 132 001 India.

ABSTRACT
The objective of the present study was to isolate the biosurfactant (BS) producing lactic acid bacteria (LAB) from traditional fermented food (buttermilk) and its functional and structural characterization. BS isolated from strain MRTL9 reduced surface tension from 72.0 to 40.2 mN m(-1). The critical micelle concentration (CMC) of BS was 2.25 mg ml(-1) with emulsification efficiency (E24) after 24 h of 64% against kerosene oil. The cell bound BS was partially purified by silica gel column chromatography and found as glycolipid. The gas chromatography and mass spectroscopy data revealed the fatty acid as hexadecanoic acid. Xylose was determined as hydrophilic moiety. The BS was found to be stable to pH changes over a range of 4.0-12.0, being most effective at pH 7 and showed no apparent loss of surface tension and emulsification efficiency after heat treatment at 120°C for 15 min. The outcomes of cellular toxicity showed lower toxicity of BS in comparison to SDS and rhamnolipids. Current study confirmed the preventive anti-adhesion activity of BS. These amphiphilic molecules, interferes with the microbial adhesion and found to be least cytotoxic with cellular compatibility with mouse fibroblasts cells.

No MeSH data available.


Related in: MedlinePlus

BS produced byE. faeciuminhibits biofilm formation of (A)E. coliATCC 25922 (B)S. aureusATCC 6358P (C)P. aeruginosaATCC 15442 (D)B. cereusATCC 11770 (E)C. albicansMTCC183 (F)L. monocytogenesMTCC1143 on silicone tubes, (-) without BS treatment (+) with BS coating at the concentration of 25 mg ml-1. Biofilm inhibition was visualized by staining with crystal violet.
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Fig9: BS produced byE. faeciuminhibits biofilm formation of (A)E. coliATCC 25922 (B)S. aureusATCC 6358P (C)P. aeruginosaATCC 15442 (D)B. cereusATCC 11770 (E)C. albicansMTCC183 (F)L. monocytogenesMTCC1143 on silicone tubes, (-) without BS treatment (+) with BS coating at the concentration of 25 mg ml-1. Biofilm inhibition was visualized by staining with crystal violet.

Mentions: The biofilm developed by all the pathogenic strains was spread along the whole tube surface (Figure 9). Biofilm formation reduced significantly on silicone tube even at a low concentration of BS (25 mg ml-1). The pre incubation of silicone tubing with known concentration of BS was effective against all biofilm forming pathogens. Moderate anti-biofilm activity was observed against the yeast pathogenic strain C. albicans.Figure 9


Isolation and functional characterization of novel biosurfactant produced by Enterococcus faecium.

Sharma D, Saharan BS, Chauhan N, Procha S, Lal S - Springerplus (2015)

BS produced byE. faeciuminhibits biofilm formation of (A)E. coliATCC 25922 (B)S. aureusATCC 6358P (C)P. aeruginosaATCC 15442 (D)B. cereusATCC 11770 (E)C. albicansMTCC183 (F)L. monocytogenesMTCC1143 on silicone tubes, (-) without BS treatment (+) with BS coating at the concentration of 25 mg ml-1. Biofilm inhibition was visualized by staining with crystal violet.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4320184&req=5

Fig9: BS produced byE. faeciuminhibits biofilm formation of (A)E. coliATCC 25922 (B)S. aureusATCC 6358P (C)P. aeruginosaATCC 15442 (D)B. cereusATCC 11770 (E)C. albicansMTCC183 (F)L. monocytogenesMTCC1143 on silicone tubes, (-) without BS treatment (+) with BS coating at the concentration of 25 mg ml-1. Biofilm inhibition was visualized by staining with crystal violet.
Mentions: The biofilm developed by all the pathogenic strains was spread along the whole tube surface (Figure 9). Biofilm formation reduced significantly on silicone tube even at a low concentration of BS (25 mg ml-1). The pre incubation of silicone tubing with known concentration of BS was effective against all biofilm forming pathogens. Moderate anti-biofilm activity was observed against the yeast pathogenic strain C. albicans.Figure 9

Bottom Line: The cell bound BS was partially purified by silica gel column chromatography and found as glycolipid.Current study confirmed the preventive anti-adhesion activity of BS.These amphiphilic molecules, interferes with the microbial adhesion and found to be least cytotoxic with cellular compatibility with mouse fibroblasts cells.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology, Kurukshetra University, Kurukshetra, 136 119 INDIA ; Dairy Microbiology Division, National Dairy Research Institute, Karnal, Haryana 132 001 India.

ABSTRACT
The objective of the present study was to isolate the biosurfactant (BS) producing lactic acid bacteria (LAB) from traditional fermented food (buttermilk) and its functional and structural characterization. BS isolated from strain MRTL9 reduced surface tension from 72.0 to 40.2 mN m(-1). The critical micelle concentration (CMC) of BS was 2.25 mg ml(-1) with emulsification efficiency (E24) after 24 h of 64% against kerosene oil. The cell bound BS was partially purified by silica gel column chromatography and found as glycolipid. The gas chromatography and mass spectroscopy data revealed the fatty acid as hexadecanoic acid. Xylose was determined as hydrophilic moiety. The BS was found to be stable to pH changes over a range of 4.0-12.0, being most effective at pH 7 and showed no apparent loss of surface tension and emulsification efficiency after heat treatment at 120°C for 15 min. The outcomes of cellular toxicity showed lower toxicity of BS in comparison to SDS and rhamnolipids. Current study confirmed the preventive anti-adhesion activity of BS. These amphiphilic molecules, interferes with the microbial adhesion and found to be least cytotoxic with cellular compatibility with mouse fibroblasts cells.

No MeSH data available.


Related in: MedlinePlus