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Isolation and functional characterization of novel biosurfactant produced by Enterococcus faecium.

Sharma D, Saharan BS, Chauhan N, Procha S, Lal S - Springerplus (2015)

Bottom Line: The cell bound BS was partially purified by silica gel column chromatography and found as glycolipid.Current study confirmed the preventive anti-adhesion activity of BS.These amphiphilic molecules, interferes with the microbial adhesion and found to be least cytotoxic with cellular compatibility with mouse fibroblasts cells.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology, Kurukshetra University, Kurukshetra, 136 119 INDIA ; Dairy Microbiology Division, National Dairy Research Institute, Karnal, Haryana 132 001 India.

ABSTRACT
The objective of the present study was to isolate the biosurfactant (BS) producing lactic acid bacteria (LAB) from traditional fermented food (buttermilk) and its functional and structural characterization. BS isolated from strain MRTL9 reduced surface tension from 72.0 to 40.2 mN m(-1). The critical micelle concentration (CMC) of BS was 2.25 mg ml(-1) with emulsification efficiency (E24) after 24 h of 64% against kerosene oil. The cell bound BS was partially purified by silica gel column chromatography and found as glycolipid. The gas chromatography and mass spectroscopy data revealed the fatty acid as hexadecanoic acid. Xylose was determined as hydrophilic moiety. The BS was found to be stable to pH changes over a range of 4.0-12.0, being most effective at pH 7 and showed no apparent loss of surface tension and emulsification efficiency after heat treatment at 120°C for 15 min. The outcomes of cellular toxicity showed lower toxicity of BS in comparison to SDS and rhamnolipids. Current study confirmed the preventive anti-adhesion activity of BS. These amphiphilic molecules, interferes with the microbial adhesion and found to be least cytotoxic with cellular compatibility with mouse fibroblasts cells.

No MeSH data available.


Related in: MedlinePlus

Effect of pH on the surface tension of the cell-bound partially purified BS isolated fromE. faeciumMRTL9.
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Fig7: Effect of pH on the surface tension of the cell-bound partially purified BS isolated fromE. faeciumMRTL9.

Mentions: The application of BS is depends on their stability at extreme temperatures and pH range. The stability of BS was studied at different pH, temperature and observed in conviction of effect on ST and emulsification index. Figures 7 and 8, present the effects of temperature and pH on surface tension and emulsification capacity of cell bound BS. In current study, partially purified BS retained activity over pH range from 6 to 10 with a minimal deviation in surface tension values. The pH values below and upper 7 showed minor increase in surface tension but found to be stable, the BS sustained activity even after treatment at 0–120°C for 30 min. The effect of different pH and incubation at extreme temperature on surface tension and emulsification capacity were insignificant. Heat treatment (autoclaving at 120°C for 15 min) on BS caused no appreciable changes in their surface and emulsifying activities (Desai and Banat 1997). Biosurfactant produced by Pseudomonas aeruginosa SP4 was also found stable isolated from petroleum contaminated soil (Pornsunthorntawee et al. 2008). BS from Candida lipolytica cultivated with industrial residue and a lipopeptides from Bacillus subtilis C9 cultivated with a carbohydrate substrate demonstrated similar stability behavior (Rufino et al. 2008). Lipopeptides of Bacillus subtilis also reported with stability at elevated temperature and different range of pH (Vaz et al. 2012). The BS was structurally stable and retained its surface activity even at the extreme pH and high temperature range. Stability of BS at high temperature also conferred in accordance with the results obtained after TG analysis.Figure 7


Isolation and functional characterization of novel biosurfactant produced by Enterococcus faecium.

Sharma D, Saharan BS, Chauhan N, Procha S, Lal S - Springerplus (2015)

Effect of pH on the surface tension of the cell-bound partially purified BS isolated fromE. faeciumMRTL9.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4320184&req=5

Fig7: Effect of pH on the surface tension of the cell-bound partially purified BS isolated fromE. faeciumMRTL9.
Mentions: The application of BS is depends on their stability at extreme temperatures and pH range. The stability of BS was studied at different pH, temperature and observed in conviction of effect on ST and emulsification index. Figures 7 and 8, present the effects of temperature and pH on surface tension and emulsification capacity of cell bound BS. In current study, partially purified BS retained activity over pH range from 6 to 10 with a minimal deviation in surface tension values. The pH values below and upper 7 showed minor increase in surface tension but found to be stable, the BS sustained activity even after treatment at 0–120°C for 30 min. The effect of different pH and incubation at extreme temperature on surface tension and emulsification capacity were insignificant. Heat treatment (autoclaving at 120°C for 15 min) on BS caused no appreciable changes in their surface and emulsifying activities (Desai and Banat 1997). Biosurfactant produced by Pseudomonas aeruginosa SP4 was also found stable isolated from petroleum contaminated soil (Pornsunthorntawee et al. 2008). BS from Candida lipolytica cultivated with industrial residue and a lipopeptides from Bacillus subtilis C9 cultivated with a carbohydrate substrate demonstrated similar stability behavior (Rufino et al. 2008). Lipopeptides of Bacillus subtilis also reported with stability at elevated temperature and different range of pH (Vaz et al. 2012). The BS was structurally stable and retained its surface activity even at the extreme pH and high temperature range. Stability of BS at high temperature also conferred in accordance with the results obtained after TG analysis.Figure 7

Bottom Line: The cell bound BS was partially purified by silica gel column chromatography and found as glycolipid.Current study confirmed the preventive anti-adhesion activity of BS.These amphiphilic molecules, interferes with the microbial adhesion and found to be least cytotoxic with cellular compatibility with mouse fibroblasts cells.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology, Kurukshetra University, Kurukshetra, 136 119 INDIA ; Dairy Microbiology Division, National Dairy Research Institute, Karnal, Haryana 132 001 India.

ABSTRACT
The objective of the present study was to isolate the biosurfactant (BS) producing lactic acid bacteria (LAB) from traditional fermented food (buttermilk) and its functional and structural characterization. BS isolated from strain MRTL9 reduced surface tension from 72.0 to 40.2 mN m(-1). The critical micelle concentration (CMC) of BS was 2.25 mg ml(-1) with emulsification efficiency (E24) after 24 h of 64% against kerosene oil. The cell bound BS was partially purified by silica gel column chromatography and found as glycolipid. The gas chromatography and mass spectroscopy data revealed the fatty acid as hexadecanoic acid. Xylose was determined as hydrophilic moiety. The BS was found to be stable to pH changes over a range of 4.0-12.0, being most effective at pH 7 and showed no apparent loss of surface tension and emulsification efficiency after heat treatment at 120°C for 15 min. The outcomes of cellular toxicity showed lower toxicity of BS in comparison to SDS and rhamnolipids. Current study confirmed the preventive anti-adhesion activity of BS. These amphiphilic molecules, interferes with the microbial adhesion and found to be least cytotoxic with cellular compatibility with mouse fibroblasts cells.

No MeSH data available.


Related in: MedlinePlus