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Isolation and functional characterization of novel biosurfactant produced by Enterococcus faecium.

Sharma D, Saharan BS, Chauhan N, Procha S, Lal S - Springerplus (2015)

Bottom Line: The cell bound BS was partially purified by silica gel column chromatography and found as glycolipid.Current study confirmed the preventive anti-adhesion activity of BS.These amphiphilic molecules, interferes with the microbial adhesion and found to be least cytotoxic with cellular compatibility with mouse fibroblasts cells.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology, Kurukshetra University, Kurukshetra, 136 119 INDIA ; Dairy Microbiology Division, National Dairy Research Institute, Karnal, Haryana 132 001 India.

ABSTRACT
The objective of the present study was to isolate the biosurfactant (BS) producing lactic acid bacteria (LAB) from traditional fermented food (buttermilk) and its functional and structural characterization. BS isolated from strain MRTL9 reduced surface tension from 72.0 to 40.2 mN m(-1). The critical micelle concentration (CMC) of BS was 2.25 mg ml(-1) with emulsification efficiency (E24) after 24 h of 64% against kerosene oil. The cell bound BS was partially purified by silica gel column chromatography and found as glycolipid. The gas chromatography and mass spectroscopy data revealed the fatty acid as hexadecanoic acid. Xylose was determined as hydrophilic moiety. The BS was found to be stable to pH changes over a range of 4.0-12.0, being most effective at pH 7 and showed no apparent loss of surface tension and emulsification efficiency after heat treatment at 120°C for 15 min. The outcomes of cellular toxicity showed lower toxicity of BS in comparison to SDS and rhamnolipids. Current study confirmed the preventive anti-adhesion activity of BS. These amphiphilic molecules, interferes with the microbial adhesion and found to be least cytotoxic with cellular compatibility with mouse fibroblasts cells.

No MeSH data available.


Related in: MedlinePlus

Decrease of surface tension with time by cell bound BS.
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Fig1: Decrease of surface tension with time by cell bound BS.

Mentions: The production of BS was carried out by inoculating with 1% overnight pre culture at 37°C in a rotary shaker for 72 h. After incubation, ST of the cell bound BS in PBS reduced to 40.2 mN m-1 from 72 mN m-1. ST of the production media was reduced with the course of incubation time (Figure 1). The significant decrease of ST established the production of BS by the E. faecium MRTL 9. Maximal ST reduction was observed at late logarithmic and early stationary phase of cell growth. Reduction in ST during the logarithmic and stationary phase has been reported and confirmed the production of BS (Oliveira and Garcia-Cruz 2013; Madhu and Prapulla 2014). BS was extracted twice with equal volume of ethyl acetate; crude BS (brown in color) was obtained and partially purified using column chromatography (Silica gel; 60–120 mesh size). Fraction with highest activity were pooled and analyzed by different thin layer chromatography (TLC) with post chromatographic detections.Figure 1


Isolation and functional characterization of novel biosurfactant produced by Enterococcus faecium.

Sharma D, Saharan BS, Chauhan N, Procha S, Lal S - Springerplus (2015)

Decrease of surface tension with time by cell bound BS.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4320184&req=5

Fig1: Decrease of surface tension with time by cell bound BS.
Mentions: The production of BS was carried out by inoculating with 1% overnight pre culture at 37°C in a rotary shaker for 72 h. After incubation, ST of the cell bound BS in PBS reduced to 40.2 mN m-1 from 72 mN m-1. ST of the production media was reduced with the course of incubation time (Figure 1). The significant decrease of ST established the production of BS by the E. faecium MRTL 9. Maximal ST reduction was observed at late logarithmic and early stationary phase of cell growth. Reduction in ST during the logarithmic and stationary phase has been reported and confirmed the production of BS (Oliveira and Garcia-Cruz 2013; Madhu and Prapulla 2014). BS was extracted twice with equal volume of ethyl acetate; crude BS (brown in color) was obtained and partially purified using column chromatography (Silica gel; 60–120 mesh size). Fraction with highest activity were pooled and analyzed by different thin layer chromatography (TLC) with post chromatographic detections.Figure 1

Bottom Line: The cell bound BS was partially purified by silica gel column chromatography and found as glycolipid.Current study confirmed the preventive anti-adhesion activity of BS.These amphiphilic molecules, interferes with the microbial adhesion and found to be least cytotoxic with cellular compatibility with mouse fibroblasts cells.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology, Kurukshetra University, Kurukshetra, 136 119 INDIA ; Dairy Microbiology Division, National Dairy Research Institute, Karnal, Haryana 132 001 India.

ABSTRACT
The objective of the present study was to isolate the biosurfactant (BS) producing lactic acid bacteria (LAB) from traditional fermented food (buttermilk) and its functional and structural characterization. BS isolated from strain MRTL9 reduced surface tension from 72.0 to 40.2 mN m(-1). The critical micelle concentration (CMC) of BS was 2.25 mg ml(-1) with emulsification efficiency (E24) after 24 h of 64% against kerosene oil. The cell bound BS was partially purified by silica gel column chromatography and found as glycolipid. The gas chromatography and mass spectroscopy data revealed the fatty acid as hexadecanoic acid. Xylose was determined as hydrophilic moiety. The BS was found to be stable to pH changes over a range of 4.0-12.0, being most effective at pH 7 and showed no apparent loss of surface tension and emulsification efficiency after heat treatment at 120°C for 15 min. The outcomes of cellular toxicity showed lower toxicity of BS in comparison to SDS and rhamnolipids. Current study confirmed the preventive anti-adhesion activity of BS. These amphiphilic molecules, interferes with the microbial adhesion and found to be least cytotoxic with cellular compatibility with mouse fibroblasts cells.

No MeSH data available.


Related in: MedlinePlus