Limits...
Statistically optimized biotransformation protocol for continuous production of L-DOPA using Mucuna monosperma callus culture.

Inamdar SA, Surwase SN, Jadhav SB, Bapat VA, Jadhav JP - Springerplus (2013)

Bottom Line: The optimization of medium using RSM resulted in a 3.45-fold increase in the yield of L-DOPA.The ANOVA analysis showed a significant R (2) value (0.9912), model F-value (112.465) and probability (0.0001), with insignificant lack of fit.L-DOPA production was confirmed by HPTLC and HPLC analysis.

View Article: PubMed Central - PubMed

Affiliation: Department of Biotechnology, Shivaji University, Kolhapur, 416 004 India.

ABSTRACT
L-DOPA (3,4-dihydroxyphenyl-L-alanine), a modified amino acid, is an expansively used drug for the Parkinson's disease treatment. In the present study, optimization of nutritional parameters influencing L-DOPA production was attempted using the response surface methodology (RSM) from Mucuna monosperma callus. Optimization of the four factors was carried out using the Box-Behnken design. The optimized levels of factors predicted by the model include tyrosine 0.894 g l(-1), pH 4.99, ascorbic acid 31.62 mg l(-1)and copper sulphate 23.92 mg l(-1), which resulted in highest L-DOPA yield of 0.309 g l(-1). The optimization of medium using RSM resulted in a 3.45-fold increase in the yield of L-DOPA. The ANOVA analysis showed a significant R (2) value (0.9912), model F-value (112.465) and probability (0.0001), with insignificant lack of fit. Optimized medium was used in the laboratory scale column reactor for continuous production of L-DOPA. Uninterrupted flow column exhibited maximum L-DOPA production rate of 200 mg L(-1) h(-1) which is one of the highest values ever reported using plant as a biotransformation source. L-DOPA production was confirmed by HPTLC and HPLC analysis. This study demonstrates the synthesis of L- DOPA using Mucuna monosperma callus using a laboratory scale column reactor.

No MeSH data available.


Related in: MedlinePlus

Schematic diagram of laboratory scale column reactor.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4320180&req=5

Fig4: Schematic diagram of laboratory scale column reactor.

Mentions: Continuous production of L-DOPA was achieved using a laboratory scale column (3 cm Φ × 30 cm) with a provision for aeration at the bottom. Medium was allowed to pass through bottom of the column using peristaltic pump and produced L-DOPA was recovered from the top of the column (Figure 4). Sterile air was introduced in to the column using aerator and bacteria proof filter. Flow rate was controlled using peristaltic pump.Figure 4


Statistically optimized biotransformation protocol for continuous production of L-DOPA using Mucuna monosperma callus culture.

Inamdar SA, Surwase SN, Jadhav SB, Bapat VA, Jadhav JP - Springerplus (2013)

Schematic diagram of laboratory scale column reactor.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4320180&req=5

Fig4: Schematic diagram of laboratory scale column reactor.
Mentions: Continuous production of L-DOPA was achieved using a laboratory scale column (3 cm Φ × 30 cm) with a provision for aeration at the bottom. Medium was allowed to pass through bottom of the column using peristaltic pump and produced L-DOPA was recovered from the top of the column (Figure 4). Sterile air was introduced in to the column using aerator and bacteria proof filter. Flow rate was controlled using peristaltic pump.Figure 4

Bottom Line: The optimization of medium using RSM resulted in a 3.45-fold increase in the yield of L-DOPA.The ANOVA analysis showed a significant R (2) value (0.9912), model F-value (112.465) and probability (0.0001), with insignificant lack of fit.L-DOPA production was confirmed by HPTLC and HPLC analysis.

View Article: PubMed Central - PubMed

Affiliation: Department of Biotechnology, Shivaji University, Kolhapur, 416 004 India.

ABSTRACT
L-DOPA (3,4-dihydroxyphenyl-L-alanine), a modified amino acid, is an expansively used drug for the Parkinson's disease treatment. In the present study, optimization of nutritional parameters influencing L-DOPA production was attempted using the response surface methodology (RSM) from Mucuna monosperma callus. Optimization of the four factors was carried out using the Box-Behnken design. The optimized levels of factors predicted by the model include tyrosine 0.894 g l(-1), pH 4.99, ascorbic acid 31.62 mg l(-1)and copper sulphate 23.92 mg l(-1), which resulted in highest L-DOPA yield of 0.309 g l(-1). The optimization of medium using RSM resulted in a 3.45-fold increase in the yield of L-DOPA. The ANOVA analysis showed a significant R (2) value (0.9912), model F-value (112.465) and probability (0.0001), with insignificant lack of fit. Optimized medium was used in the laboratory scale column reactor for continuous production of L-DOPA. Uninterrupted flow column exhibited maximum L-DOPA production rate of 200 mg L(-1) h(-1) which is one of the highest values ever reported using plant as a biotransformation source. L-DOPA production was confirmed by HPTLC and HPLC analysis. This study demonstrates the synthesis of L- DOPA using Mucuna monosperma callus using a laboratory scale column reactor.

No MeSH data available.


Related in: MedlinePlus