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Suicin 3908, a new lantibiotic produced by a strain of Streptococcus suis serotype 2 isolated from a healthy carrier pig.

Vaillancourt K, LeBel G, Frenette M, Gottschalk M, Grenier D - PLoS ONE (2015)

Bottom Line: Additive effects were obtained when suicin 3908 was used in combination with penicillin G or amoxicillin.The putative gene cluster involved in suicin production by S. suis 3908 was amplified by PCR, and the sequence analysis revealed the presence of nine open reading frames (ORFs), including the structural gene and those required for the modification of amino acids, export, regulation, and immunity.Suicin 3908, which is encoded by the suiA gene, exhibited approximately 50% identity with bovicin HJ50 (Streptococcus bovis), thermophilin 1277 (Streptococcus thermophilus), and macedovicin (Streptococcus macedonicus).

View Article: PubMed Central - PubMed

Affiliation: Groupe de Recherche en Écologie Buccale (GREB), Faculté de Médecine Dentaire, Université Laval, Quebec City, QC, Canada.

ABSTRACT
While Streptococcus suis serotype 2 is known to cause severe infections in pigs, it can also be isolated from the tonsils of healthy animals that do not develop infections. We hypothesized that S. suis strains in healthy carrier pigs may have the ability to produce bacteriocins, which may contribute to preventing infections by pathogenic S. suis strains. Two of ten S. suis serotype 2 strains isolated from healthy carrier pigs exhibited antibacterial activity against pathogenic S. suis isolates. The bacteriocin produced by S. suis 3908 was purified to homogeneity using a three-step procedure: ammonium sulfate precipitation, cationic exchange HPLC, and reversed-phase HPLC. The bacteriocin, called suicin 3908, had a low molecular mass; was resistant to heat, pH, and protease treatments; and possessed membrane permeabilization activity. Additive effects were obtained when suicin 3908 was used in combination with penicillin G or amoxicillin. The amino acid sequence of suicin 3908 suggested that it is lantibiotic-related and made it possible to identify a bacteriocin locus in the genome of S. suis D12. The putative gene cluster involved in suicin production by S. suis 3908 was amplified by PCR, and the sequence analysis revealed the presence of nine open reading frames (ORFs), including the structural gene and those required for the modification of amino acids, export, regulation, and immunity. Suicin 3908, which is encoded by the suiA gene, exhibited approximately 50% identity with bovicin HJ50 (Streptococcus bovis), thermophilin 1277 (Streptococcus thermophilus), and macedovicin (Streptococcus macedonicus). Given that S. suis 3908 cannot cause infections in animal models, that it is susceptible to conventional antibiotics, and that it produces a bacteriocin with antibacterial activity against all pathogenic S. suis strains tested, it could potentially be used to prevent infections and to reduce antibiotic use by the swine industry.

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Effect of suicin 3908 on the membrane permeabilization of S. suis MGGUS3 as determined using the SYTOX Green dye.Ethanol (70%) was used as a positive control. Three assays were performed. The results are expressed as means ± standard deviations.
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pone.0117245.g004: Effect of suicin 3908 on the membrane permeabilization of S. suis MGGUS3 as determined using the SYTOX Green dye.Ethanol (70%) was used as a positive control. Three assays were performed. The results are expressed as means ± standard deviations.

Mentions: The ability of suicin 3908 to permeabilize the membrane of the indicator strain S. suis MGGUS3 was investigated using SYTOX Green dye, which can penetrate damaged cytoplasmic membranes and react with DNA. As shown in Fig. 4, the addition of either the purified bacteriocin or ethanol (positive control) resulted in a time-dependent increase in fluorescence, indicating that the membrane of S. suis MGGUS3 was permeabilized by suicin 3908. No increase in fluorescence occurred with the negative control.


Suicin 3908, a new lantibiotic produced by a strain of Streptococcus suis serotype 2 isolated from a healthy carrier pig.

Vaillancourt K, LeBel G, Frenette M, Gottschalk M, Grenier D - PLoS ONE (2015)

Effect of suicin 3908 on the membrane permeabilization of S. suis MGGUS3 as determined using the SYTOX Green dye.Ethanol (70%) was used as a positive control. Three assays were performed. The results are expressed as means ± standard deviations.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4320106&req=5

pone.0117245.g004: Effect of suicin 3908 on the membrane permeabilization of S. suis MGGUS3 as determined using the SYTOX Green dye.Ethanol (70%) was used as a positive control. Three assays were performed. The results are expressed as means ± standard deviations.
Mentions: The ability of suicin 3908 to permeabilize the membrane of the indicator strain S. suis MGGUS3 was investigated using SYTOX Green dye, which can penetrate damaged cytoplasmic membranes and react with DNA. As shown in Fig. 4, the addition of either the purified bacteriocin or ethanol (positive control) resulted in a time-dependent increase in fluorescence, indicating that the membrane of S. suis MGGUS3 was permeabilized by suicin 3908. No increase in fluorescence occurred with the negative control.

Bottom Line: Additive effects were obtained when suicin 3908 was used in combination with penicillin G or amoxicillin.The putative gene cluster involved in suicin production by S. suis 3908 was amplified by PCR, and the sequence analysis revealed the presence of nine open reading frames (ORFs), including the structural gene and those required for the modification of amino acids, export, regulation, and immunity.Suicin 3908, which is encoded by the suiA gene, exhibited approximately 50% identity with bovicin HJ50 (Streptococcus bovis), thermophilin 1277 (Streptococcus thermophilus), and macedovicin (Streptococcus macedonicus).

View Article: PubMed Central - PubMed

Affiliation: Groupe de Recherche en Écologie Buccale (GREB), Faculté de Médecine Dentaire, Université Laval, Quebec City, QC, Canada.

ABSTRACT
While Streptococcus suis serotype 2 is known to cause severe infections in pigs, it can also be isolated from the tonsils of healthy animals that do not develop infections. We hypothesized that S. suis strains in healthy carrier pigs may have the ability to produce bacteriocins, which may contribute to preventing infections by pathogenic S. suis strains. Two of ten S. suis serotype 2 strains isolated from healthy carrier pigs exhibited antibacterial activity against pathogenic S. suis isolates. The bacteriocin produced by S. suis 3908 was purified to homogeneity using a three-step procedure: ammonium sulfate precipitation, cationic exchange HPLC, and reversed-phase HPLC. The bacteriocin, called suicin 3908, had a low molecular mass; was resistant to heat, pH, and protease treatments; and possessed membrane permeabilization activity. Additive effects were obtained when suicin 3908 was used in combination with penicillin G or amoxicillin. The amino acid sequence of suicin 3908 suggested that it is lantibiotic-related and made it possible to identify a bacteriocin locus in the genome of S. suis D12. The putative gene cluster involved in suicin production by S. suis 3908 was amplified by PCR, and the sequence analysis revealed the presence of nine open reading frames (ORFs), including the structural gene and those required for the modification of amino acids, export, regulation, and immunity. Suicin 3908, which is encoded by the suiA gene, exhibited approximately 50% identity with bovicin HJ50 (Streptococcus bovis), thermophilin 1277 (Streptococcus thermophilus), and macedovicin (Streptococcus macedonicus). Given that S. suis 3908 cannot cause infections in animal models, that it is susceptible to conventional antibiotics, and that it produces a bacteriocin with antibacterial activity against all pathogenic S. suis strains tested, it could potentially be used to prevent infections and to reduce antibiotic use by the swine industry.

Show MeSH
Related in: MedlinePlus