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Cross-talk between human neural stem/progenitor cells and peripheral blood mononuclear cells in an allogeneic co-culture model.

Zhang H, Shao B, Zhuge Q, Wang P, Zheng C, Huang W, Yang C, Wang B, Su DM, Jin K - PLoS ONE (2015)

Bottom Line: Transplantation of human neural stem/progenitor cells (hNSCs) as a regenerative cell replacement therapy holds great promise.However, the underlying mechanisms remain unclear.We found that hNSCs significantly decrease the CD3+ and CD8+ T cells, reduce the gamma delta T cells and increase the regulatory T cells, along with reduced pro-inflammatory cytokines and increased anti-inflammatory cytokines after co-culture.

View Article: PubMed Central - PubMed

Affiliation: Zhejiang Provincial Key Laboratory of Aging and Neurological Disorder Research, First Affiliated Hospital, Wenzhou Medical University, Wenzhou 35000, China.

ABSTRACT
Transplantation of human neural stem/progenitor cells (hNSCs) as a regenerative cell replacement therapy holds great promise. However, the underlying mechanisms remain unclear. We, here, focused on the interaction between hNSCs and allogeneic peripheral blood mononuclear cells (PBMCs) in a co-culture model. We found that hNSCs significantly decrease the CD3+ and CD8+ T cells, reduce the gamma delta T cells and increase the regulatory T cells, along with reduced pro-inflammatory cytokines and increased anti-inflammatory cytokines after co-culture. We also found that PBMCs, in turn, significantly promote the proliferation and differentiation of hNSCs. Our data suggest that hNSCs cross-talk with immune cells.

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Effects of PBMCs (5:1) on apoptosis and proliferation of NSCs.(a). Representative dot plots of T cell proportion (CD3+). (b). Representative dot plots of apoptotic NSCs (Annexin V and PI). (c). Statistical analysis of necrotic and apoptotic cells. (d). Representative histogram of proliferation of NSCs (CFSE). (e). Statistical analysis of proliferation of NSCs (CFSE). (f). Statistical analysis of proliferation of NSCs (CCK-8). *, P < 0.05. The experiments were repeated at least 3 times for each experiment.
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pone.0117432.g006: Effects of PBMCs (5:1) on apoptosis and proliferation of NSCs.(a). Representative dot plots of T cell proportion (CD3+). (b). Representative dot plots of apoptotic NSCs (Annexin V and PI). (c). Statistical analysis of necrotic and apoptotic cells. (d). Representative histogram of proliferation of NSCs (CFSE). (e). Statistical analysis of proliferation of NSCs (CFSE). (f). Statistical analysis of proliferation of NSCs (CCK-8). *, P < 0.05. The experiments were repeated at least 3 times for each experiment.

Mentions: To assess the apoptosis or death of hNSCs, hNSCs with Annexin/V and PI were stained after co-culture at a ratio of 5:1 for 48 hrs. We found no significant differences between the two groups (Fig. 6A-C). Next, we determined whether the proliferation of hNSCs was influenced by PBMCs. PBMCs were co-cultured with CFSE-labelled hNSCs in 24-well plate for 48 hrs and the presence of PBMCs apparently stimulated the proliferation of hNSCs (Fig. 6D-E). Consistent with the CFSE result, the evaluation of CCK-8 in a transwell culture system also showed there was a notable proliferation of hNSCs when hNSCs were co-cultured with PBMCs, compared to control group (Fig. 6F).


Cross-talk between human neural stem/progenitor cells and peripheral blood mononuclear cells in an allogeneic co-culture model.

Zhang H, Shao B, Zhuge Q, Wang P, Zheng C, Huang W, Yang C, Wang B, Su DM, Jin K - PLoS ONE (2015)

Effects of PBMCs (5:1) on apoptosis and proliferation of NSCs.(a). Representative dot plots of T cell proportion (CD3+). (b). Representative dot plots of apoptotic NSCs (Annexin V and PI). (c). Statistical analysis of necrotic and apoptotic cells. (d). Representative histogram of proliferation of NSCs (CFSE). (e). Statistical analysis of proliferation of NSCs (CFSE). (f). Statistical analysis of proliferation of NSCs (CCK-8). *, P < 0.05. The experiments were repeated at least 3 times for each experiment.
© Copyright Policy
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC4319716&req=5

pone.0117432.g006: Effects of PBMCs (5:1) on apoptosis and proliferation of NSCs.(a). Representative dot plots of T cell proportion (CD3+). (b). Representative dot plots of apoptotic NSCs (Annexin V and PI). (c). Statistical analysis of necrotic and apoptotic cells. (d). Representative histogram of proliferation of NSCs (CFSE). (e). Statistical analysis of proliferation of NSCs (CFSE). (f). Statistical analysis of proliferation of NSCs (CCK-8). *, P < 0.05. The experiments were repeated at least 3 times for each experiment.
Mentions: To assess the apoptosis or death of hNSCs, hNSCs with Annexin/V and PI were stained after co-culture at a ratio of 5:1 for 48 hrs. We found no significant differences between the two groups (Fig. 6A-C). Next, we determined whether the proliferation of hNSCs was influenced by PBMCs. PBMCs were co-cultured with CFSE-labelled hNSCs in 24-well plate for 48 hrs and the presence of PBMCs apparently stimulated the proliferation of hNSCs (Fig. 6D-E). Consistent with the CFSE result, the evaluation of CCK-8 in a transwell culture system also showed there was a notable proliferation of hNSCs when hNSCs were co-cultured with PBMCs, compared to control group (Fig. 6F).

Bottom Line: Transplantation of human neural stem/progenitor cells (hNSCs) as a regenerative cell replacement therapy holds great promise.However, the underlying mechanisms remain unclear.We found that hNSCs significantly decrease the CD3+ and CD8+ T cells, reduce the gamma delta T cells and increase the regulatory T cells, along with reduced pro-inflammatory cytokines and increased anti-inflammatory cytokines after co-culture.

View Article: PubMed Central - PubMed

Affiliation: Zhejiang Provincial Key Laboratory of Aging and Neurological Disorder Research, First Affiliated Hospital, Wenzhou Medical University, Wenzhou 35000, China.

ABSTRACT
Transplantation of human neural stem/progenitor cells (hNSCs) as a regenerative cell replacement therapy holds great promise. However, the underlying mechanisms remain unclear. We, here, focused on the interaction between hNSCs and allogeneic peripheral blood mononuclear cells (PBMCs) in a co-culture model. We found that hNSCs significantly decrease the CD3+ and CD8+ T cells, reduce the gamma delta T cells and increase the regulatory T cells, along with reduced pro-inflammatory cytokines and increased anti-inflammatory cytokines after co-culture. We also found that PBMCs, in turn, significantly promote the proliferation and differentiation of hNSCs. Our data suggest that hNSCs cross-talk with immune cells.

Show MeSH
Related in: MedlinePlus