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Impact of metronidazole and amoxicillin combination on matrix metalloproteinases-1 and tissue inhibitors of matrix metalloproteinases balance in generalized aggressive periodontitis.

Cifcibasi E, Kantarci A, Badur S, Issever H, Cintan S - Eur J Dent (2015 Jan-Mar)

Bottom Line: Total MMP-1 levels were significantly decreased in both groups (P < 0.05) at 3 and 6 months.MMP-1 concentration levels showed a similar pattern to MMP-1 total levels decreasing significantly at 3 months (P < 0.05).TIMP-1 concentration levels increased in the test group throughout the study period, while the difference did not reach statistical significance (P > 0.05).

View Article: PubMed Central - PubMed

Affiliation: Department of Periodontology, Faculty of Dentistry, Istanbul University, Istanbul, Turkiye.

ABSTRACT

Objective: Generalized aggressive periodontitis (GAgP) is a complex periodontal disease affecting the entire dentition with a rapid destruction of the periodontium and resulting in loss of teeth. We hypothesized that better clinical healing of adjunctive use of amoxicillin plus metronidazole combination may be related to the effect of this combination therapy to restore imbalance between matrix metalloproteinases (MMP) and their tissue inhibitors (TIMP) which is associated with connective tissue and alveolar bone destruction in patients with GAgP.

Materials and methods: Twenty-eight subjects diagnosed with GAgP were recruited. Patients were randomly assigned to test or control groups. MMP-1/TIMP-1 ratio was compared between groups receiving scaling and root planning (SRP) alone (control) or in combination with amoxicillin plus metronidazole (test). Clinical periodontal variables were measured. Gingival crevicular fluid samples were obtained and analyzed for MMP-1 and TIMP-1. Measurements were taken at baseline and repeated at 3 and 6 months after therapy.

Results: Total MMP-1 levels were significantly decreased in both groups (P < 0.05) at 3 and 6 months. MMP-1 concentration levels showed a similar pattern to MMP-1 total levels decreasing significantly at 3 months (P < 0.05). TIMP-1 concentration levels increased in the test group throughout the study period, while the difference did not reach statistical significance (P > 0.05). TIMP-1/MMP-1 balance was restored in test group at 6 months significantly better than the control group (P < 0.05).

Conclusion: The results of this study suggest that metronidazole and amoxicillin combination as an adjunct to SRP results in better clinical healing through restoring TIMP-1/MMP-1 balance.

No MeSH data available.


Related in: MedlinePlus

Tissue inhibitors of matrix metalloproteinases (TIMP-1) and MMP-1 levels in gingival crevicular fluid. *P < 0.05 compared to baseline. Repeated measures of analysis of variance and generalized linear model were used to detect intragroup and intergroup differences
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Figure 2: Tissue inhibitors of matrix metalloproteinases (TIMP-1) and MMP-1 levels in gingival crevicular fluid. *P < 0.05 compared to baseline. Repeated measures of analysis of variance and generalized linear model were used to detect intragroup and intergroup differences

Mentions: Figure 2 shows the changes in concentration and total levels of MMP-1 and TIMP-1 in the test and control groups during the course of the study. Total TIMP-1 levels did not significantly change at 3 months or after 6 months in study groups (P > 0.05). A pattern similar to that of total TIMP-1 levels was observed for the TIMP-1 concentration in the control group. Compared to the control group, the increase in test group was more stable for the concentration of TIMP-1 while the difference was not statistically significant (P > 0.05). Total MMP-1 levels were significantly decreased in both groups (P < 0.05) at 3 and 6 months. When we evaluated the MMP-1 concentration levels, a similar pattern was observed; the decrease at 3 months was significant (P < 0.05).


Impact of metronidazole and amoxicillin combination on matrix metalloproteinases-1 and tissue inhibitors of matrix metalloproteinases balance in generalized aggressive periodontitis.

Cifcibasi E, Kantarci A, Badur S, Issever H, Cintan S - Eur J Dent (2015 Jan-Mar)

Tissue inhibitors of matrix metalloproteinases (TIMP-1) and MMP-1 levels in gingival crevicular fluid. *P < 0.05 compared to baseline. Repeated measures of analysis of variance and generalized linear model were used to detect intragroup and intergroup differences
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4319300&req=5

Figure 2: Tissue inhibitors of matrix metalloproteinases (TIMP-1) and MMP-1 levels in gingival crevicular fluid. *P < 0.05 compared to baseline. Repeated measures of analysis of variance and generalized linear model were used to detect intragroup and intergroup differences
Mentions: Figure 2 shows the changes in concentration and total levels of MMP-1 and TIMP-1 in the test and control groups during the course of the study. Total TIMP-1 levels did not significantly change at 3 months or after 6 months in study groups (P > 0.05). A pattern similar to that of total TIMP-1 levels was observed for the TIMP-1 concentration in the control group. Compared to the control group, the increase in test group was more stable for the concentration of TIMP-1 while the difference was not statistically significant (P > 0.05). Total MMP-1 levels were significantly decreased in both groups (P < 0.05) at 3 and 6 months. When we evaluated the MMP-1 concentration levels, a similar pattern was observed; the decrease at 3 months was significant (P < 0.05).

Bottom Line: Total MMP-1 levels were significantly decreased in both groups (P < 0.05) at 3 and 6 months.MMP-1 concentration levels showed a similar pattern to MMP-1 total levels decreasing significantly at 3 months (P < 0.05).TIMP-1 concentration levels increased in the test group throughout the study period, while the difference did not reach statistical significance (P > 0.05).

View Article: PubMed Central - PubMed

Affiliation: Department of Periodontology, Faculty of Dentistry, Istanbul University, Istanbul, Turkiye.

ABSTRACT

Objective: Generalized aggressive periodontitis (GAgP) is a complex periodontal disease affecting the entire dentition with a rapid destruction of the periodontium and resulting in loss of teeth. We hypothesized that better clinical healing of adjunctive use of amoxicillin plus metronidazole combination may be related to the effect of this combination therapy to restore imbalance between matrix metalloproteinases (MMP) and their tissue inhibitors (TIMP) which is associated with connective tissue and alveolar bone destruction in patients with GAgP.

Materials and methods: Twenty-eight subjects diagnosed with GAgP were recruited. Patients were randomly assigned to test or control groups. MMP-1/TIMP-1 ratio was compared between groups receiving scaling and root planning (SRP) alone (control) or in combination with amoxicillin plus metronidazole (test). Clinical periodontal variables were measured. Gingival crevicular fluid samples were obtained and analyzed for MMP-1 and TIMP-1. Measurements were taken at baseline and repeated at 3 and 6 months after therapy.

Results: Total MMP-1 levels were significantly decreased in both groups (P < 0.05) at 3 and 6 months. MMP-1 concentration levels showed a similar pattern to MMP-1 total levels decreasing significantly at 3 months (P < 0.05). TIMP-1 concentration levels increased in the test group throughout the study period, while the difference did not reach statistical significance (P > 0.05). TIMP-1/MMP-1 balance was restored in test group at 6 months significantly better than the control group (P < 0.05).

Conclusion: The results of this study suggest that metronidazole and amoxicillin combination as an adjunct to SRP results in better clinical healing through restoring TIMP-1/MMP-1 balance.

No MeSH data available.


Related in: MedlinePlus