Semi-automated selection of cryo-EM particles in RELION-1.3.
Bottom Line: Here, a semi-automated particle selection procedure is presented that has been implemented within the open-source software RELION.With only limited user-interaction, the proposed procedure yields results that are comparable to manual particle selection.Together with an improved graphical user interface, these developments further contribute to turning RELION from a stand-alone refinement program into a convenient image processing pipeline for the entire single-particle approach.
Affiliation: MRC Laboratory of Molecular Biology, Cambridge Biomedical Campus, Francis Crick Avenue, Cambridge CB2 0QH, UK. Electronic address: firstname.lastname@example.org.Show MeSH
Mentions: The resulting average Z-scores may then be used for displaying the particles in a sorted manner. Particles with a high average Z-score are often high-contrast false positives such as ice, protein aggregates, carbon edges or pieces of junk, e.g. Fig. 3C. Therefore, visual inspection of the particles on the high-end of the sorted average Z-scores may be a more efficient way of getting rid of bad particles than inspecting the entire data set.
Affiliation: MRC Laboratory of Molecular Biology, Cambridge Biomedical Campus, Francis Crick Avenue, Cambridge CB2 0QH, UK. Electronic address: email@example.com.