Semi-automated selection of cryo-EM particles in RELION-1.3.
Bottom Line: Here, a semi-automated particle selection procedure is presented that has been implemented within the open-source software RELION.With only limited user-interaction, the proposed procedure yields results that are comparable to manual particle selection.Together with an improved graphical user interface, these developments further contribute to turning RELION from a stand-alone refinement program into a convenient image processing pipeline for the entire single-particle approach.
Affiliation: MRC Laboratory of Molecular Biology, Cambridge Biomedical Campus, Francis Crick Avenue, Cambridge CB2 0QH, UK. Electronic address: firstname.lastname@example.org.Show MeSH
Mentions: The template-based particle picking algorithm proposed employs an additive model with white Gaussian noise in real-space. A micrograph that contains N individual particles i at coordinates in the micrograph is described as follows (also see Fig. 1A):(1)X(r→)=μ(r→)+σ(r→)∗N(r→)+∑i=1NAkiϕi(r→-t→i),where:•
Affiliation: MRC Laboratory of Molecular Biology, Cambridge Biomedical Campus, Francis Crick Avenue, Cambridge CB2 0QH, UK. Electronic address: email@example.com.