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Cell type-specific effects of adenosine on cortical neurons.

van Aerde KI, Qi G, Feldmeyer D - Cereb. Cortex (2013)

Bottom Line: Although the effect of adenosine on subcortical areas has been previously described, the effects on cortical neurons have not been addressed systematically to date.We found that adenosine, via the A1 receptor, exerts differential effects depending on neuronal cell type and laminar location.These studies of the action of adenosine at the postsynaptic level may contribute to the understanding of the changes in cortical circuit functioning that take place between sleep and awakening.

View Article: PubMed Central - PubMed

Affiliation: Forschungszentrum Jülich, Institute of Neuroscience and Medicine, INM-2, D-52425 Jülich, Germany Current address: Netherlands Institute for Neuroscience, Royal Netherlands Academy of Arts and Science, 1105 BA Amsterdam, The Netherlands.

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Layer 6 pyramidal neurons are sensitive for adenosine. (A) Example traces of the RMP during bath application of 100 μM adenosine (start at arrow). Average response is shown in black (bottom, n = 13). (B) Morphological reconstruction of soma and dendrites from L6 pyramidal cells with apical dendrites reaching layer 1 (“tall”), or staying in layer 5–6 (“small” and “short and broad”). Small L6 neurons possessed more confined dendrites compared with “short and broad” L6 neurons. Note, however, the large morphological variability of L6 neurons. (C) Adenosine-induced hyperpolarization of the RMP for the different L6 morphological subtypes (tall, n = 4; small, n = 3, short and broad, n = 3; P = 0.46).
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BHT274F5: Layer 6 pyramidal neurons are sensitive for adenosine. (A) Example traces of the RMP during bath application of 100 μM adenosine (start at arrow). Average response is shown in black (bottom, n = 13). (B) Morphological reconstruction of soma and dendrites from L6 pyramidal cells with apical dendrites reaching layer 1 (“tall”), or staying in layer 5–6 (“small” and “short and broad”). Small L6 neurons possessed more confined dendrites compared with “short and broad” L6 neurons. Note, however, the large morphological variability of L6 neurons. (C) Adenosine-induced hyperpolarization of the RMP for the different L6 morphological subtypes (tall, n = 4; small, n = 3, short and broad, n = 3; P = 0.46).

Mentions: All pyramidal neurons in layer 6 showed a hyperpolarizing response to adenosine application and a trend toward a decrease in cell excitability (Fig. 5A and Table 1).Figure 5.


Cell type-specific effects of adenosine on cortical neurons.

van Aerde KI, Qi G, Feldmeyer D - Cereb. Cortex (2013)

Layer 6 pyramidal neurons are sensitive for adenosine. (A) Example traces of the RMP during bath application of 100 μM adenosine (start at arrow). Average response is shown in black (bottom, n = 13). (B) Morphological reconstruction of soma and dendrites from L6 pyramidal cells with apical dendrites reaching layer 1 (“tall”), or staying in layer 5–6 (“small” and “short and broad”). Small L6 neurons possessed more confined dendrites compared with “short and broad” L6 neurons. Note, however, the large morphological variability of L6 neurons. (C) Adenosine-induced hyperpolarization of the RMP for the different L6 morphological subtypes (tall, n = 4; small, n = 3, short and broad, n = 3; P = 0.46).
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4318536&req=5

BHT274F5: Layer 6 pyramidal neurons are sensitive for adenosine. (A) Example traces of the RMP during bath application of 100 μM adenosine (start at arrow). Average response is shown in black (bottom, n = 13). (B) Morphological reconstruction of soma and dendrites from L6 pyramidal cells with apical dendrites reaching layer 1 (“tall”), or staying in layer 5–6 (“small” and “short and broad”). Small L6 neurons possessed more confined dendrites compared with “short and broad” L6 neurons. Note, however, the large morphological variability of L6 neurons. (C) Adenosine-induced hyperpolarization of the RMP for the different L6 morphological subtypes (tall, n = 4; small, n = 3, short and broad, n = 3; P = 0.46).
Mentions: All pyramidal neurons in layer 6 showed a hyperpolarizing response to adenosine application and a trend toward a decrease in cell excitability (Fig. 5A and Table 1).Figure 5.

Bottom Line: Although the effect of adenosine on subcortical areas has been previously described, the effects on cortical neurons have not been addressed systematically to date.We found that adenosine, via the A1 receptor, exerts differential effects depending on neuronal cell type and laminar location.These studies of the action of adenosine at the postsynaptic level may contribute to the understanding of the changes in cortical circuit functioning that take place between sleep and awakening.

View Article: PubMed Central - PubMed

Affiliation: Forschungszentrum Jülich, Institute of Neuroscience and Medicine, INM-2, D-52425 Jülich, Germany Current address: Netherlands Institute for Neuroscience, Royal Netherlands Academy of Arts and Science, 1105 BA Amsterdam, The Netherlands.

Show MeSH
Related in: MedlinePlus