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The early fetal development of human neocortical GABAergic interneurons.

Al-Jaberi N, Lindsay S, Sarma S, Bayatti N, Clowry GJ - Cereb. Cortex (2013)

Bottom Line: CALB2-positive cells increased steadily in the SVZ/VZ from 10 PCW but were not double-labeled with Ki-67.Expression of GABAergic genes was generally higher in the dorsal pallium than in the ganglionic eminences, with lower expression in the intervening ventral pallium.It is widely accepted that the cortical proliferative zones may generate CALB2-positive interneurons from mid-gestation; we now show that the anterior neocortical proliferative layers especially may be a rich source of interneurons in the early neocortex.

View Article: PubMed Central - PubMed

Affiliation: Institute of Neuroscience Institute of Genetic Medicine, Newcastle University, Newcastle upon Tyne NE1 3BZ, UK.

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Laminar expression of GABAergic immunoreactivity at 9 PCW. ASCL1, DLX2, and GAD2 show similar patterns of immunoreactivity at 9 PCW, with a mosaic of cells exhibiting either strong, moderate, and weak/no expression throughout the layers of the cortical wall. They differed in that ASCL1 expression was generally more prominent in the VZ, whereas DLX2 was stronger in the SVZ. GAD2 immunoreactivity was observed in proliferative layers as well as in postmitotic cells. In the cortical plate (CP), most GABAergic markers showed more expression in cells closer to the outer boundary with the marginal zone. The exception was CALB2 immunoreactivity, which was most prominent in cells at the interface between the CP and the early subplate (SP/IZ). CALB2-positive fibrers were also seen extending into the intermediate zone (IZ), but no CALB2-positive cells were seen in the SVZ/VZ at this stage. Scale bar = 200 μm.
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BHT254F7: Laminar expression of GABAergic immunoreactivity at 9 PCW. ASCL1, DLX2, and GAD2 show similar patterns of immunoreactivity at 9 PCW, with a mosaic of cells exhibiting either strong, moderate, and weak/no expression throughout the layers of the cortical wall. They differed in that ASCL1 expression was generally more prominent in the VZ, whereas DLX2 was stronger in the SVZ. GAD2 immunoreactivity was observed in proliferative layers as well as in postmitotic cells. In the cortical plate (CP), most GABAergic markers showed more expression in cells closer to the outer boundary with the marginal zone. The exception was CALB2 immunoreactivity, which was most prominent in cells at the interface between the CP and the early subplate (SP/IZ). CALB2-positive fibrers were also seen extending into the intermediate zone (IZ), but no CALB2-positive cells were seen in the SVZ/VZ at this stage. Scale bar = 200 μm.

Mentions: At 6.5–7.5 PCW, we confirmed the presence of GAD2 and CALB2 expressing neurons in the preplate as previously described (Meyer et al. 2000; Rakic and Zecevic 2003). In addition, DLX2 and ASCL1 immunoreactivity was observed. For all markers, positive cells were seen in VZ, sometimes in radially arranged clusters (Fig. 6). ASCL1 immunoreactivity was more prevalent in the VZ, whereas the others showed stronger expression in the preplate. This fits with the idea that ASCL1 is expressed earlier in the development of interneurons (see Introduction section) and strongly suggests that GABAergic cells are born in the cortical wall at this stage of development. ASCL1, DLX2, and GAD2 showed similar patterns of immunoreactivity between 8 and 12 PCW, with a mosaic of cells exhibiting either strong, moderate, and weak/no expression throughout the layers of the cortical wall (Fig. 7). They differ in that ASCL1 expression is generally more prominent in the VZ, whereas DLX2 was stronger in the SVZ. Despite being a marker for synaptic terminals in adult tissue (Erlander et al. 1991; Esclapez et al. 1994), GAD2 immunoreactivity was observed in proliferative layers (Fig. 6) as has been previously described (Petanjek et al. 2009). GAD1 TISH staining was also most prominent in the SVZ and CP at 9 PCW (Fig. 3C). In the CP, most GABAergic markers showed more expression in cells closer to the outer boundary with the marginal zone. The exception was CALB2, immunoreactivity which was most prominent in cells at the interface between the CP and the early SP. This confirms the previous suggestion (Meyer et al. 2000) that these CALB2 cells are pioneer cells sending axons toward the thalamus rather than GABAergic cells. CALB2-positive axons can be observed in the early SP, intermediate zone, and into the internal capsule and CALB2 positive GABAergic neurons may exist at other locations in the CP and early SP.Figure 6.


The early fetal development of human neocortical GABAergic interneurons.

Al-Jaberi N, Lindsay S, Sarma S, Bayatti N, Clowry GJ - Cereb. Cortex (2013)

Laminar expression of GABAergic immunoreactivity at 9 PCW. ASCL1, DLX2, and GAD2 show similar patterns of immunoreactivity at 9 PCW, with a mosaic of cells exhibiting either strong, moderate, and weak/no expression throughout the layers of the cortical wall. They differed in that ASCL1 expression was generally more prominent in the VZ, whereas DLX2 was stronger in the SVZ. GAD2 immunoreactivity was observed in proliferative layers as well as in postmitotic cells. In the cortical plate (CP), most GABAergic markers showed more expression in cells closer to the outer boundary with the marginal zone. The exception was CALB2 immunoreactivity, which was most prominent in cells at the interface between the CP and the early subplate (SP/IZ). CALB2-positive fibrers were also seen extending into the intermediate zone (IZ), but no CALB2-positive cells were seen in the SVZ/VZ at this stage. Scale bar = 200 μm.
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Related In: Results  -  Collection

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BHT254F7: Laminar expression of GABAergic immunoreactivity at 9 PCW. ASCL1, DLX2, and GAD2 show similar patterns of immunoreactivity at 9 PCW, with a mosaic of cells exhibiting either strong, moderate, and weak/no expression throughout the layers of the cortical wall. They differed in that ASCL1 expression was generally more prominent in the VZ, whereas DLX2 was stronger in the SVZ. GAD2 immunoreactivity was observed in proliferative layers as well as in postmitotic cells. In the cortical plate (CP), most GABAergic markers showed more expression in cells closer to the outer boundary with the marginal zone. The exception was CALB2 immunoreactivity, which was most prominent in cells at the interface between the CP and the early subplate (SP/IZ). CALB2-positive fibrers were also seen extending into the intermediate zone (IZ), but no CALB2-positive cells were seen in the SVZ/VZ at this stage. Scale bar = 200 μm.
Mentions: At 6.5–7.5 PCW, we confirmed the presence of GAD2 and CALB2 expressing neurons in the preplate as previously described (Meyer et al. 2000; Rakic and Zecevic 2003). In addition, DLX2 and ASCL1 immunoreactivity was observed. For all markers, positive cells were seen in VZ, sometimes in radially arranged clusters (Fig. 6). ASCL1 immunoreactivity was more prevalent in the VZ, whereas the others showed stronger expression in the preplate. This fits with the idea that ASCL1 is expressed earlier in the development of interneurons (see Introduction section) and strongly suggests that GABAergic cells are born in the cortical wall at this stage of development. ASCL1, DLX2, and GAD2 showed similar patterns of immunoreactivity between 8 and 12 PCW, with a mosaic of cells exhibiting either strong, moderate, and weak/no expression throughout the layers of the cortical wall (Fig. 7). They differ in that ASCL1 expression is generally more prominent in the VZ, whereas DLX2 was stronger in the SVZ. Despite being a marker for synaptic terminals in adult tissue (Erlander et al. 1991; Esclapez et al. 1994), GAD2 immunoreactivity was observed in proliferative layers (Fig. 6) as has been previously described (Petanjek et al. 2009). GAD1 TISH staining was also most prominent in the SVZ and CP at 9 PCW (Fig. 3C). In the CP, most GABAergic markers showed more expression in cells closer to the outer boundary with the marginal zone. The exception was CALB2, immunoreactivity which was most prominent in cells at the interface between the CP and the early SP. This confirms the previous suggestion (Meyer et al. 2000) that these CALB2 cells are pioneer cells sending axons toward the thalamus rather than GABAergic cells. CALB2-positive axons can be observed in the early SP, intermediate zone, and into the internal capsule and CALB2 positive GABAergic neurons may exist at other locations in the CP and early SP.Figure 6.

Bottom Line: CALB2-positive cells increased steadily in the SVZ/VZ from 10 PCW but were not double-labeled with Ki-67.Expression of GABAergic genes was generally higher in the dorsal pallium than in the ganglionic eminences, with lower expression in the intervening ventral pallium.It is widely accepted that the cortical proliferative zones may generate CALB2-positive interneurons from mid-gestation; we now show that the anterior neocortical proliferative layers especially may be a rich source of interneurons in the early neocortex.

View Article: PubMed Central - PubMed

Affiliation: Institute of Neuroscience Institute of Genetic Medicine, Newcastle University, Newcastle upon Tyne NE1 3BZ, UK.

Show MeSH
Related in: MedlinePlus