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The early fetal development of human neocortical GABAergic interneurons.

Al-Jaberi N, Lindsay S, Sarma S, Bayatti N, Clowry GJ - Cereb. Cortex (2013)

Bottom Line: CALB2-positive cells increased steadily in the SVZ/VZ from 10 PCW but were not double-labeled with Ki-67.Expression of GABAergic genes was generally higher in the dorsal pallium than in the ganglionic eminences, with lower expression in the intervening ventral pallium.It is widely accepted that the cortical proliferative zones may generate CALB2-positive interneurons from mid-gestation; we now show that the anterior neocortical proliferative layers especially may be a rich source of interneurons in the early neocortex.

View Article: PubMed Central - PubMed

Affiliation: Institute of Neuroscience Institute of Genetic Medicine, Newcastle University, Newcastle upon Tyne NE1 3BZ, UK.

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Expression of GABAergic genes in horizontal sections at 8 PCW. Staining patterns for ISH for DLX5 (A), and IHC for CALB2 (B) in horizontal sections at 8 PCWs serve to further illustrate that there were 2 centers of strongest expression, in the ganglionic eminence, and in the dorsal pallium, particularly at more anterior (Ant) locations, with no evidence of a gradient of expression between the sub pallium and dorsal pallium that would suggest a migratory stream of cells moving between the 2. Indeed, there appeared to be distinct break in DLX5 expression between the postmitotic layers of the ganglionic eminence (GE) and cortical plate of the ventral pallium (arrow). However, at higher magnification, a few CALB2-positive neurons appeared to be crossing this zone (C, small arrows). Scale bar = 100 μm in A,B; 300 μm in C. The orientation of all the section images are the same, hence Ant is the same in B indicated in A (C is image of section in B at higher magnification).
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BHT254F5: Expression of GABAergic genes in horizontal sections at 8 PCW. Staining patterns for ISH for DLX5 (A), and IHC for CALB2 (B) in horizontal sections at 8 PCWs serve to further illustrate that there were 2 centers of strongest expression, in the ganglionic eminence, and in the dorsal pallium, particularly at more anterior (Ant) locations, with no evidence of a gradient of expression between the sub pallium and dorsal pallium that would suggest a migratory stream of cells moving between the 2. Indeed, there appeared to be distinct break in DLX5 expression between the postmitotic layers of the ganglionic eminence (GE) and cortical plate of the ventral pallium (arrow). However, at higher magnification, a few CALB2-positive neurons appeared to be crossing this zone (C, small arrows). Scale bar = 100 μm in A,B; 300 μm in C. The orientation of all the section images are the same, hence Ant is the same in B indicated in A (C is image of section in B at higher magnification).

Mentions: Whole sagittal sections of fetal brain were also successfully immunostained for 4 markers of GABAergic interneurons, DLX2, ASCL1, GAD2, and CALB2 between 8 and 12 PCW weeks. At all stages, a similar expression pattern was observed for DLX2, ASCL1, and GAD2, with moderate expression in the GE, but stronger expression in the CP (Fig. 4). There was also some expression in the VZ/SVZ when compared with areas of completely negative staining in the diencephalon. CALB2 showed a different pattern of staining. CALB2-positive neurons were present in the postmitotic layers of the lateral (Fig. 5B,C) and caudal (not shown) GE, but were absent from the MGE (Fig. 4D). In the cortex, CALB2-positive neuronal cell bodies were prominent in the cortical plate (CP) at 8–9 PCW, with fewer also present in the marginal zone and subplate, but with many immunoreactive fibers present in the subplate and intermediate zone (Figs 4D and 5B). No immunostaining was present in the proliferative zones at these earlier stages.Figure 4.


The early fetal development of human neocortical GABAergic interneurons.

Al-Jaberi N, Lindsay S, Sarma S, Bayatti N, Clowry GJ - Cereb. Cortex (2013)

Expression of GABAergic genes in horizontal sections at 8 PCW. Staining patterns for ISH for DLX5 (A), and IHC for CALB2 (B) in horizontal sections at 8 PCWs serve to further illustrate that there were 2 centers of strongest expression, in the ganglionic eminence, and in the dorsal pallium, particularly at more anterior (Ant) locations, with no evidence of a gradient of expression between the sub pallium and dorsal pallium that would suggest a migratory stream of cells moving between the 2. Indeed, there appeared to be distinct break in DLX5 expression between the postmitotic layers of the ganglionic eminence (GE) and cortical plate of the ventral pallium (arrow). However, at higher magnification, a few CALB2-positive neurons appeared to be crossing this zone (C, small arrows). Scale bar = 100 μm in A,B; 300 μm in C. The orientation of all the section images are the same, hence Ant is the same in B indicated in A (C is image of section in B at higher magnification).
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4318531&req=5

BHT254F5: Expression of GABAergic genes in horizontal sections at 8 PCW. Staining patterns for ISH for DLX5 (A), and IHC for CALB2 (B) in horizontal sections at 8 PCWs serve to further illustrate that there were 2 centers of strongest expression, in the ganglionic eminence, and in the dorsal pallium, particularly at more anterior (Ant) locations, with no evidence of a gradient of expression between the sub pallium and dorsal pallium that would suggest a migratory stream of cells moving between the 2. Indeed, there appeared to be distinct break in DLX5 expression between the postmitotic layers of the ganglionic eminence (GE) and cortical plate of the ventral pallium (arrow). However, at higher magnification, a few CALB2-positive neurons appeared to be crossing this zone (C, small arrows). Scale bar = 100 μm in A,B; 300 μm in C. The orientation of all the section images are the same, hence Ant is the same in B indicated in A (C is image of section in B at higher magnification).
Mentions: Whole sagittal sections of fetal brain were also successfully immunostained for 4 markers of GABAergic interneurons, DLX2, ASCL1, GAD2, and CALB2 between 8 and 12 PCW weeks. At all stages, a similar expression pattern was observed for DLX2, ASCL1, and GAD2, with moderate expression in the GE, but stronger expression in the CP (Fig. 4). There was also some expression in the VZ/SVZ when compared with areas of completely negative staining in the diencephalon. CALB2 showed a different pattern of staining. CALB2-positive neurons were present in the postmitotic layers of the lateral (Fig. 5B,C) and caudal (not shown) GE, but were absent from the MGE (Fig. 4D). In the cortex, CALB2-positive neuronal cell bodies were prominent in the cortical plate (CP) at 8–9 PCW, with fewer also present in the marginal zone and subplate, but with many immunoreactive fibers present in the subplate and intermediate zone (Figs 4D and 5B). No immunostaining was present in the proliferative zones at these earlier stages.Figure 4.

Bottom Line: CALB2-positive cells increased steadily in the SVZ/VZ from 10 PCW but were not double-labeled with Ki-67.Expression of GABAergic genes was generally higher in the dorsal pallium than in the ganglionic eminences, with lower expression in the intervening ventral pallium.It is widely accepted that the cortical proliferative zones may generate CALB2-positive interneurons from mid-gestation; we now show that the anterior neocortical proliferative layers especially may be a rich source of interneurons in the early neocortex.

View Article: PubMed Central - PubMed

Affiliation: Institute of Neuroscience Institute of Genetic Medicine, Newcastle University, Newcastle upon Tyne NE1 3BZ, UK.

Show MeSH
Related in: MedlinePlus