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The early fetal development of human neocortical GABAergic interneurons.

Al-Jaberi N, Lindsay S, Sarma S, Bayatti N, Clowry GJ - Cereb. Cortex (2013)

Bottom Line: CALB2-positive cells increased steadily in the SVZ/VZ from 10 PCW but were not double-labeled with Ki-67.Expression of GABAergic genes was generally higher in the dorsal pallium than in the ganglionic eminences, with lower expression in the intervening ventral pallium.It is widely accepted that the cortical proliferative zones may generate CALB2-positive interneurons from mid-gestation; we now show that the anterior neocortical proliferative layers especially may be a rich source of interneurons in the early neocortex.

View Article: PubMed Central - PubMed

Affiliation: Institute of Neuroscience Institute of Genetic Medicine, Newcastle University, Newcastle upon Tyne NE1 3BZ, UK.

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Temporal changes in GABAergic gene expression by qPCR between 8 and 12 PCW. The expression of the genes of interest, relative to the average expression of 3 reference genes, from RNA samples taken from the anterior and posterior poles of the human neocortex between 8 and 12 PCW, is shown for qPCR data collected in the present study (n = 8 fetuses). Statistically significant linear correlations of expression over time (P < 0.05) are marked with a line. It can be seen that nearly all genes showed increased expression, both anteriorly and posteriorly, with age, although for some genes, notably DLX5 and GABRB3, expression increased more quickly anteriorly, compared with posteriorly. FGFR3 expression is included as an example of a posteriorly expressed gene.
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BHT254F2: Temporal changes in GABAergic gene expression by qPCR between 8 and 12 PCW. The expression of the genes of interest, relative to the average expression of 3 reference genes, from RNA samples taken from the anterior and posterior poles of the human neocortex between 8 and 12 PCW, is shown for qPCR data collected in the present study (n = 8 fetuses). Statistically significant linear correlations of expression over time (P < 0.05) are marked with a line. It can be seen that nearly all genes showed increased expression, both anteriorly and posteriorly, with age, although for some genes, notably DLX5 and GABRB3, expression increased more quickly anteriorly, compared with posteriorly. FGFR3 expression is included as an example of a posteriorly expressed gene.

Mentions: Consistent patterns of expression over time were also observed (Fig. 2). Nearly all genes showed increasing expression with age. In most cases, anterior and posterior expression levels increased in parallel, although anterior was consistently higher than posterior. For some genes, notably DLX1, DLX5, and GABRB3, expression at the anterior pole increased at a faster rate than at the posterior pole. ISH studies confirmed the expression of 4 of these genes, DLX1, 2, and 5 and GAD1, and all showed gradients of expression from anterior to posterior poles at 8 and 9 PCW (Fig. 3, DLX2 not shown). Although expression of these genes was detected by ISH in the GE, as would be expected from animal studies, expression was higher in the neocortex, particularly at the anterior pole.Figure 2.


The early fetal development of human neocortical GABAergic interneurons.

Al-Jaberi N, Lindsay S, Sarma S, Bayatti N, Clowry GJ - Cereb. Cortex (2013)

Temporal changes in GABAergic gene expression by qPCR between 8 and 12 PCW. The expression of the genes of interest, relative to the average expression of 3 reference genes, from RNA samples taken from the anterior and posterior poles of the human neocortex between 8 and 12 PCW, is shown for qPCR data collected in the present study (n = 8 fetuses). Statistically significant linear correlations of expression over time (P < 0.05) are marked with a line. It can be seen that nearly all genes showed increased expression, both anteriorly and posteriorly, with age, although for some genes, notably DLX5 and GABRB3, expression increased more quickly anteriorly, compared with posteriorly. FGFR3 expression is included as an example of a posteriorly expressed gene.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4318531&req=5

BHT254F2: Temporal changes in GABAergic gene expression by qPCR between 8 and 12 PCW. The expression of the genes of interest, relative to the average expression of 3 reference genes, from RNA samples taken from the anterior and posterior poles of the human neocortex between 8 and 12 PCW, is shown for qPCR data collected in the present study (n = 8 fetuses). Statistically significant linear correlations of expression over time (P < 0.05) are marked with a line. It can be seen that nearly all genes showed increased expression, both anteriorly and posteriorly, with age, although for some genes, notably DLX5 and GABRB3, expression increased more quickly anteriorly, compared with posteriorly. FGFR3 expression is included as an example of a posteriorly expressed gene.
Mentions: Consistent patterns of expression over time were also observed (Fig. 2). Nearly all genes showed increasing expression with age. In most cases, anterior and posterior expression levels increased in parallel, although anterior was consistently higher than posterior. For some genes, notably DLX1, DLX5, and GABRB3, expression at the anterior pole increased at a faster rate than at the posterior pole. ISH studies confirmed the expression of 4 of these genes, DLX1, 2, and 5 and GAD1, and all showed gradients of expression from anterior to posterior poles at 8 and 9 PCW (Fig. 3, DLX2 not shown). Although expression of these genes was detected by ISH in the GE, as would be expected from animal studies, expression was higher in the neocortex, particularly at the anterior pole.Figure 2.

Bottom Line: CALB2-positive cells increased steadily in the SVZ/VZ from 10 PCW but were not double-labeled with Ki-67.Expression of GABAergic genes was generally higher in the dorsal pallium than in the ganglionic eminences, with lower expression in the intervening ventral pallium.It is widely accepted that the cortical proliferative zones may generate CALB2-positive interneurons from mid-gestation; we now show that the anterior neocortical proliferative layers especially may be a rich source of interneurons in the early neocortex.

View Article: PubMed Central - PubMed

Affiliation: Institute of Neuroscience Institute of Genetic Medicine, Newcastle University, Newcastle upon Tyne NE1 3BZ, UK.

Show MeSH
Related in: MedlinePlus