The early fetal development of human neocortical GABAergic interneurons.
Bottom Line: CALB2-positive cells increased steadily in the SVZ/VZ from 10 PCW but were not double-labeled with Ki-67.Expression of GABAergic genes was generally higher in the dorsal pallium than in the ganglionic eminences, with lower expression in the intervening ventral pallium.It is widely accepted that the cortical proliferative zones may generate CALB2-positive interneurons from mid-gestation; we now show that the anterior neocortical proliferative layers especially may be a rich source of interneurons in the early neocortex.
Affiliation: Institute of Neuroscience Institute of Genetic Medicine, Newcastle University, Newcastle upon Tyne NE1 3BZ, UK.Show MeSH
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Mentions: Consistent patterns of expression over time were also observed (Fig. 2). Nearly all genes showed increasing expression with age. In most cases, anterior and posterior expression levels increased in parallel, although anterior was consistently higher than posterior. For some genes, notably DLX1, DLX5, and GABRB3, expression at the anterior pole increased at a faster rate than at the posterior pole. ISH studies confirmed the expression of 4 of these genes, DLX1, 2, and 5 and GAD1, and all showed gradients of expression from anterior to posterior poles at 8 and 9 PCW (Fig. 3, DLX2 not shown). Although expression of these genes was detected by ISH in the GE, as would be expected from animal studies, expression was higher in the neocortex, particularly at the anterior pole.Figure 2.
Affiliation: Institute of Neuroscience Institute of Genetic Medicine, Newcastle University, Newcastle upon Tyne NE1 3BZ, UK.