The early fetal development of human neocortical GABAergic interneurons.
Bottom Line: CALB2-positive cells increased steadily in the SVZ/VZ from 10 PCW but were not double-labeled with Ki-67.Expression of GABAergic genes was generally higher in the dorsal pallium than in the ganglionic eminences, with lower expression in the intervening ventral pallium.It is widely accepted that the cortical proliferative zones may generate CALB2-positive interneurons from mid-gestation; we now show that the anterior neocortical proliferative layers especially may be a rich source of interneurons in the early neocortex.
Affiliation: Institute of Neuroscience Institute of Genetic Medicine, Newcastle University, Newcastle upon Tyne NE1 3BZ, UK.Show MeSH
Related in: MedlinePlus
Mentions: A qPCR study was carried out to validate and extend the results obtained from our previous microarray study (Ip et al. 2010). In addition, we accessed publically available RNA seq data (http://brainspan.org/rnaseq/search/index.html) at the relevant developmental time points, and expressed all 3 datasets in a way that makes them comparable (Fig. 1). All 3 approaches gave broadly similar patterns of gene expression with nearly all GABAergic genes selected for study showing higher expression at the anterior compared with the posterior pole. However, this only reached statistical significance for all markers in the qPCR study. This validates the approach of collecting global gene expression data by microarray or whole RNA sequencing as a way of discovering potentially interesting patterns of gene expression. Nevertheless, confirmation by qPCR and other methods are still required.Figure 1.
Affiliation: Institute of Neuroscience Institute of Genetic Medicine, Newcastle University, Newcastle upon Tyne NE1 3BZ, UK.