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New Chemometrics Mode Based on Adjacent Data Points' Differences for the Simultaneous Determination of Clopidogrel, Atorvastatin, and Aspirin in their Combined Ternary Drug Formulation.

Nejem RM, Issa MM, Shanab AA, Stefan-Van Staden RI, Aboul-Enein HY - Sci Pharm (2014)

Bottom Line: The method has been successfully applied to the simultaneous determination of ternary mixtures of aspirin, clopidogrel bisulphate, and atorvastatin calcium.The analytical characteristics of the method were calculated.The results showed that the new method is simple, rapid, accurate, and precise.

View Article: PubMed Central - PubMed

Affiliation: Analytical Chemistry, Department of Chemistry, Alaqsa University, P.O.Box 4051, Gaza, Palestine.

ABSTRACT
A new method is proposed for the analysis of a ternary mixture composed of clopidogrel, atorvastatin, and aspirin without prior separation steps. The method combines the advantages of the mean centering of ratio spectra and derivative spectrophotometric methods. It is based on using the difference between adjacent data points in the absorbance spectra. The principal advantage of this method is the use of absorbance data, and not derivative data; hence the signal-to-noise ratio is not diminished. The mathematical explanation of the procedure is illustrated. Beer's law was valid in the concentration range 0.3-35 μg.mL(-1) for CLOP, 0.5-30 μg.mL(-1) for ATOR, and 1-40 μg.mL(-1) for ASP. Mean recoveries were obtained as 100.2, 100.1, and 100.2% for CLOP, ATOR, and ASP, respectively, in the prepared synthetic mixtures. The method has been successfully applied to the simultaneous determination of ternary mixtures of aspirin, clopidogrel bisulphate, and atorvastatin calcium. The analytical characteristics of the method were calculated. The results showed that the new method is simple, rapid, accurate, and precise.

No MeSH data available.


Related in: MedlinePlus

The second ratio spectra of different concentrations (2.5, 5, 7.5, and 10 μg.mL-1) of ASP using the DBADP method
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Figure 3: The second ratio spectra of different concentrations (2.5, 5, 7.5, and 10 μg.mL-1) of ASP using the DBADP method

Mentions: The signals of the second ratio spectra at 260 (Fig. 2), 273 (Fig. 3), and 252 nm (Fig. 4) for CLOP, ASP, and ATOR, respectively, were measured and plotted against the corresponding concentrations of each drug to construct their calibration curves. The spectra of the different synthetic mixtures containing different ratios of CLOP, ASP, and ATOR were recorded and the previously explained procedure was performed to predict the concentration of each compound in the mixture.


New Chemometrics Mode Based on Adjacent Data Points' Differences for the Simultaneous Determination of Clopidogrel, Atorvastatin, and Aspirin in their Combined Ternary Drug Formulation.

Nejem RM, Issa MM, Shanab AA, Stefan-Van Staden RI, Aboul-Enein HY - Sci Pharm (2014)

The second ratio spectra of different concentrations (2.5, 5, 7.5, and 10 μg.mL-1) of ASP using the DBADP method
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4318198&req=5

Figure 3: The second ratio spectra of different concentrations (2.5, 5, 7.5, and 10 μg.mL-1) of ASP using the DBADP method
Mentions: The signals of the second ratio spectra at 260 (Fig. 2), 273 (Fig. 3), and 252 nm (Fig. 4) for CLOP, ASP, and ATOR, respectively, were measured and plotted against the corresponding concentrations of each drug to construct their calibration curves. The spectra of the different synthetic mixtures containing different ratios of CLOP, ASP, and ATOR were recorded and the previously explained procedure was performed to predict the concentration of each compound in the mixture.

Bottom Line: The method has been successfully applied to the simultaneous determination of ternary mixtures of aspirin, clopidogrel bisulphate, and atorvastatin calcium.The analytical characteristics of the method were calculated.The results showed that the new method is simple, rapid, accurate, and precise.

View Article: PubMed Central - PubMed

Affiliation: Analytical Chemistry, Department of Chemistry, Alaqsa University, P.O.Box 4051, Gaza, Palestine.

ABSTRACT
A new method is proposed for the analysis of a ternary mixture composed of clopidogrel, atorvastatin, and aspirin without prior separation steps. The method combines the advantages of the mean centering of ratio spectra and derivative spectrophotometric methods. It is based on using the difference between adjacent data points in the absorbance spectra. The principal advantage of this method is the use of absorbance data, and not derivative data; hence the signal-to-noise ratio is not diminished. The mathematical explanation of the procedure is illustrated. Beer's law was valid in the concentration range 0.3-35 μg.mL(-1) for CLOP, 0.5-30 μg.mL(-1) for ATOR, and 1-40 μg.mL(-1) for ASP. Mean recoveries were obtained as 100.2, 100.1, and 100.2% for CLOP, ATOR, and ASP, respectively, in the prepared synthetic mixtures. The method has been successfully applied to the simultaneous determination of ternary mixtures of aspirin, clopidogrel bisulphate, and atorvastatin calcium. The analytical characteristics of the method were calculated. The results showed that the new method is simple, rapid, accurate, and precise.

No MeSH data available.


Related in: MedlinePlus