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Evaluation of apoptosis in skin biopsies of patients of borderline leprosy and lepra type 1 reaction.

Patnaik N, Agarwal S, Sharma S, Sharma S, Pandhi D - Indian J Dermatol (2015 Jan-Feb)

Bottom Line: On quantitative estimation it was found that number of apoptotic bodies are higher in Group I in comparison to Group II (2.77 vs 1.99), which is statistically significant.Apoptosis was seen more in patients with T1R both qualitatively (statistically nonsignificant) and quantitatively (statistically significant).Clinical significance of this novel finding is that apoptosis can be used as one of the variables for diagnosis of T1R to increase detection rate.

View Article: PubMed Central - PubMed

Affiliation: Department of Pathology, University College of Medical Sciences and Guru Teg Bahadur Hospital, Shahdara, Delhi, India.

ABSTRACT

Background: The role of apoptosis is not clear in leprosy and lepra reactions.

Objectives: To evaluate frequency of apoptosis in skin lesions of borderline leprosy and Type 1 lepra reaction.

Methods: Sixty patients with borderline leprosy (30 with clinically diagnosed Type 1 reaction (T1R) (Group I) and 30 without clinical evidence of reaction (Group II)) were analyzed in this prospective study. Apoptosis was detected by two different methods for comparison, that is, histopathologic examination (HPE) and deoxyribonucleic acid (DNA) fragmentation and electrophoresis. Quantification of apoptotic bodies/10 high power fields (HPF) was also done.

Results: Out of 30 cases, apoptosis was detected in 29 cases in Group I and 24 cases in Group II by HPE (P = 0.103), whereas, with the use of DNA electrophoresis it was detected in 24 cases in Group I and 18 cases in Group II (P = 0.091). On quantitative estimation it was found that number of apoptotic bodies are higher in Group I in comparison to Group II (2.77 vs 1.99), which is statistically significant.

Conclusions: There was moderate agreement (κ = 0.47) between the two methods of apoptosis detection. Apoptosis was seen more in patients with T1R both qualitatively (statistically nonsignificant) and quantitatively (statistically significant). Clinical significance of this novel finding is that apoptosis can be used as one of the variables for diagnosis of T1R to increase detection rate.

No MeSH data available.


Related in: MedlinePlus

Agarose gel electrophoresis in Tris-acetate-ethylenediamine-tetra-acetic acid (TAE) buffer
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Figure 2: Agarose gel electrophoresis in Tris-acetate-ethylenediamine-tetra-acetic acid (TAE) buffer

Mentions: Macrophage apoptosis by HPE [Figure 1] was seen in 29/30 (96.7%) cases in Group I; whereas, 24/30 (80%) cases in Group II. While apoptosis by DNA electrophoresis [Figure 2] was seen in 24/30 (80%) cases in Group I and 18/30 (60%) cases in Group II.


Evaluation of apoptosis in skin biopsies of patients of borderline leprosy and lepra type 1 reaction.

Patnaik N, Agarwal S, Sharma S, Sharma S, Pandhi D - Indian J Dermatol (2015 Jan-Feb)

Agarose gel electrophoresis in Tris-acetate-ethylenediamine-tetra-acetic acid (TAE) buffer
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4318065&req=5

Figure 2: Agarose gel electrophoresis in Tris-acetate-ethylenediamine-tetra-acetic acid (TAE) buffer
Mentions: Macrophage apoptosis by HPE [Figure 1] was seen in 29/30 (96.7%) cases in Group I; whereas, 24/30 (80%) cases in Group II. While apoptosis by DNA electrophoresis [Figure 2] was seen in 24/30 (80%) cases in Group I and 18/30 (60%) cases in Group II.

Bottom Line: On quantitative estimation it was found that number of apoptotic bodies are higher in Group I in comparison to Group II (2.77 vs 1.99), which is statistically significant.Apoptosis was seen more in patients with T1R both qualitatively (statistically nonsignificant) and quantitatively (statistically significant).Clinical significance of this novel finding is that apoptosis can be used as one of the variables for diagnosis of T1R to increase detection rate.

View Article: PubMed Central - PubMed

Affiliation: Department of Pathology, University College of Medical Sciences and Guru Teg Bahadur Hospital, Shahdara, Delhi, India.

ABSTRACT

Background: The role of apoptosis is not clear in leprosy and lepra reactions.

Objectives: To evaluate frequency of apoptosis in skin lesions of borderline leprosy and Type 1 lepra reaction.

Methods: Sixty patients with borderline leprosy (30 with clinically diagnosed Type 1 reaction (T1R) (Group I) and 30 without clinical evidence of reaction (Group II)) were analyzed in this prospective study. Apoptosis was detected by two different methods for comparison, that is, histopathologic examination (HPE) and deoxyribonucleic acid (DNA) fragmentation and electrophoresis. Quantification of apoptotic bodies/10 high power fields (HPF) was also done.

Results: Out of 30 cases, apoptosis was detected in 29 cases in Group I and 24 cases in Group II by HPE (P = 0.103), whereas, with the use of DNA electrophoresis it was detected in 24 cases in Group I and 18 cases in Group II (P = 0.091). On quantitative estimation it was found that number of apoptotic bodies are higher in Group I in comparison to Group II (2.77 vs 1.99), which is statistically significant.

Conclusions: There was moderate agreement (κ = 0.47) between the two methods of apoptosis detection. Apoptosis was seen more in patients with T1R both qualitatively (statistically nonsignificant) and quantitatively (statistically significant). Clinical significance of this novel finding is that apoptosis can be used as one of the variables for diagnosis of T1R to increase detection rate.

No MeSH data available.


Related in: MedlinePlus