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Immunohistochemical expression of cathepsin L in atopic dermatitis and lichen planus.

Ibrahim ZA, El Ashmawy AA, Abd El-Naby NM, Ghoraba HM - Indian J Dermatol (2015 Jan-Feb)

Bottom Line: Elevated expression of cathepsin L has been found in many inflammatory and neoplastic diseases.Highly significant increase was found in cathepsin L expression in AD and LP patients compared to controls [P = 0.001].It could be a useful marker for assessing AD severity.

View Article: PubMed Central - PubMed

Affiliation: Department of Dermatology and Venereology, Faculty of Medicine, Tanta University, Tanta, Gharbia, Egypt.

ABSTRACT

Background: Cathepsin L is a member of papain superfamily. It seems to promote T-cell survival, selection maturation in the thymus and enhance the antigen presentation. Cathepsin L plays an important role in tumor necrosis factors (TNF-α) induced cell death. Also it degrades the tight junction between cornedesomses in the epidermis. Elevated expression of cathepsin L has been found in many inflammatory and neoplastic diseases.

Objective: The aim of this study was to determine immunohistochemical expression of cathepsin L in atopic dermatitis (AD) and lichen planus (LP) patients in order to evaluate its role in the pathogenesis of both diseases.

Materials and methods: This study included 15 patients with AD (Group I), 15 patients with LP (Group II), in addition to 10 healthy skin specimens served as controls (Group III). Punch biopsies were taken from lesional skin of the patients and controls for immunohistochemical detection of cathepsin L expression.

Results: Highly significant increase was found in cathepsin L expression in AD and LP patients compared to controls [P = 0.001].

Conclusion: Cathepsin L could be implicated as an important protease in the pathogenesis of AD and LP. It could be a useful marker for assessing AD severity.

No MeSH data available.


Related in: MedlinePlus

(a and b) Lichen planus showing severe (+3) expression for cathepsin L in the epidermis [line] and inflammatory cells (lymphocytes) [arrows] and endothelial cells in the dermis [arrow] (immunoperioxidase for cathepsin L ×100 and ×400, scale bar 10 μm)
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Figure 7: (a and b) Lichen planus showing severe (+3) expression for cathepsin L in the epidermis [line] and inflammatory cells (lymphocytes) [arrows] and endothelial cells in the dermis [arrow] (immunoperioxidase for cathepsin L ×100 and ×400, scale bar 10 μm)

Mentions: Expression of cathepsin L in normal skin was mainly distributed in the entire epidermis which was less than 25% and considered as negative expression [Figure 3]. Cathepsin L was detected in the cytoplasm mostly in diffuse staining pattern or somewhat condensed granular mainly in the lower spinous layers along the elongated rete ridges mainly in AD groups. In the dermis, endothelial cells of blood vessels, infiltrating inflammatory cells (lymphocytes and fibroblasts), all expressed cathepsin L in AD and in LP. In all AD patients, cathepsin L was expressed with different intensities varied from mild in 3 (20%) [Figure 4a, and b], moderate in 5 (33.3%) to severe expression in 7 (46.7%) [Figure 5a and b] [Table 3]. In all LP patients, cathepsin L was expressed with different intensities varied from mild in 8 (53.3%) [Figure 6a and b], moderate in 4 (26.7%) to severe expression in 3 (20%) [Figure 7a and b] [Table 3]. The intensity of cathepsin L expression showed statistically highly significant difference when comparing the two groups and controls (P = 0.001), while it showed no statistically significant difference when comparing between Group I and Group II with each other (P = 0.136). [Table 3]. The intensity of cathepsin L expression regarding the severity (scoring) of AD patients showed that, it was mild in 2 (25.0%), moderate in 3 (37.5%), severe in 3 (37.5%) in mild/moderate but in severe scoring it was, mild in 1 (14.3%), moderate in 2 (28.6%), marked in 4 (57.1%), (P = 0.048). There was statistically significant increase in the intensity of cathepsin L expression in patients with severe AD [Table 4].[2]


Immunohistochemical expression of cathepsin L in atopic dermatitis and lichen planus.

Ibrahim ZA, El Ashmawy AA, Abd El-Naby NM, Ghoraba HM - Indian J Dermatol (2015 Jan-Feb)

(a and b) Lichen planus showing severe (+3) expression for cathepsin L in the epidermis [line] and inflammatory cells (lymphocytes) [arrows] and endothelial cells in the dermis [arrow] (immunoperioxidase for cathepsin L ×100 and ×400, scale bar 10 μm)
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4318056&req=5

Figure 7: (a and b) Lichen planus showing severe (+3) expression for cathepsin L in the epidermis [line] and inflammatory cells (lymphocytes) [arrows] and endothelial cells in the dermis [arrow] (immunoperioxidase for cathepsin L ×100 and ×400, scale bar 10 μm)
Mentions: Expression of cathepsin L in normal skin was mainly distributed in the entire epidermis which was less than 25% and considered as negative expression [Figure 3]. Cathepsin L was detected in the cytoplasm mostly in diffuse staining pattern or somewhat condensed granular mainly in the lower spinous layers along the elongated rete ridges mainly in AD groups. In the dermis, endothelial cells of blood vessels, infiltrating inflammatory cells (lymphocytes and fibroblasts), all expressed cathepsin L in AD and in LP. In all AD patients, cathepsin L was expressed with different intensities varied from mild in 3 (20%) [Figure 4a, and b], moderate in 5 (33.3%) to severe expression in 7 (46.7%) [Figure 5a and b] [Table 3]. In all LP patients, cathepsin L was expressed with different intensities varied from mild in 8 (53.3%) [Figure 6a and b], moderate in 4 (26.7%) to severe expression in 3 (20%) [Figure 7a and b] [Table 3]. The intensity of cathepsin L expression showed statistically highly significant difference when comparing the two groups and controls (P = 0.001), while it showed no statistically significant difference when comparing between Group I and Group II with each other (P = 0.136). [Table 3]. The intensity of cathepsin L expression regarding the severity (scoring) of AD patients showed that, it was mild in 2 (25.0%), moderate in 3 (37.5%), severe in 3 (37.5%) in mild/moderate but in severe scoring it was, mild in 1 (14.3%), moderate in 2 (28.6%), marked in 4 (57.1%), (P = 0.048). There was statistically significant increase in the intensity of cathepsin L expression in patients with severe AD [Table 4].[2]

Bottom Line: Elevated expression of cathepsin L has been found in many inflammatory and neoplastic diseases.Highly significant increase was found in cathepsin L expression in AD and LP patients compared to controls [P = 0.001].It could be a useful marker for assessing AD severity.

View Article: PubMed Central - PubMed

Affiliation: Department of Dermatology and Venereology, Faculty of Medicine, Tanta University, Tanta, Gharbia, Egypt.

ABSTRACT

Background: Cathepsin L is a member of papain superfamily. It seems to promote T-cell survival, selection maturation in the thymus and enhance the antigen presentation. Cathepsin L plays an important role in tumor necrosis factors (TNF-α) induced cell death. Also it degrades the tight junction between cornedesomses in the epidermis. Elevated expression of cathepsin L has been found in many inflammatory and neoplastic diseases.

Objective: The aim of this study was to determine immunohistochemical expression of cathepsin L in atopic dermatitis (AD) and lichen planus (LP) patients in order to evaluate its role in the pathogenesis of both diseases.

Materials and methods: This study included 15 patients with AD (Group I), 15 patients with LP (Group II), in addition to 10 healthy skin specimens served as controls (Group III). Punch biopsies were taken from lesional skin of the patients and controls for immunohistochemical detection of cathepsin L expression.

Results: Highly significant increase was found in cathepsin L expression in AD and LP patients compared to controls [P = 0.001].

Conclusion: Cathepsin L could be implicated as an important protease in the pathogenesis of AD and LP. It could be a useful marker for assessing AD severity.

No MeSH data available.


Related in: MedlinePlus