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Molecular aspect on the interaction of zinc-ofloxacin complex with deoxyribonucleic acid, proposed model for binding and cytotoxicity evaluation.

Ahmadi F, Ebrahimi-Dishabi N, Mansouri K, Salimi F - Res Pharm Sci (2014 Sep-Oct)

Bottom Line: These results may introduce new drugs for chemotherapy with fewer side effects.Therefore, the interaction of OZC with DNA was investigated by using voltammetry, UV-Vis, fluorescence, FT-IR and circular dichroism spectroscopy methods, and the equilibrium binding constant (Kb), binding site size, and thermodynamic parameters were measured.The results revealed that the OZC interacts with DNA via two modes: electrostatic and outside hydrogen binding.

View Article: PubMed Central - PubMed

Affiliation: Novel Drug Delivery Research Center, Faculty of Pharmacy, Kermanshah University of Medical Sciences, Kermanshah, I.R. Iran.

ABSTRACT
Recently, several studies have shown that the metal-fluoroquinolone complexes have more antibacterial and cytotoxic effects in comparison with free fluoroquinolones. These results may introduce new drugs for chemotherapy with fewer side effects. In this work a bidentated zinc (II) complex with ofloxacin (OZC) was synthesized and cytotoxicity activities and DNA binding of the resulted complex was studied. The in-vitro anti proliferative and cytotoxic effects of the free ofloxacin (OFL) and OZC against MCF-7, CaCo2 and SKNMC cell lines were tested by using Trypan blue and lactate dehyrogenase (LDH) assay methods. Results revealed that the OZC exhibits better anti proliferative and cytotoxic activities as compared with the OFL. This may be due to the more interaction of OZC with DNA. Therefore, the interaction of OZC with DNA was investigated by using voltammetry, UV-Vis, fluorescence, FT-IR and circular dichroism spectroscopy methods, and the equilibrium binding constant (Kb), binding site size, and thermodynamic parameters were measured. The results revealed that the OZC interacts with DNA via two modes: electrostatic and outside hydrogen binding. The proposed DNA binding modes may support the greater in-vitro cytotoxicity of OZC compared to OFL alone.

No MeSH data available.


Antiproliferative effects of ofloxacin and ofloxacin-zinc complex on: a; MCF-7, b; Caco2, and c; SKNMC cell lines after 72 h treatment.
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Figure 11: Antiproliferative effects of ofloxacin and ofloxacin-zinc complex on: a; MCF-7, b; Caco2, and c; SKNMC cell lines after 72 h treatment.

Mentions: In other words the cytotoxic effects of OZC on MCF-7 cell line were considerably lower than this effect on the cell lines. To examine the influence of OFL and OZC on cancer cell proliferation, the cells were treated with increasing concentrations for 72 h. The results were shown in Figs. 10a-10c. The obtained data of counting by a Coulter counter showed that OFL and OZC induced inhibition of cell proliferation, resulting in a considerable reduction in cell growth by increasing the dose as compared with the control group.


Molecular aspect on the interaction of zinc-ofloxacin complex with deoxyribonucleic acid, proposed model for binding and cytotoxicity evaluation.

Ahmadi F, Ebrahimi-Dishabi N, Mansouri K, Salimi F - Res Pharm Sci (2014 Sep-Oct)

Antiproliferative effects of ofloxacin and ofloxacin-zinc complex on: a; MCF-7, b; Caco2, and c; SKNMC cell lines after 72 h treatment.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4318005&req=5

Figure 11: Antiproliferative effects of ofloxacin and ofloxacin-zinc complex on: a; MCF-7, b; Caco2, and c; SKNMC cell lines after 72 h treatment.
Mentions: In other words the cytotoxic effects of OZC on MCF-7 cell line were considerably lower than this effect on the cell lines. To examine the influence of OFL and OZC on cancer cell proliferation, the cells were treated with increasing concentrations for 72 h. The results were shown in Figs. 10a-10c. The obtained data of counting by a Coulter counter showed that OFL and OZC induced inhibition of cell proliferation, resulting in a considerable reduction in cell growth by increasing the dose as compared with the control group.

Bottom Line: These results may introduce new drugs for chemotherapy with fewer side effects.Therefore, the interaction of OZC with DNA was investigated by using voltammetry, UV-Vis, fluorescence, FT-IR and circular dichroism spectroscopy methods, and the equilibrium binding constant (Kb), binding site size, and thermodynamic parameters were measured.The results revealed that the OZC interacts with DNA via two modes: electrostatic and outside hydrogen binding.

View Article: PubMed Central - PubMed

Affiliation: Novel Drug Delivery Research Center, Faculty of Pharmacy, Kermanshah University of Medical Sciences, Kermanshah, I.R. Iran.

ABSTRACT
Recently, several studies have shown that the metal-fluoroquinolone complexes have more antibacterial and cytotoxic effects in comparison with free fluoroquinolones. These results may introduce new drugs for chemotherapy with fewer side effects. In this work a bidentated zinc (II) complex with ofloxacin (OZC) was synthesized and cytotoxicity activities and DNA binding of the resulted complex was studied. The in-vitro anti proliferative and cytotoxic effects of the free ofloxacin (OFL) and OZC against MCF-7, CaCo2 and SKNMC cell lines were tested by using Trypan blue and lactate dehyrogenase (LDH) assay methods. Results revealed that the OZC exhibits better anti proliferative and cytotoxic activities as compared with the OFL. This may be due to the more interaction of OZC with DNA. Therefore, the interaction of OZC with DNA was investigated by using voltammetry, UV-Vis, fluorescence, FT-IR and circular dichroism spectroscopy methods, and the equilibrium binding constant (Kb), binding site size, and thermodynamic parameters were measured. The results revealed that the OZC interacts with DNA via two modes: electrostatic and outside hydrogen binding. The proposed DNA binding modes may support the greater in-vitro cytotoxicity of OZC compared to OFL alone.

No MeSH data available.