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Antioxidant activity and safety assessment of Tragopogon buphthalmoides hydroethanolic extract: Acute and subchronic toxicities.

Mojarrab M, Mohammadi AK, Hosseinzadeh L, Siavash-Haghighi ZM - Res Pharm Sci (2014 Sep-Oct)

Bottom Line: The results of acute study indicated that the LD50 of T. buphthalmoides is higher than 2500 mg/kg.Biochemical analysis showed some significant changes including creatinine, glucose and triglyceride levels.Moreover, some significant abnormality of lung, kidney and liver organs was observed.

View Article: PubMed Central - PubMed

Affiliation: Novel Drug Delivery Research Center, Kermanshah University of Medical Sciences, Kermanshah, I.R. Iran.

ABSTRACT
Tragopogon buphthalmoides (DC.) Boiss, is widely used as a food additive with some imputed health effects in folk medicine of western Iran. Unfortunately, despite the prevalent medicinal uses of the plant, there are no reports on the toxic effects of T. buphthalmoides aerial parts. The present study evaluated the potential toxicity of dried hydroethanolic extract of the species in wistar rats. Also, we investigated antioxidant activity and total phenolic content (TPC) of the extract. In the acute study, single doses of extract were administered orally, and the rats were then monitored for 14 days. In the subchronic toxicity study, the sample was administered to the rats for 45 days. In the antioxidant capacity assays dried extract showed moderate to weak antioxidant activities. Also, the sample showed relatively notable TPC. The results of acute study indicated that the LD50 of T. buphthalmoides is higher than 2500 mg/kg. Biochemical analysis showed some significant changes including creatinine, glucose and triglyceride levels. Moreover, some significant abnormality of lung, kidney and liver organs was observed. Based on the results of this study, adverse effect level (AEL) of dried hydroethanolic extract of T. buphthalmoides considered to be less than 175 mg/kg/day for the male and female rats.

No MeSH data available.


Related in: MedlinePlus

Selected microphotographs of kidney, liver and lung hematoxylin and eosin of male rats that received the highest dose of tragopogon buphtalmoides. A; Negative control of kiney, B;Showing tubular degeneration, C; Showing protein cast in the tubules, D; Negative control liver, showing normal appearance with narrow sinusoids, E; Showing sinusoidal congestion, F; Vascular congestion, G; Showing normal appearance of lung, H; Showing increased arteriolar wall diameter I showing severe hyperaemia.
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Figure 3: Selected microphotographs of kidney, liver and lung hematoxylin and eosin of male rats that received the highest dose of tragopogon buphtalmoides. A; Negative control of kiney, B;Showing tubular degeneration, C; Showing protein cast in the tubules, D; Negative control liver, showing normal appearance with narrow sinusoids, E; Showing sinusoidal congestion, F; Vascular congestion, G; Showing normal appearance of lung, H; Showing increased arteriolar wall diameter I showing severe hyperaemia.

Mentions: The results of microscopic examination of control and the treatment animals showed hyperemia, congestion of alveolar capillaries, alveolar wall thickness in lung and congestion of sinusoidal and portal vein and accumulation of glycogen in the liver (Fig. 3). The changes in the kidney included congestion, hemorrhage and degeneration of tubules, and protein cast within tubules in the cortex. These changes were observed in the treatments but not the control animals. No histological findings in the spleen or heart could be attributed to the T. buphtalmoides.


Antioxidant activity and safety assessment of Tragopogon buphthalmoides hydroethanolic extract: Acute and subchronic toxicities.

Mojarrab M, Mohammadi AK, Hosseinzadeh L, Siavash-Haghighi ZM - Res Pharm Sci (2014 Sep-Oct)

Selected microphotographs of kidney, liver and lung hematoxylin and eosin of male rats that received the highest dose of tragopogon buphtalmoides. A; Negative control of kiney, B;Showing tubular degeneration, C; Showing protein cast in the tubules, D; Negative control liver, showing normal appearance with narrow sinusoids, E; Showing sinusoidal congestion, F; Vascular congestion, G; Showing normal appearance of lung, H; Showing increased arteriolar wall diameter I showing severe hyperaemia.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4318004&req=5

Figure 3: Selected microphotographs of kidney, liver and lung hematoxylin and eosin of male rats that received the highest dose of tragopogon buphtalmoides. A; Negative control of kiney, B;Showing tubular degeneration, C; Showing protein cast in the tubules, D; Negative control liver, showing normal appearance with narrow sinusoids, E; Showing sinusoidal congestion, F; Vascular congestion, G; Showing normal appearance of lung, H; Showing increased arteriolar wall diameter I showing severe hyperaemia.
Mentions: The results of microscopic examination of control and the treatment animals showed hyperemia, congestion of alveolar capillaries, alveolar wall thickness in lung and congestion of sinusoidal and portal vein and accumulation of glycogen in the liver (Fig. 3). The changes in the kidney included congestion, hemorrhage and degeneration of tubules, and protein cast within tubules in the cortex. These changes were observed in the treatments but not the control animals. No histological findings in the spleen or heart could be attributed to the T. buphtalmoides.

Bottom Line: The results of acute study indicated that the LD50 of T. buphthalmoides is higher than 2500 mg/kg.Biochemical analysis showed some significant changes including creatinine, glucose and triglyceride levels.Moreover, some significant abnormality of lung, kidney and liver organs was observed.

View Article: PubMed Central - PubMed

Affiliation: Novel Drug Delivery Research Center, Kermanshah University of Medical Sciences, Kermanshah, I.R. Iran.

ABSTRACT
Tragopogon buphthalmoides (DC.) Boiss, is widely used as a food additive with some imputed health effects in folk medicine of western Iran. Unfortunately, despite the prevalent medicinal uses of the plant, there are no reports on the toxic effects of T. buphthalmoides aerial parts. The present study evaluated the potential toxicity of dried hydroethanolic extract of the species in wistar rats. Also, we investigated antioxidant activity and total phenolic content (TPC) of the extract. In the acute study, single doses of extract were administered orally, and the rats were then monitored for 14 days. In the subchronic toxicity study, the sample was administered to the rats for 45 days. In the antioxidant capacity assays dried extract showed moderate to weak antioxidant activities. Also, the sample showed relatively notable TPC. The results of acute study indicated that the LD50 of T. buphthalmoides is higher than 2500 mg/kg. Biochemical analysis showed some significant changes including creatinine, glucose and triglyceride levels. Moreover, some significant abnormality of lung, kidney and liver organs was observed. Based on the results of this study, adverse effect level (AEL) of dried hydroethanolic extract of T. buphthalmoides considered to be less than 175 mg/kg/day for the male and female rats.

No MeSH data available.


Related in: MedlinePlus