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Antioxidant activity and safety assessment of Tragopogon buphthalmoides hydroethanolic extract: Acute and subchronic toxicities.

Mojarrab M, Mohammadi AK, Hosseinzadeh L, Siavash-Haghighi ZM - Res Pharm Sci (2014 Sep-Oct)

Bottom Line: The results of acute study indicated that the LD50 of T. buphthalmoides is higher than 2500 mg/kg.Biochemical analysis showed some significant changes including creatinine, glucose and triglyceride levels.Moreover, some significant abnormality of lung, kidney and liver organs was observed.

View Article: PubMed Central - PubMed

Affiliation: Novel Drug Delivery Research Center, Kermanshah University of Medical Sciences, Kermanshah, I.R. Iran.

ABSTRACT
Tragopogon buphthalmoides (DC.) Boiss, is widely used as a food additive with some imputed health effects in folk medicine of western Iran. Unfortunately, despite the prevalent medicinal uses of the plant, there are no reports on the toxic effects of T. buphthalmoides aerial parts. The present study evaluated the potential toxicity of dried hydroethanolic extract of the species in wistar rats. Also, we investigated antioxidant activity and total phenolic content (TPC) of the extract. In the acute study, single doses of extract were administered orally, and the rats were then monitored for 14 days. In the subchronic toxicity study, the sample was administered to the rats for 45 days. In the antioxidant capacity assays dried extract showed moderate to weak antioxidant activities. Also, the sample showed relatively notable TPC. The results of acute study indicated that the LD50 of T. buphthalmoides is higher than 2500 mg/kg. Biochemical analysis showed some significant changes including creatinine, glucose and triglyceride levels. Moreover, some significant abnormality of lung, kidney and liver organs was observed. Based on the results of this study, adverse effect level (AEL) of dried hydroethanolic extract of T. buphthalmoides considered to be less than 175 mg/kg/day for the male and female rats.

No MeSH data available.


Related in: MedlinePlus

Changes in females (A) and males (B) rat body weight with duration of subchronic treatment. Each point represents mean ± SEM. N=5.***p<0.05.
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Figure 2: Changes in females (A) and males (B) rat body weight with duration of subchronic treatment. Each point represents mean ± SEM. N=5.***p<0.05.

Mentions: One animal was died on the 28th day of the treatment. As shown in Fig. 2, both control and animals treated with T. buphtalmoides presented constant increase in body weight. However female rats treated with highest dose of extract showed significant increase in weight gain beginning on 28th day and continuing throughout the treatment as compared to the control. On the day 42 of the study compared to the baseline value, the control rats gained 34.42 (1.4%), while female rats treated with the 700 mg/kg gained 73.36 (2.16%). Macroscopic analysis of target organs of the treated animals (liver, heart, lung, kidneys and spleen) did not show significant changes in color and texture when compared with the control group. There were no significant differences in the organ weights between the control and the treatment groups (Table 1).


Antioxidant activity and safety assessment of Tragopogon buphthalmoides hydroethanolic extract: Acute and subchronic toxicities.

Mojarrab M, Mohammadi AK, Hosseinzadeh L, Siavash-Haghighi ZM - Res Pharm Sci (2014 Sep-Oct)

Changes in females (A) and males (B) rat body weight with duration of subchronic treatment. Each point represents mean ± SEM. N=5.***p<0.05.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4318004&req=5

Figure 2: Changes in females (A) and males (B) rat body weight with duration of subchronic treatment. Each point represents mean ± SEM. N=5.***p<0.05.
Mentions: One animal was died on the 28th day of the treatment. As shown in Fig. 2, both control and animals treated with T. buphtalmoides presented constant increase in body weight. However female rats treated with highest dose of extract showed significant increase in weight gain beginning on 28th day and continuing throughout the treatment as compared to the control. On the day 42 of the study compared to the baseline value, the control rats gained 34.42 (1.4%), while female rats treated with the 700 mg/kg gained 73.36 (2.16%). Macroscopic analysis of target organs of the treated animals (liver, heart, lung, kidneys and spleen) did not show significant changes in color and texture when compared with the control group. There were no significant differences in the organ weights between the control and the treatment groups (Table 1).

Bottom Line: The results of acute study indicated that the LD50 of T. buphthalmoides is higher than 2500 mg/kg.Biochemical analysis showed some significant changes including creatinine, glucose and triglyceride levels.Moreover, some significant abnormality of lung, kidney and liver organs was observed.

View Article: PubMed Central - PubMed

Affiliation: Novel Drug Delivery Research Center, Kermanshah University of Medical Sciences, Kermanshah, I.R. Iran.

ABSTRACT
Tragopogon buphthalmoides (DC.) Boiss, is widely used as a food additive with some imputed health effects in folk medicine of western Iran. Unfortunately, despite the prevalent medicinal uses of the plant, there are no reports on the toxic effects of T. buphthalmoides aerial parts. The present study evaluated the potential toxicity of dried hydroethanolic extract of the species in wistar rats. Also, we investigated antioxidant activity and total phenolic content (TPC) of the extract. In the acute study, single doses of extract were administered orally, and the rats were then monitored for 14 days. In the subchronic toxicity study, the sample was administered to the rats for 45 days. In the antioxidant capacity assays dried extract showed moderate to weak antioxidant activities. Also, the sample showed relatively notable TPC. The results of acute study indicated that the LD50 of T. buphthalmoides is higher than 2500 mg/kg. Biochemical analysis showed some significant changes including creatinine, glucose and triglyceride levels. Moreover, some significant abnormality of lung, kidney and liver organs was observed. Based on the results of this study, adverse effect level (AEL) of dried hydroethanolic extract of T. buphthalmoides considered to be less than 175 mg/kg/day for the male and female rats.

No MeSH data available.


Related in: MedlinePlus