Limits...
The effects of aqueous extract of Boswellia Serrata on hippocampal region CA1 and learning deficit in kindled rats.

Jalili C, Salahshoor MR, Pourmotabbed A, Moradi S, Roshankhah Sh, Darehdori AS, Motaghi M - Res Pharm Sci (2014 Sep-Oct)

Bottom Line: The aqueous extracts (0, 0.1, 0.5, 1 g/kg, i.p) are administrated to all animals for three consecutive days.Passive avoidance learning of animals is examined using shuttle box apparatus and step-through latency (STL) method.PTZ-induced kindling indicates a significant decrease in the number of pyramidal neurons and dendritic spines in hippocampal region cornu ammonis (CA1).

View Article: PubMed Central - PubMed

Affiliation: Fertility and Infertility Research Center, Kermanshah University of Medical Sciences, Kermanshah, I.R. Iran.

ABSTRACT
Temporal lobe epilepsy (TLE) is a disorder of the central nervous system in which hippocampus is mostly involved and causes memory impairment. Kindling is a model of inducing epilepsy which is created through pentylenetetrazol (PTZ) administration. This study examines the role of the aqueous extract of Boswellia on the learning and development of brain (formation of dendritic branches and axons) of the PTZ-induced kindled rats. The study is conducted on sixty-four male rats divided into 8 groups. Kindling seizures are induced by three injections of 25 mg/kg of PTZ every 15 min. The aqueous extracts (0, 0.1, 0.5, 1 g/kg, i.p) are administrated to all animals for three consecutive days. Passive avoidance learning of animals is examined using shuttle box apparatus and step-through latency (STL) method. Rats are anesthetized and their brains are fixed by transcardial perfusion method and are analyzed by morphometric methods after applying Golgi and Cresyl violet staining methods. PTZ-induced kindling indicates a significant decrease in the number of pyramidal neurons and dendritic spines in hippocampal region cornu ammonis (CA1). The STL of the kindled rats is significantly reduced compared with control ones. Also, Boswellia extract dramatically increased the number of neuronal processes in CA1 region and improves passive-avoidance learning ability in both control and PTZ-kindled animals in 1 g/kg dose administration of Boswellia extract, especially at high doses can eliminate adverse effects of seizures on cognitive function in hippocampal area CA1 in rats.

No MeSH data available.


Related in: MedlinePlus

Effect of different doses of Boswellia extract on the number of dendritic spines in CA1 region in the kindled rats. *P<0.05. A; pentylenetetrazol-saline, B; pentylenetetrazol-extract 0.1 g/kg, C; pentylenetetrazol-extract 0.5 g/kg, D; pentylenetetrazol-extract 1 g/kg (magnification 100X)
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4318003&req=5

Figure 7: Effect of different doses of Boswellia extract on the number of dendritic spines in CA1 region in the kindled rats. *P<0.05. A; pentylenetetrazol-saline, B; pentylenetetrazol-extract 0.1 g/kg, C; pentylenetetrazol-extract 0.5 g/kg, D; pentylenetetrazol-extract 1 g/kg (magnification 100X)

Mentions: When the effect of Boswellia extract on the learning ability of the saline-received (control) animals was tested, an increasing trend of step-hrough latency (STL) was found out which was significant in the group received 0.5 g/kg extract (p<0.05) (Fig. 2). The results revealed that the retention latencies of the kindled animals received Boswellia extract (0.1, 0.5, 1 g/kg) were significantly increased compared to the control group (p<0.05) (Fig. 3). Analyzing PTZ-induced kindling indicated a great decrease in the number of pyramidal neurons and dendritic spines in hippocampal region CA1 compared to control group (p<00.01) (Figs. 4 and 5). Boswellia extract significantly increased the number of neurons in CA1 region at doses of 0.5 and 1 g/kg in comparison with kindled (control) group (p<0.05) (Fig. 6). The number of neuronal dendritic spines revealed a great increase in the kindled rats (1 g/kg), due to the effect of Boswellia extract, compared to the control group (p<0.05). In other groups, however, this increase was not significant (p<0.05) (Fig. 7). Also, administration of Boswellia extract caused an increase in the number of neurons at doses of 0.5 and 1 g/kg in the healthy unkindled rats compared to control (saline) group (p<0.05). However, this increase was not remarkable in other groups (p<0.05) (Fig. 8).


The effects of aqueous extract of Boswellia Serrata on hippocampal region CA1 and learning deficit in kindled rats.

Jalili C, Salahshoor MR, Pourmotabbed A, Moradi S, Roshankhah Sh, Darehdori AS, Motaghi M - Res Pharm Sci (2014 Sep-Oct)

Effect of different doses of Boswellia extract on the number of dendritic spines in CA1 region in the kindled rats. *P<0.05. A; pentylenetetrazol-saline, B; pentylenetetrazol-extract 0.1 g/kg, C; pentylenetetrazol-extract 0.5 g/kg, D; pentylenetetrazol-extract 1 g/kg (magnification 100X)
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4318003&req=5

Figure 7: Effect of different doses of Boswellia extract on the number of dendritic spines in CA1 region in the kindled rats. *P<0.05. A; pentylenetetrazol-saline, B; pentylenetetrazol-extract 0.1 g/kg, C; pentylenetetrazol-extract 0.5 g/kg, D; pentylenetetrazol-extract 1 g/kg (magnification 100X)
Mentions: When the effect of Boswellia extract on the learning ability of the saline-received (control) animals was tested, an increasing trend of step-hrough latency (STL) was found out which was significant in the group received 0.5 g/kg extract (p<0.05) (Fig. 2). The results revealed that the retention latencies of the kindled animals received Boswellia extract (0.1, 0.5, 1 g/kg) were significantly increased compared to the control group (p<0.05) (Fig. 3). Analyzing PTZ-induced kindling indicated a great decrease in the number of pyramidal neurons and dendritic spines in hippocampal region CA1 compared to control group (p<00.01) (Figs. 4 and 5). Boswellia extract significantly increased the number of neurons in CA1 region at doses of 0.5 and 1 g/kg in comparison with kindled (control) group (p<0.05) (Fig. 6). The number of neuronal dendritic spines revealed a great increase in the kindled rats (1 g/kg), due to the effect of Boswellia extract, compared to the control group (p<0.05). In other groups, however, this increase was not significant (p<0.05) (Fig. 7). Also, administration of Boswellia extract caused an increase in the number of neurons at doses of 0.5 and 1 g/kg in the healthy unkindled rats compared to control (saline) group (p<0.05). However, this increase was not remarkable in other groups (p<0.05) (Fig. 8).

Bottom Line: The aqueous extracts (0, 0.1, 0.5, 1 g/kg, i.p) are administrated to all animals for three consecutive days.Passive avoidance learning of animals is examined using shuttle box apparatus and step-through latency (STL) method.PTZ-induced kindling indicates a significant decrease in the number of pyramidal neurons and dendritic spines in hippocampal region cornu ammonis (CA1).

View Article: PubMed Central - PubMed

Affiliation: Fertility and Infertility Research Center, Kermanshah University of Medical Sciences, Kermanshah, I.R. Iran.

ABSTRACT
Temporal lobe epilepsy (TLE) is a disorder of the central nervous system in which hippocampus is mostly involved and causes memory impairment. Kindling is a model of inducing epilepsy which is created through pentylenetetrazol (PTZ) administration. This study examines the role of the aqueous extract of Boswellia on the learning and development of brain (formation of dendritic branches and axons) of the PTZ-induced kindled rats. The study is conducted on sixty-four male rats divided into 8 groups. Kindling seizures are induced by three injections of 25 mg/kg of PTZ every 15 min. The aqueous extracts (0, 0.1, 0.5, 1 g/kg, i.p) are administrated to all animals for three consecutive days. Passive avoidance learning of animals is examined using shuttle box apparatus and step-through latency (STL) method. Rats are anesthetized and their brains are fixed by transcardial perfusion method and are analyzed by morphometric methods after applying Golgi and Cresyl violet staining methods. PTZ-induced kindling indicates a significant decrease in the number of pyramidal neurons and dendritic spines in hippocampal region cornu ammonis (CA1). The STL of the kindled rats is significantly reduced compared with control ones. Also, Boswellia extract dramatically increased the number of neuronal processes in CA1 region and improves passive-avoidance learning ability in both control and PTZ-kindled animals in 1 g/kg dose administration of Boswellia extract, especially at high doses can eliminate adverse effects of seizures on cognitive function in hippocampal area CA1 in rats.

No MeSH data available.


Related in: MedlinePlus