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Acute effect of cholecystokinin on short-term synaptic plasticity in the rat hippocampus.

Dolatabadi LK, Reisi P - Res Pharm Sci (2014 Sep-Oct)

Bottom Line: Cholecystokinin (CCK), a peptide hormone found in the gut, is the most abundant peptide neurotransmitters in the brain, and its acute effects on the brain activity have been shown.The results showed that CCK-8S has a transient excitatory effects on baseline responses, but it inhibits paired pulse indices in acute.Therefore, in a short period of time, effect of CCK on the function of synapses is time dependent, and it has stimulatory or inhibitory effects at different time periods.

View Article: PubMed Central - PubMed

Affiliation: Applied Physiology Research Center, Isfahan University of Medical Sciences, Isfahan, I.R. Iran.

ABSTRACT
Cholecystokinin (CCK), a peptide hormone found in the gut, is the most abundant peptide neurotransmitters in the brain, and its acute effects on the brain activity have been shown. In this study we aimed to evaluate the acute effects of CCK on short-term synaptic plasticity in the dentate gyrus (DG) of the rat hippocampus. Via stereotaxic surgery, the stimulating and the recording electrodes were placed in the perforant pathway and dentate gyrus, respectively and 30 min after intraperitoneal (i.p.) injection of CCK octapeptide sulfated (CCK-8S, 1.6 μg/kg), evoked responses were recorded after delivering of paired-pulse stimulations at 10 to 500 ms inter-stimulus intervals. With respect to the control group that received saline instead of CCK, in baseline responses, slope of field excitatory postsynaptic potential (fEPSP) 5 min and 10 min after injection of CCK-8S (p<0.05) and population spikes (PS)- amplitudes 5 min after injection of CCK-8S (p<0.05) were significantly increased. In paired pulse responses, PS amplitudes were increased in the CCK group, but these enhancements only were significant at inter-stimulus interval 40 ms (p<0.05). However fEPSP slopes were decreased at inter-stimulus intervals 70 ms (p<0.05), 120 ms (p<0.01), 150 ms (p<0.001) and 300 ms (p<0.001). The results showed that CCK-8S has a transient excitatory effects on baseline responses, but it inhibits paired pulse indices in acute. Therefore, in a short period of time, effect of CCK on the function of synapses is time dependent, and it has stimulatory or inhibitory effects at different time periods.

No MeSH data available.


The effect of cholecystokinin sulfated octapeptide on field excitatory postsynaptic potential in dentate gyrus of the hippocampus at A: the population spike amplitude, and B: field excitatory postsynaptic potential slope. C: single traces recorded from responses before and 5 min after the treatment. (*p<0.05 with respect to field excitatory postsynaptic potential before the treatment in cholecystokinin group. n=6 for each experimental group).
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Figure 2: The effect of cholecystokinin sulfated octapeptide on field excitatory postsynaptic potential in dentate gyrus of the hippocampus at A: the population spike amplitude, and B: field excitatory postsynaptic potential slope. C: single traces recorded from responses before and 5 min after the treatment. (*p<0.05 with respect to field excitatory postsynaptic potential before the treatment in cholecystokinin group. n=6 for each experimental group).

Mentions: In baseline responses as illustrated in Fig. 2, PS amplitude was significantly increased 5 min after injection of CCK-8S (p<0.05; Figs. 2A, 2C), but after that it backed to the initial level, so that there were no significant differences with respect to before injection during 10-30 min. In the control group injection of saline had no effects on PS amplitude (Figs. 2A, 2C).


Acute effect of cholecystokinin on short-term synaptic plasticity in the rat hippocampus.

Dolatabadi LK, Reisi P - Res Pharm Sci (2014 Sep-Oct)

The effect of cholecystokinin sulfated octapeptide on field excitatory postsynaptic potential in dentate gyrus of the hippocampus at A: the population spike amplitude, and B: field excitatory postsynaptic potential slope. C: single traces recorded from responses before and 5 min after the treatment. (*p<0.05 with respect to field excitatory postsynaptic potential before the treatment in cholecystokinin group. n=6 for each experimental group).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4318001&req=5

Figure 2: The effect of cholecystokinin sulfated octapeptide on field excitatory postsynaptic potential in dentate gyrus of the hippocampus at A: the population spike amplitude, and B: field excitatory postsynaptic potential slope. C: single traces recorded from responses before and 5 min after the treatment. (*p<0.05 with respect to field excitatory postsynaptic potential before the treatment in cholecystokinin group. n=6 for each experimental group).
Mentions: In baseline responses as illustrated in Fig. 2, PS amplitude was significantly increased 5 min after injection of CCK-8S (p<0.05; Figs. 2A, 2C), but after that it backed to the initial level, so that there were no significant differences with respect to before injection during 10-30 min. In the control group injection of saline had no effects on PS amplitude (Figs. 2A, 2C).

Bottom Line: Cholecystokinin (CCK), a peptide hormone found in the gut, is the most abundant peptide neurotransmitters in the brain, and its acute effects on the brain activity have been shown.The results showed that CCK-8S has a transient excitatory effects on baseline responses, but it inhibits paired pulse indices in acute.Therefore, in a short period of time, effect of CCK on the function of synapses is time dependent, and it has stimulatory or inhibitory effects at different time periods.

View Article: PubMed Central - PubMed

Affiliation: Applied Physiology Research Center, Isfahan University of Medical Sciences, Isfahan, I.R. Iran.

ABSTRACT
Cholecystokinin (CCK), a peptide hormone found in the gut, is the most abundant peptide neurotransmitters in the brain, and its acute effects on the brain activity have been shown. In this study we aimed to evaluate the acute effects of CCK on short-term synaptic plasticity in the dentate gyrus (DG) of the rat hippocampus. Via stereotaxic surgery, the stimulating and the recording electrodes were placed in the perforant pathway and dentate gyrus, respectively and 30 min after intraperitoneal (i.p.) injection of CCK octapeptide sulfated (CCK-8S, 1.6 μg/kg), evoked responses were recorded after delivering of paired-pulse stimulations at 10 to 500 ms inter-stimulus intervals. With respect to the control group that received saline instead of CCK, in baseline responses, slope of field excitatory postsynaptic potential (fEPSP) 5 min and 10 min after injection of CCK-8S (p<0.05) and population spikes (PS)- amplitudes 5 min after injection of CCK-8S (p<0.05) were significantly increased. In paired pulse responses, PS amplitudes were increased in the CCK group, but these enhancements only were significant at inter-stimulus interval 40 ms (p<0.05). However fEPSP slopes were decreased at inter-stimulus intervals 70 ms (p<0.05), 120 ms (p<0.01), 150 ms (p<0.001) and 300 ms (p<0.001). The results showed that CCK-8S has a transient excitatory effects on baseline responses, but it inhibits paired pulse indices in acute. Therefore, in a short period of time, effect of CCK on the function of synapses is time dependent, and it has stimulatory or inhibitory effects at different time periods.

No MeSH data available.