Limits...
Caffeic acid derivatives from Bupleurum chinense.

Haghi G, Hatami A, Mehran M, Hosseini H - Res Pharm Sci (2014 Sep-Oct)

Bottom Line: In addition, the flavonoid aglycones were characterized and quantified after acid hydrolysis of the plant material.The flavonols profile showed quercetin (0.36 g per 100 g), kaempferol (1.11 g per 100 g) and isorhamnetin (0.16 g per 100 g).Total phenolic and total flavonoid contents ranged from 7.3 to 18.7% and 0.58 to 2.72% in dry plant material, respectively.

View Article: PubMed Central - PubMed

Affiliation: Phytochemistry Group, Barij Essence Medicinal Plants Research Center, Kashan, I.R. Iran.

ABSTRACT
In this study, caffeic acid (CA) and its three derivatives including 3-caffeoylquinic acid (3-CQA, neochlorogenic acid), 4-caffeoylquinic acid (4-CQA, cryptochlorogenic acid), and 5-caffeoylquinic acid (5-CQA, chlorogenic acid) were identified in Bupleurum chinense aerial parts using reverse-phase high-performance liquid chromatography (RP-HPLC) with photodiode array (PDA) detector, reference compounds and chemical reactions. Separation was performed on a C18 column using gradient elution with 4% (v/v) aqueous acetic acid and acetonitrile as mobile phase at ambient temperature. In addition, the flavonoid aglycones were characterized and quantified after acid hydrolysis of the plant material. The flavonols profile showed quercetin (0.36 g per 100 g), kaempferol (1.11 g per 100 g) and isorhamnetin (0.16 g per 100 g). Total phenolic and total flavonoid contents ranged from 7.3 to 18.7% and 0.58 to 2.72% in dry plant material, respectively.

No MeSH data available.


Related in: MedlinePlus

High-performance liquid chromatography chromatograms of standard materials (a); methanol/water (70/30 v/v) extract (b); 1:3-caffeoylquinic acid, 2:4-caffeoylquinic acid, 3: 5-caffeoylquinic acid, 4:caffeic acid.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4318000&req=5

Figure 2: High-performance liquid chromatography chromatograms of standard materials (a); methanol/water (70/30 v/v) extract (b); 1:3-caffeoylquinic acid, 2:4-caffeoylquinic acid, 3: 5-caffeoylquinic acid, 4:caffeic acid.

Mentions: A methanol/water (70/30 V/V) extract from the dried aerial parts of B. chinense was analyzed by RP-HPLC to characterize the chemical structure of CA acid and its derivatives. Four compounds were identified as CA, 3-CQA, 4-CQA and 5-CQA (Figs. 2a and 2b). The recorded UV spectra by PDA detector, elution sequence of peaks, and retention times in chromatogram of the crude extract were the same as chromatogram of their standards and comparison with recorded data (30,40). UV spectra of peaks 1-4 recorded by PDA detector were identical to the UV spectra of CA and its derivatives (λmax at approx. 240, 300sh, 328 nm) standards. Some authors have named CA derivatives pre-IUPAC numbering (35). Increasing the standard solutions to the crude extract increased the peak area of analytes (peaks 1-4). The chromatograms of standard materials and the crude methanol/water (70/30 V/V) extract have been exhibited in Figs. 2a and 2b. The crude extract was subjected hydrolyse and isomerization. The chromatograms of 5-CQA and the extract isomerized and hydrolyzed have been showed in Fig. 3. The hydrolysis process showed the presence of quercetin, kaempferol and isorhamnetin in dry plant material. Figs. 4a and 4b exhibit the chromatograms of standard materials and acid hydrolysis of plant sample.


Caffeic acid derivatives from Bupleurum chinense.

Haghi G, Hatami A, Mehran M, Hosseini H - Res Pharm Sci (2014 Sep-Oct)

High-performance liquid chromatography chromatograms of standard materials (a); methanol/water (70/30 v/v) extract (b); 1:3-caffeoylquinic acid, 2:4-caffeoylquinic acid, 3: 5-caffeoylquinic acid, 4:caffeic acid.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4318000&req=5

Figure 2: High-performance liquid chromatography chromatograms of standard materials (a); methanol/water (70/30 v/v) extract (b); 1:3-caffeoylquinic acid, 2:4-caffeoylquinic acid, 3: 5-caffeoylquinic acid, 4:caffeic acid.
Mentions: A methanol/water (70/30 V/V) extract from the dried aerial parts of B. chinense was analyzed by RP-HPLC to characterize the chemical structure of CA acid and its derivatives. Four compounds were identified as CA, 3-CQA, 4-CQA and 5-CQA (Figs. 2a and 2b). The recorded UV spectra by PDA detector, elution sequence of peaks, and retention times in chromatogram of the crude extract were the same as chromatogram of their standards and comparison with recorded data (30,40). UV spectra of peaks 1-4 recorded by PDA detector were identical to the UV spectra of CA and its derivatives (λmax at approx. 240, 300sh, 328 nm) standards. Some authors have named CA derivatives pre-IUPAC numbering (35). Increasing the standard solutions to the crude extract increased the peak area of analytes (peaks 1-4). The chromatograms of standard materials and the crude methanol/water (70/30 V/V) extract have been exhibited in Figs. 2a and 2b. The crude extract was subjected hydrolyse and isomerization. The chromatograms of 5-CQA and the extract isomerized and hydrolyzed have been showed in Fig. 3. The hydrolysis process showed the presence of quercetin, kaempferol and isorhamnetin in dry plant material. Figs. 4a and 4b exhibit the chromatograms of standard materials and acid hydrolysis of plant sample.

Bottom Line: In addition, the flavonoid aglycones were characterized and quantified after acid hydrolysis of the plant material.The flavonols profile showed quercetin (0.36 g per 100 g), kaempferol (1.11 g per 100 g) and isorhamnetin (0.16 g per 100 g).Total phenolic and total flavonoid contents ranged from 7.3 to 18.7% and 0.58 to 2.72% in dry plant material, respectively.

View Article: PubMed Central - PubMed

Affiliation: Phytochemistry Group, Barij Essence Medicinal Plants Research Center, Kashan, I.R. Iran.

ABSTRACT
In this study, caffeic acid (CA) and its three derivatives including 3-caffeoylquinic acid (3-CQA, neochlorogenic acid), 4-caffeoylquinic acid (4-CQA, cryptochlorogenic acid), and 5-caffeoylquinic acid (5-CQA, chlorogenic acid) were identified in Bupleurum chinense aerial parts using reverse-phase high-performance liquid chromatography (RP-HPLC) with photodiode array (PDA) detector, reference compounds and chemical reactions. Separation was performed on a C18 column using gradient elution with 4% (v/v) aqueous acetic acid and acetonitrile as mobile phase at ambient temperature. In addition, the flavonoid aglycones were characterized and quantified after acid hydrolysis of the plant material. The flavonols profile showed quercetin (0.36 g per 100 g), kaempferol (1.11 g per 100 g) and isorhamnetin (0.16 g per 100 g). Total phenolic and total flavonoid contents ranged from 7.3 to 18.7% and 0.58 to 2.72% in dry plant material, respectively.

No MeSH data available.


Related in: MedlinePlus