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Suprabasin as a novel tumor endothelial cell marker.

Alam MT, Nagao-Kitamoto H, Ohga N, Akiyama K, Maishi N, Kawamoto T, Shinohara N, Taketomi A, Shindoh M, Hida Y, Hida K - Cancer Sci. (2014)

Bottom Line: SBSN knockdown inhibited the migration and tube formation ability of TEC.We also showed that the AKT pathway was a downstream factor of SBSN.These findings suggest that SBSN is involved in the angiogenic potential of TEC and may be a novel TEC marker.

View Article: PubMed Central - PubMed

Affiliation: Vascular Biology, Frontier Research Unit, Institute for Genetic Medicine, Hokkaido University, Sapporo, Japan; Department of Oral Pathology and Biology, Graduate School of Dental Medicine, Hokkaido University, Sapporo, Japan.

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Suprabasin (SBSN) expression in human tumor endothelial cells (hTEC). (a, b) Relative SBSN mRNA expression levels in hNEC and hTEC evaluated by quantitative PCR (a, RCC, n = 4; b, colon tumor, n = 2). *P < 0.01 versus control; two-sided Student's t-test. (c, d) Clinical samples of renal cell carcinoma (RCC) and colon cancer-derived tumor endothelial cells were double-stained with anti-CD31 and anti-SBSN antibodies. Scale bar: 50 μm.
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fig01: Suprabasin (SBSN) expression in human tumor endothelial cells (hTEC). (a, b) Relative SBSN mRNA expression levels in hNEC and hTEC evaluated by quantitative PCR (a, RCC, n = 4; b, colon tumor, n = 2). *P < 0.01 versus control; two-sided Student's t-test. (c, d) Clinical samples of renal cell carcinoma (RCC) and colon cancer-derived tumor endothelial cells were double-stained with anti-CD31 and anti-SBSN antibodies. Scale bar: 50 μm.

Mentions: To analyze the SBSN expression in hTEC and hNEC, we isolated hTEC from tissues of four cases of RCC and two cases of colon cancer. Furthermore, hNEC were isolated from the tissues of normal renal tissue and colon in the same patients.(14,28) The SBSN mRNA expression levels in hTEC isolated from RCC and colon cancer tissues were higher than those of hNEC (Fig. 1a,b). Double-immunofluorescence staining with anti-SBSN and anti-CD31 antibodies revealed that SBSN was markedly expressed in tumor blood vessels both in RCC and colon cancer, whereas the SBSN expression was low in normal blood vessels (Fig. 1c,d). In addition, SBSN mRNA expression levels were higher in human renal tumor tissues than those in normal tissues (Suppl. Fig. S1). These findings showed that SBSN was upregulated in hTEC from several tumor types.


Suprabasin as a novel tumor endothelial cell marker.

Alam MT, Nagao-Kitamoto H, Ohga N, Akiyama K, Maishi N, Kawamoto T, Shinohara N, Taketomi A, Shindoh M, Hida Y, Hida K - Cancer Sci. (2014)

Suprabasin (SBSN) expression in human tumor endothelial cells (hTEC). (a, b) Relative SBSN mRNA expression levels in hNEC and hTEC evaluated by quantitative PCR (a, RCC, n = 4; b, colon tumor, n = 2). *P < 0.01 versus control; two-sided Student's t-test. (c, d) Clinical samples of renal cell carcinoma (RCC) and colon cancer-derived tumor endothelial cells were double-stained with anti-CD31 and anti-SBSN antibodies. Scale bar: 50 μm.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4317965&req=5

fig01: Suprabasin (SBSN) expression in human tumor endothelial cells (hTEC). (a, b) Relative SBSN mRNA expression levels in hNEC and hTEC evaluated by quantitative PCR (a, RCC, n = 4; b, colon tumor, n = 2). *P < 0.01 versus control; two-sided Student's t-test. (c, d) Clinical samples of renal cell carcinoma (RCC) and colon cancer-derived tumor endothelial cells were double-stained with anti-CD31 and anti-SBSN antibodies. Scale bar: 50 μm.
Mentions: To analyze the SBSN expression in hTEC and hNEC, we isolated hTEC from tissues of four cases of RCC and two cases of colon cancer. Furthermore, hNEC were isolated from the tissues of normal renal tissue and colon in the same patients.(14,28) The SBSN mRNA expression levels in hTEC isolated from RCC and colon cancer tissues were higher than those of hNEC (Fig. 1a,b). Double-immunofluorescence staining with anti-SBSN and anti-CD31 antibodies revealed that SBSN was markedly expressed in tumor blood vessels both in RCC and colon cancer, whereas the SBSN expression was low in normal blood vessels (Fig. 1c,d). In addition, SBSN mRNA expression levels were higher in human renal tumor tissues than those in normal tissues (Suppl. Fig. S1). These findings showed that SBSN was upregulated in hTEC from several tumor types.

Bottom Line: SBSN knockdown inhibited the migration and tube formation ability of TEC.We also showed that the AKT pathway was a downstream factor of SBSN.These findings suggest that SBSN is involved in the angiogenic potential of TEC and may be a novel TEC marker.

View Article: PubMed Central - PubMed

Affiliation: Vascular Biology, Frontier Research Unit, Institute for Genetic Medicine, Hokkaido University, Sapporo, Japan; Department of Oral Pathology and Biology, Graduate School of Dental Medicine, Hokkaido University, Sapporo, Japan.

Show MeSH
Related in: MedlinePlus