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Photodynamic therapy using nanoparticle loaded with indocyanine green for experimental peritoneal dissemination of gastric cancer.

Tsujimoto H, Morimoto Y, Takahata R, Nomura S, Yoshida K, Horiguchi H, Hiraki S, Ono S, Miyazaki H, Saito D, Hara I, Ozeki E, Yamamoto J, Hase K - Cancer Sci. (2014)

Bottom Line: Although there have been multiple advances in the development of novel anticancer agents and operative procedures, prognosis of patients with advanced gastric cancer remains poor, especially in patients with peritoneal metastasis.Forty-eight hours after injection of the photosensitizer, in vivo and ex vivo imaging was carried out.In conclusion, ICGm can be used as a novel diagnostic and therapeutic nanodevice in peritoneal dissemination of gastric cancer.

View Article: PubMed Central - PubMed

Affiliation: Department of Surgery, National Defense Medical College, Tokorozawa, Japan.

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Related in: MedlinePlus

Luminescence and fluorescence imaging of mice with peritoneal dissemination. Luminescence originating from tumors is seen through the abdominal wall as well as in the post-laparotomy abdominal cavity in both treatment groups. No specific fluorescence is seen in indocyanine green (ICG)-treated mice (a), whereas obvious fluorescence signals identical to luminescence sites are seen through the abdominal wall and in the post-laparotomy abdominal cavity in ICG loaded lactosome (ICGm)-treated mice (b). Relative light units/pixel are indicated as color scale bars.
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fig02: Luminescence and fluorescence imaging of mice with peritoneal dissemination. Luminescence originating from tumors is seen through the abdominal wall as well as in the post-laparotomy abdominal cavity in both treatment groups. No specific fluorescence is seen in indocyanine green (ICG)-treated mice (a), whereas obvious fluorescence signals identical to luminescence sites are seen through the abdominal wall and in the post-laparotomy abdominal cavity in ICG loaded lactosome (ICGm)-treated mice (b). Relative light units/pixel are indicated as color scale bars.

Mentions: We detected luminescent signals through the abdominal wall as well as in the post-laparotomy abdominal cavity in both treatment groups (Fig. 2). Fluorescence imaging revealed that no specific fluorescence was observed in ICG-treated mice through the abdominal wall or post-laparotomy abdominal cavity (Fig. 2a), whereas we detected fluorescence in the location identical to the site of luminescence in ICGm-treated mice (Fig. 2b). Although we detected luminescent signals in disseminated nodules in both groups, disseminated nodules in ICGm-treated mice, but not ICG-treated mice, were visualized in ex vivo fluorescence imaging (Fig. 3).


Photodynamic therapy using nanoparticle loaded with indocyanine green for experimental peritoneal dissemination of gastric cancer.

Tsujimoto H, Morimoto Y, Takahata R, Nomura S, Yoshida K, Horiguchi H, Hiraki S, Ono S, Miyazaki H, Saito D, Hara I, Ozeki E, Yamamoto J, Hase K - Cancer Sci. (2014)

Luminescence and fluorescence imaging of mice with peritoneal dissemination. Luminescence originating from tumors is seen through the abdominal wall as well as in the post-laparotomy abdominal cavity in both treatment groups. No specific fluorescence is seen in indocyanine green (ICG)-treated mice (a), whereas obvious fluorescence signals identical to luminescence sites are seen through the abdominal wall and in the post-laparotomy abdominal cavity in ICG loaded lactosome (ICGm)-treated mice (b). Relative light units/pixel are indicated as color scale bars.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4317961&req=5

fig02: Luminescence and fluorescence imaging of mice with peritoneal dissemination. Luminescence originating from tumors is seen through the abdominal wall as well as in the post-laparotomy abdominal cavity in both treatment groups. No specific fluorescence is seen in indocyanine green (ICG)-treated mice (a), whereas obvious fluorescence signals identical to luminescence sites are seen through the abdominal wall and in the post-laparotomy abdominal cavity in ICG loaded lactosome (ICGm)-treated mice (b). Relative light units/pixel are indicated as color scale bars.
Mentions: We detected luminescent signals through the abdominal wall as well as in the post-laparotomy abdominal cavity in both treatment groups (Fig. 2). Fluorescence imaging revealed that no specific fluorescence was observed in ICG-treated mice through the abdominal wall or post-laparotomy abdominal cavity (Fig. 2a), whereas we detected fluorescence in the location identical to the site of luminescence in ICGm-treated mice (Fig. 2b). Although we detected luminescent signals in disseminated nodules in both groups, disseminated nodules in ICGm-treated mice, but not ICG-treated mice, were visualized in ex vivo fluorescence imaging (Fig. 3).

Bottom Line: Although there have been multiple advances in the development of novel anticancer agents and operative procedures, prognosis of patients with advanced gastric cancer remains poor, especially in patients with peritoneal metastasis.Forty-eight hours after injection of the photosensitizer, in vivo and ex vivo imaging was carried out.In conclusion, ICGm can be used as a novel diagnostic and therapeutic nanodevice in peritoneal dissemination of gastric cancer.

View Article: PubMed Central - PubMed

Affiliation: Department of Surgery, National Defense Medical College, Tokorozawa, Japan.

Show MeSH
Related in: MedlinePlus