Photodynamic therapy using nanoparticle loaded with indocyanine green for experimental peritoneal dissemination of gastric cancer.
Bottom Line: Forty-eight hours after injection of the photosensitizer, in vivo and ex vivo imaging was carried out.The PDT reduced the total weight of the disseminated nodules and significantly improved weight loss and survival rate in ICGm-treated mice.In conclusion, ICGm can be used as a novel diagnostic and therapeutic nanodevice in peritoneal dissemination of gastric cancer.
Affiliation: Department of Surgery, National Defense Medical College, Tokorozawa, Japan.Show MeSH
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Mentions: Chloroform solutions of PS–PLLA and ICG–PLAA were mixed at a ratio of 0.78:0.22. The solvent was removed under a reduced pressure environment and the formed thin film was dissolved in 10 mM Tris–HCl buffer (pH 7.4). The resulting aqueous solution was purified by Sephacryl S-100 size exclusion chromatography by elution with 10 mM Tris-HCl buffer (pH 7.4) to obtain ICGm (Fig. 1). Dynamic light-scattering analysis revealed that the hydrodynamic diameter of ICGm was 40–50 nm and the zeta potential of ICGm was −0.51 mV (Fig. S1).
Affiliation: Department of Surgery, National Defense Medical College, Tokorozawa, Japan.