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Photodynamic therapy using nanoparticle loaded with indocyanine green for experimental peritoneal dissemination of gastric cancer.

Tsujimoto H, Morimoto Y, Takahata R, Nomura S, Yoshida K, Horiguchi H, Hiraki S, Ono S, Miyazaki H, Saito D, Hara I, Ozeki E, Yamamoto J, Hase K - Cancer Sci. (2014)

Bottom Line: Although there have been multiple advances in the development of novel anticancer agents and operative procedures, prognosis of patients with advanced gastric cancer remains poor, especially in patients with peritoneal metastasis.Forty-eight hours after injection of the photosensitizer, in vivo and ex vivo imaging was carried out.In conclusion, ICGm can be used as a novel diagnostic and therapeutic nanodevice in peritoneal dissemination of gastric cancer.

View Article: PubMed Central - PubMed

Affiliation: Department of Surgery, National Defense Medical College, Tokorozawa, Japan.

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Structure of indocyanine green (ICG) loaded lactosome (ICGm). ICGm is a molecular assembly composed of hydrophobic helical poly(L-lactic acid) (PLLA) and hydrophilic poly(sarcosine) (PS) amphiphilic block polydepsipeptide including ICG-labeled PLLA in the hydrophobic inner core.
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fig01: Structure of indocyanine green (ICG) loaded lactosome (ICGm). ICGm is a molecular assembly composed of hydrophobic helical poly(L-lactic acid) (PLLA) and hydrophilic poly(sarcosine) (PS) amphiphilic block polydepsipeptide including ICG-labeled PLLA in the hydrophobic inner core.

Mentions: Chloroform solutions of PS–PLLA and ICG–PLAA were mixed at a ratio of 0.78:0.22. The solvent was removed under a reduced pressure environment and the formed thin film was dissolved in 10 mM Tris–HCl buffer (pH 7.4). The resulting aqueous solution was purified by Sephacryl S-100 size exclusion chromatography by elution with 10 mM Tris-HCl buffer (pH 7.4) to obtain ICGm (Fig. 1). Dynamic light-scattering analysis revealed that the hydrodynamic diameter of ICGm was 40–50 nm and the zeta potential of ICGm was −0.51 mV (Fig. S1).


Photodynamic therapy using nanoparticle loaded with indocyanine green for experimental peritoneal dissemination of gastric cancer.

Tsujimoto H, Morimoto Y, Takahata R, Nomura S, Yoshida K, Horiguchi H, Hiraki S, Ono S, Miyazaki H, Saito D, Hara I, Ozeki E, Yamamoto J, Hase K - Cancer Sci. (2014)

Structure of indocyanine green (ICG) loaded lactosome (ICGm). ICGm is a molecular assembly composed of hydrophobic helical poly(L-lactic acid) (PLLA) and hydrophilic poly(sarcosine) (PS) amphiphilic block polydepsipeptide including ICG-labeled PLLA in the hydrophobic inner core.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4317961&req=5

fig01: Structure of indocyanine green (ICG) loaded lactosome (ICGm). ICGm is a molecular assembly composed of hydrophobic helical poly(L-lactic acid) (PLLA) and hydrophilic poly(sarcosine) (PS) amphiphilic block polydepsipeptide including ICG-labeled PLLA in the hydrophobic inner core.
Mentions: Chloroform solutions of PS–PLLA and ICG–PLAA were mixed at a ratio of 0.78:0.22. The solvent was removed under a reduced pressure environment and the formed thin film was dissolved in 10 mM Tris–HCl buffer (pH 7.4). The resulting aqueous solution was purified by Sephacryl S-100 size exclusion chromatography by elution with 10 mM Tris-HCl buffer (pH 7.4) to obtain ICGm (Fig. 1). Dynamic light-scattering analysis revealed that the hydrodynamic diameter of ICGm was 40–50 nm and the zeta potential of ICGm was −0.51 mV (Fig. S1).

Bottom Line: Although there have been multiple advances in the development of novel anticancer agents and operative procedures, prognosis of patients with advanced gastric cancer remains poor, especially in patients with peritoneal metastasis.Forty-eight hours after injection of the photosensitizer, in vivo and ex vivo imaging was carried out.In conclusion, ICGm can be used as a novel diagnostic and therapeutic nanodevice in peritoneal dissemination of gastric cancer.

View Article: PubMed Central - PubMed

Affiliation: Department of Surgery, National Defense Medical College, Tokorozawa, Japan.

Show MeSH
Related in: MedlinePlus