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miR-185-3p regulates nasopharyngeal carcinoma radioresistance by targeting WNT2B in vitro.

Li G, Wang Y, Liu Y, Su Z, Liu C, Ren S, Deng T, Huang D, Tian Y, Qiu Y - Cancer Sci. (2014)

Bottom Line: Luciferase reporter assays confirmed that miR-185-3p directly targeted the coding region of WNT2B.Furthermore, we found radioresistance decreased in WNT2B-silenced NPC cells.We concluded that miR-185-3p contributed to the radioresistance of NPC via modulation of WNT2B expression in vitro.

View Article: PubMed Central - PubMed

Affiliation: Department of Otolaryngology Head and Neck Surgery, Xiangya Hospital, Central South University, Changsha, China; Otolaryngology Major Disease Research Key Laboratory of Hunan Province, Changsha, Hunan, China.

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Inhibition of miR-185-3p in 5-8F cells increases their radioresistance. (a) Transfection efficiency was determined under a fluorescent microscope. (b) MiR-185-3p downregulated expression in transfected 5-8F cells. (c) Survival rates for different cell groups were examined using CCK-8 assays after irradiation. (d) A representative image of colony formation in different cell groups with or without irradiation exposure. The results are the average of three independent experiments ± standard deviation (**P < 0.01).
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fig03: Inhibition of miR-185-3p in 5-8F cells increases their radioresistance. (a) Transfection efficiency was determined under a fluorescent microscope. (b) MiR-185-3p downregulated expression in transfected 5-8F cells. (c) Survival rates for different cell groups were examined using CCK-8 assays after irradiation. (d) A representative image of colony formation in different cell groups with or without irradiation exposure. The results are the average of three independent experiments ± standard deviation (**P < 0.01).

Mentions: Following overexpression of miR-185-3p in CNE-2 cells, we then suppressed the expression of miR-185-3p in 5-8F cells. Our data revealed that the transfection efficiency was 95.1 ± 4.0% and miR-185-3p was successfully inhibited in 5-8F cells (13.6 ± 2.6%; P < 0.01; Fig. 3a,b). The 5-8F cells with less miR-185-3p had a higher survival capacity following 6 Gy irradiation (P < 0.05; Fig. 3c). At the same time, the number of surviving clones was significantly increased and the size of colonies was larger compared with the control 5-8F cells (72.1 ± 15.2% vs 40.5 ± 6.7%; P < 0.05; Fig. 3d). Taken together, these data confirmed that miR-185-3p could increase the radioresistance of NPC cells to irradiation.


miR-185-3p regulates nasopharyngeal carcinoma radioresistance by targeting WNT2B in vitro.

Li G, Wang Y, Liu Y, Su Z, Liu C, Ren S, Deng T, Huang D, Tian Y, Qiu Y - Cancer Sci. (2014)

Inhibition of miR-185-3p in 5-8F cells increases their radioresistance. (a) Transfection efficiency was determined under a fluorescent microscope. (b) MiR-185-3p downregulated expression in transfected 5-8F cells. (c) Survival rates for different cell groups were examined using CCK-8 assays after irradiation. (d) A representative image of colony formation in different cell groups with or without irradiation exposure. The results are the average of three independent experiments ± standard deviation (**P < 0.01).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4317952&req=5

fig03: Inhibition of miR-185-3p in 5-8F cells increases their radioresistance. (a) Transfection efficiency was determined under a fluorescent microscope. (b) MiR-185-3p downregulated expression in transfected 5-8F cells. (c) Survival rates for different cell groups were examined using CCK-8 assays after irradiation. (d) A representative image of colony formation in different cell groups with or without irradiation exposure. The results are the average of three independent experiments ± standard deviation (**P < 0.01).
Mentions: Following overexpression of miR-185-3p in CNE-2 cells, we then suppressed the expression of miR-185-3p in 5-8F cells. Our data revealed that the transfection efficiency was 95.1 ± 4.0% and miR-185-3p was successfully inhibited in 5-8F cells (13.6 ± 2.6%; P < 0.01; Fig. 3a,b). The 5-8F cells with less miR-185-3p had a higher survival capacity following 6 Gy irradiation (P < 0.05; Fig. 3c). At the same time, the number of surviving clones was significantly increased and the size of colonies was larger compared with the control 5-8F cells (72.1 ± 15.2% vs 40.5 ± 6.7%; P < 0.05; Fig. 3d). Taken together, these data confirmed that miR-185-3p could increase the radioresistance of NPC cells to irradiation.

Bottom Line: Luciferase reporter assays confirmed that miR-185-3p directly targeted the coding region of WNT2B.Furthermore, we found radioresistance decreased in WNT2B-silenced NPC cells.We concluded that miR-185-3p contributed to the radioresistance of NPC via modulation of WNT2B expression in vitro.

View Article: PubMed Central - PubMed

Affiliation: Department of Otolaryngology Head and Neck Surgery, Xiangya Hospital, Central South University, Changsha, China; Otolaryngology Major Disease Research Key Laboratory of Hunan Province, Changsha, Hunan, China.

Show MeSH
Related in: MedlinePlus