Anti-proliferative and apoptosis-inducing activity of lycopene against three subtypes of human breast cancer cell lines.
Bottom Line: Lycopene treatment for 168 consecutive hours exhibited a time-dependent and dose-dependent anti-proliferative activity against these cell lines by arresting the cell cycle at the G0 /G1 phase at physiologically achievable concentrations found in human plasma.The greatest growth inhibition was observed in MDA-MB-468 where the sub-G0 /G1 apoptotic population was significantly increased, with demonstrable cleavage of PARP.In triple negative cells, lycopene inhibited the phosphorylation of Akt and its downstream molecule mTOR, followed by subsequent upregulation of proapoptotic Bax without affecting anti-apoptotic Bcl-xL.
Affiliation: Graduate School of Health and Nutritional Sciences, Nakamura Gakuen University, Fukuoka, Japan.Show MeSH
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Mentions: Lycopene treatment for 168 consecutive hours exhibited dose-dependent growth inhibitory activities against MCF-7, SK-BR-3 and MDA-MB-468 cells, with the greatest growth inhibition observed in MDA-MB-468 (Fig.1). MDA-MB-468 cells treated with lycopene for 168 h showed significantly lower IC50 value (10.3 μM), compared to MCF-7 cells (29.9 μM) and SK-BR-3 (22.8 μM), being threefold more sensitive to lycopene than MCF-7 cells (Table1). HER2-positive cells were moderately sensitive to lycopene, showing the IC50 between those of triple-negative and hormone-receptor positive cells. When the exposure time was shortened, IC50 values increased and there were no significant differences of lycopene sensitivity between these cell lines at 72-h exposure (Table1). These data indicate that a relatively long time (more than 72 h) would be required for lycopene to exhibit its true anti-proliferative activity for individual cell lines.
Affiliation: Graduate School of Health and Nutritional Sciences, Nakamura Gakuen University, Fukuoka, Japan.