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Hedgehog signaling pathway is a potential therapeutic target for gallbladder cancer.

Matsushita S, Onishi H, Nakano K, Nagamatsu I, Imaizumi A, Hattori M, Oda Y, Tanaka M, Katano M - Cancer Sci. (2014)

Bottom Line: New effective therapeutic strategies are greatly needed.In contrast, inhibiting the effector Smo decreased the anchor-dependent and anchor-independent proliferation.These results suggest that Hh signaling is elevated in GBC and may be involved in the acquisition of malignant phenotypes, and that Hh signaling may be a potential therapeutic target for GBC.

View Article: PubMed Central - PubMed

Affiliation: Department of Cancer Therapy and Research, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan.

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Related in: MedlinePlus

Expression of Hedgehog (Hh) components in gallbladder cancer (GBC) cells is also evident at mRNA and protein levels. (a) Total RNA from GBC cells (GBd15, TGBC2TKB) was reverse transcribed and the cDNA was subjected to conventional RT-PCR for the indicated mRNAs. GAPDH was used as control. (b) Protein extracted from GBC cells (GBd15, TGBC2TKB) was subjected to Western blot analysis for Hh components. Tubulin was used as the loading control. Shh, Sonic Hh; Smo, Smoothened.
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fig02: Expression of Hedgehog (Hh) components in gallbladder cancer (GBC) cells is also evident at mRNA and protein levels. (a) Total RNA from GBC cells (GBd15, TGBC2TKB) was reverse transcribed and the cDNA was subjected to conventional RT-PCR for the indicated mRNAs. GAPDH was used as control. (b) Protein extracted from GBC cells (GBd15, TGBC2TKB) was subjected to Western blot analysis for Hh components. Tubulin was used as the loading control. Shh, Sonic Hh; Smo, Smoothened.

Mentions: We used GBC cell lines to characterize Hh signaling in vitro. As shown in Figure2, mRNA and protein products for of Gli1, Shh, and Smo were detected in GBd15 and TGBC2TKB cells.


Hedgehog signaling pathway is a potential therapeutic target for gallbladder cancer.

Matsushita S, Onishi H, Nakano K, Nagamatsu I, Imaizumi A, Hattori M, Oda Y, Tanaka M, Katano M - Cancer Sci. (2014)

Expression of Hedgehog (Hh) components in gallbladder cancer (GBC) cells is also evident at mRNA and protein levels. (a) Total RNA from GBC cells (GBd15, TGBC2TKB) was reverse transcribed and the cDNA was subjected to conventional RT-PCR for the indicated mRNAs. GAPDH was used as control. (b) Protein extracted from GBC cells (GBd15, TGBC2TKB) was subjected to Western blot analysis for Hh components. Tubulin was used as the loading control. Shh, Sonic Hh; Smo, Smoothened.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4317941&req=5

fig02: Expression of Hedgehog (Hh) components in gallbladder cancer (GBC) cells is also evident at mRNA and protein levels. (a) Total RNA from GBC cells (GBd15, TGBC2TKB) was reverse transcribed and the cDNA was subjected to conventional RT-PCR for the indicated mRNAs. GAPDH was used as control. (b) Protein extracted from GBC cells (GBd15, TGBC2TKB) was subjected to Western blot analysis for Hh components. Tubulin was used as the loading control. Shh, Sonic Hh; Smo, Smoothened.
Mentions: We used GBC cell lines to characterize Hh signaling in vitro. As shown in Figure2, mRNA and protein products for of Gli1, Shh, and Smo were detected in GBd15 and TGBC2TKB cells.

Bottom Line: New effective therapeutic strategies are greatly needed.In contrast, inhibiting the effector Smo decreased the anchor-dependent and anchor-independent proliferation.These results suggest that Hh signaling is elevated in GBC and may be involved in the acquisition of malignant phenotypes, and that Hh signaling may be a potential therapeutic target for GBC.

View Article: PubMed Central - PubMed

Affiliation: Department of Cancer Therapy and Research, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan.

Show MeSH
Related in: MedlinePlus