MicroRNA-16 inhibits glioma cell growth and invasion through suppression of BCL2 and the nuclear factor-κB1/MMP9 signaling pathway.
Bottom Line: MicroRNA-16 decreased glioma malignancy by downregulating NF-κB1 and MMP9, and led to suppressed invasiveness of human glioma cell lines SHG44, U87, and U373.Our results also indicated that upregulation of miR-16 promoted apoptosis by suppressing BCL2 expression.Finally, the upregulation of miR-16 in a nude mice model of human glioma resulted in significant suppression of glioma growth and invasiveness.
Affiliation: Department of Neurosurgery and Brain and Nerve Research Laboratory, The First Affiliated Hospital of Soochow University, Suzhou, China.Show MeSH
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Mentions: Finally, to test the effects of miR-16 on glioma growth in vivo, we used a mouse model of human glioma. As shown in our experiments, all tumors from U87 cells transfected with plasmid-connected pre-miR-16 were efficiently suppressed compared with the negative control (Fig.5c,d). The intracranial tumors were removed and sectioned. Hematoxylin–eosin staining showed that miR-16 reduced glioma growth and invasion in the encephalic nude mouse model (Fig.5a). Immunofluorescence showed that miR-16 reduced glioma invasion and MMP9 expression in the nude encephalic mouse model (Fig.5b). Sections were stained for Ki-67, MMP9, and NF-κB1. Immunohistochemistry showed that tumors with U87 cells transfected with miR-16 reduced Ki-67 (Fig.6a), MMP9 (Fig.6b), and NF-κB1 stainings (Fig.6c), compared with negative control oligonucleotide. Thus, the data indicated that miR-16 upregulation led to the inhibition of glioma cell proliferation and invasion in tumor xenografts, and that the upregulation of miR-16 reduced glioma growth in the mouse model in vivo.
Affiliation: Department of Neurosurgery and Brain and Nerve Research Laboratory, The First Affiliated Hospital of Soochow University, Suzhou, China.